著者

原田 大輔 草島 佳紀 菅野 憲 遠田 浩司

出版者

The Japan Society for Analytical Chemistry

雑誌

分析化学 (ISSN:05251931)

巻号頁・発行日

vol.70, no.3, pp.207-213, 2021-03-05 (Released:2021-04-19)

参考文献数

14

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We developed lanthanoid-doped upconversion nanoparticles (UCNPs) and attempted to apply them as wavelength conversion devices to near-infrared luminescent sugar sensors. By doping yttrium fluoride with ytterbium and thulium, UCNPs (NaYF4: Yb/Tm) showing upconversion (UC) emission in the near-infrared region (800 nm) were synthesized. A receptor-sensitive near-infrared absorbing dye that increases the absorbance in the near-infrared region (700-800 nm) by complex formation with phenylboronic acid (PBA) as a sugar receptor was immobilized on core-shell type lanthanoid UCNPs covered with a silica layer. When fructose was added to a dye-immobilized core-shell type UCNPs dispersion solution containing a certain amount of PBA (1.0 × 10−3 mol L−1), the UC emission intensity at 800 nm increased with increasing the concentration of fructose (0 mol L−1 to 3.0 × 10−1 mol L−1). This result indicates that the internal filter effect between the dye and UCNPs is eliminated by a change in the absorption spectrum of the immobilized receptor-sensitive dye due to a competitive complex formation reaction accompanying the increase in the fructose concentration. And as a result, the UC emission intensity increased. It was found that when the fructose concentration was 3.0 × 10−1 mol L−1, the UC emission intensity increased by 14.6% compared to the case without fructose.