著者

Yosuke AMAGAI Akane TANAKA Akira MATSUDA Kumiko OIDA Kyungsook JUNG Sho NISHIKAWA Hyosun JANG Saori ISHIZAKA Hiroshi MATSUDA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.13-0025, (Released:2013-02-22)

被引用文献数

1 4

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Myeloid cell leukemia sequence 1 (MCL1) is a potent antiapoptotic protein that plays a critical role in cell survival and drug resistance in various cancers. However, to the best of our knowledge, the role of MCL1 in mast cell tumors (MCTs) has not been investigated in dogs. Here, we detected increased MCL1 expression in MCT cell lines, regardless of the presence of a c-kit mutation. MCL1 expression increased when the cells were exposed to specific inhibitors of mitogen-activated protein kinase or Janus kinase-signaling pathways, thus protecting the cells from apoptosis, but not when KIT or phosphatidylinositol-3 kinase signaling cascades were inhibited. These results indicate that MCL1 expression may contribute to MCT survival and confer drug resistance.

著者

Izumi ANDO Kaoru KARASAWA Hiroshi MATSUDA Akane TANAKA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.16-0258, (Released:2016-07-22)

被引用文献数

8

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Serum nerve growth factor (NGF) levels are increased by the external stress in mice, humans and horses; however, similar variations have been unclear in dogs. Since dogs are usually subjected to conditions of work, exercise and activity as important partners of humans, we measured serum NGF levels post-exercise and compared them with serum cortisol levels, as a biomarker of physical stress. Serum cortisol levels were immediately elevated post-exercise and returned to basal levels within 1 hr. On the other hand, serum NGF levels were significantly increased 1 hr post-exercise and gradually returned to basal levels. Further research is necessary; nevertheless, we have demonstrated for the first time that serum NGF levels respond to exercise stress in dogs.

著者

Yosuke AMAGAI Akane TANAKA Akira MATSUDA Kumiko OIDA Kyungsook JUNG Hiroshi MATSUDA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.12-0540, (Released:2013-01-17)

被引用文献数

4 7 2

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Mast cell tumors (MCTs) are the most common tumors in dogs, accounting for 16–21% of cutaneous tumors. Although several small molecule inhibitors, including imatinib mesylate, have been used for the treatment of MCTs, the response rate remains less than 50%. In this study, the effects of different selective signal inhibitors on MCT cell growth were evaluated using 4 different cell lines derived from dogs. We found that the phosphoinositide 3-kinase (PI3K) signaling pathway is crucial for the proliferation of MCT cells in the presence or absence of c-kit gene mutations. Here, we propose a novel therapeutic strategy to target the PI3K pathway for the treatment of canine MCTs.

著者

Yosuke AMAGAI Akane TANAKA Akira MATSUDA Kumiko OIDA Kyungsook JUNG Sho NISHIKAWA Hyosun JANG Saori ISHIZAKA Hiroshi MATSUDA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.75, no.7, pp.971-974, 2013 (Released:2013-07-31)

参考文献数

25

被引用文献数

1 4

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Myeloid cell leukemia sequence 1 (MCL1) is a potent antiapoptotic protein that plays a critical role in cell survival and drug resistance in various cancers. However, to the best of our knowledge, the role of MCL1 in mast cell tumors (MCTs) has not been investigated in dogs. Here, we detected increased MCL1 expression in MCT cell lines, regardless of the presence of a c-kit mutation. MCL1 expression increased when the cells were exposed to specific inhibitors of mitogen-activated protein kinase or Janus kinase-signaling pathways, thus protecting the cells from apoptosis, but not when KIT or phosphatidylinositol-3 kinase signaling cascades were inhibited. These results indicate that MCL1 expression may contribute to MCT survival and confer drug resistance.