著者

白銀 隼人 宍戸 雅宏

出版者

千葉大学大学院園芸学研究科

雑誌

食と緑の科学 (ISSN:18808824)

巻号頁・発行日

no.67, pp.69-73, 2013-03

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White root rot of Japanse pear caused by Rosellinia necatrix was investigated in two orchards of the Center forEnvironment, Health and Field Sciences, Chiba University. The bait twig method coupled with PCR primers specificto the fungus indicated at least 28.9% of Japanese pear trees were infected with the pathogen. Because the detectionefficiency of the method is often less than 80 % , the disease was likey more prevalent than the detectedsamples in the orchards. Furthermore, Japanese pear trees that R. necatrix was detected had significantly shorternew shoots than trees that the pathogen was undetected, showing negative impact of the disease infection on thegrowth of host plants. Genetic polymorhisms of R. necatrix isolated from the orchard and other areas of Japan, inaddition to kin speceis: R. aquila and R. compacta, based on six UP-PCR primers exhibited no identical polymorphicpatterns among these Rosellinia isolates. This result suggests that R. necatrix of the orchard is genetically differentfrom isolates living in other areas of Japan.