著者

水野 守道

出版者

日本結合組織学会

雑誌

Connective tissue (ISSN:0916572X)

巻号頁・発行日

vol.32, no.1, pp.69-75, 2000-03-25

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The regeneration of teeth and bone becomes to be a new therapy in the near future. Our group have been proposed that the extracellular matrix is an powerful stimulator for the regeneration of tissues. Recently we found that bone marrow cells which were harvested from rat femur, differentiated into osteoblasts when cells were cultured with type I collagen. Type II, III, and V collagen did not show the inducing activity. Therefore the inducing activity of type I collagen is specific. The induction by collagen was mediated by α2β1 integrin receptor of cells. The osteoblastic differentiation was inhibited by the interruption of the binding of collagen with α2β1 integrin. Type I collagen contributes to the osteoblastic differentiation at the early stage. At the late stage, bone sialoprotein(BSP)had a crucial role for the differentiation. When the binding of BSP with receptor was suppressed by the presence of the specific antibody, the expression of osteoblastic phenotypes of bone marrow cells were suppressed. Type I collagen enhanced the formation of mineralized tissues by dental pulp cells in the medium containing β-glycerophosphate. These findings imply that extracellular matrix facilitates the formation of mineralized tissues.

著者

中田 研 新城 宏隆 三山 崇英 前 達雄 濱田 雅之 史野 根生 堀部 秀二 越智 隆弘 吉川 秀樹

出版者

日本結合組織学会

雑誌

Connective tissue (ISSN:0916572X)

巻号頁・発行日

vol.32, no.3, pp.313-321, 2000-09-25

被引用文献数

2

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Meniscus is one of the cartilaginous tissues in the knee joints which plays important roles in joint motion, load transmission or lubrication. It is well known that intrinsic healing of injuredmeniscus is limited even if repaired surgically, and that degenerative joint disease develops following loss of its function. We investigated human and rat meniscus cells in the primary culture to characterize their potential of proliferation and gene expression of matrix proteins (type I, II, III, IX collagens and aggrecan), growth factors (TGF-β1, FGF-2, PDGF-A, IGF-II), and their receptors (TGF-βRI, FGFR1, PDGF-R, IFG-I-R). Furthermore, we studied the effect of these growth factors on cell proliferation. Finally, we investigated the possibility of meniscus transplantation with autogenous meniscus cells. Rat meniscus transplantation model was developed and collagen scaffold was used for meniscus cell seeding. These experiments showed future feasible strategy of meniscus cell therapy for meniscus injuries.