著者

辻 彰子 石河 淳 井上 裕匡 Kudo Keiko Ikeda Noriaki

出版者

福岡医学会

雑誌

福岡医学雑誌 (ISSN:0016254X)

巻号頁・発行日

vol.96, no.3, pp.76-80, 2005-03-25

被引用文献数

1

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We performed a paternity test without the alleged father, who was deceased, and we found a mismatch in one of the alleles of the autosomal short tandem repeat (STR) locus D3S1358 in the illegitimate daughter. DNA genotypes of the dead alleged father were estimated from the DNA genotypes of his wife and their four children. The ABO genotype, the DXS10011 locus and the calculations using the 12 STR loci and the D1S80 locus, but not the STR locus D3S1358 suggested that there was a high probability that the dead alleged father was in fact the actual biological father. The genotype of locus D3S1358 of the dead alleged father, the illegitimate daughter's mother and the illegitimate daughter was 15/17, 15/17 and 15/18, respectively. PCR and sequencing after TA cloning of allele 17 of one of the children who had only a homozygote of allele 17, of allele 17 of the illegitimate daughter's mother and of allele 18 of the illegitimate daughter revealed that allele 18 which was considered to be a mutated allele had one more repeat of (GATA) within the first repeat region.擬父が既に死亡している親子鑑定を実施した.妻と4人の嫡出子のDNA型から推定した擬父の型と妻でない女性を母とした非嫡出子の12種類のSTR型においてD3S1358座位のみで不一致を認めた.ABO式血液型の遺伝子型とX-STR型のDXS10011座位およびD3S1358座位を除く他の座位におけるDNA型による父権肯定確率は擬父がこの非嫡出子の生物学的父親であることを示していた.D3S1358座位における遺伝子型は擬i父,非嫡出子の母共に15/17であったが,非嫡出子は15/18であった.i擬父の対立遺伝子17が遺伝していると考えられる娘と非嫡出子およびその母のD3S1358座位のクローニングおよびシークエンスにより,非嫡出子の対立遺伝子18は(GATA)の繰り返しが一回多い突然変異であることが判明した.