著者

菊池 太朗 中村 孝文 池田 天史 藤本 徹 坂本 吉弘 成尾 政一郎 高木 克公

出版者

West-Japanese Society of Orthopedics & Traumatology

雑誌

整形外科と災害外科 (ISSN:00371033)

巻号頁・発行日

vol.49, no.2, pp.515-519, 2000-03-25 (Released:2010-02-25)

参考文献数

5

被引用文献数

1

---

Many authors have reported that disc herniation can be resorbed by monocytic phagocytosis. However, how the recruitment of monocytes is triggered is still unknown.We investigated monocyte chemoattractant protein-1 (MCP-1) by using the experimental rat model in vivo and rabbit model in vitro to ascertain the precise mechanism of macrophage recruitment in the early phase of disc resorption.The autologous intervertebral discs from tails of Wister rat were subcutaneously implanted into the abdomen. These discs were used for immunohistochemical study. Additionally, we employed tissue culture models of rabbit's discs, and the quantification of MCP-1 in these media was performed.MCP-1 positive disc chondrocytes were observed in the nucleus pulposus and the inner annulus fibrosis on the third day. Monocyte derived macrophages were considerably observed around the intervertebral disc by the seventh day. The MCP-1 induced by rrlL-1β or rrTNFα indicated its peak volume at 12 hours and at 18 hours after incubation.Our study suggests that intervertebral disc chondrocytes have chemotactic properties and play an active role in the recruitment of monocytes involved in disc resorption. Moreover, inflammatory cytokines induce and regulate the production of MCP-1 in the intervertebral disc cells.