著者

Tetsuya FURUYA Akiko WACHI Yukiko SASSA Tsutomu OMATSU Makoto NAGAI Ryuji FUKUSHIMA Makoto SHIBUTANI Tomohiro YAMAGUCHI Yosuke UEMATSU Kinji SHIROTA Tetsuya MIZUTANI

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.77, no.12, pp.1701-1703, 2015 (Released:2016-01-01)

参考文献数

5

被引用文献数

2 16

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Feline morbillivirus (FmoPV) is a new virus species and its detection is important, since correlation has been reported between FmoPV virus infection and tubulointerstitial nephritis in cats. Here, we report a real-time reverse transcription (RT)-PCR system that can detect the FmoPV L-gene sequence with more than 10-time higher sensitivity than a conventional PCR system, resulting in detection of less than 10 copies of the template DNA. The total FmoPV positive rate of urine samples from veterinary clinics and hospitals in Japan was 15.1% (25/166) using this system. This study demonstrates usefulness of the real-time RT-PCR system for detection of FmoPV for cat urine samples.

著者

Tetsuya FURUYA Akiko WACHI Yukiko SASSA Tsutomu OMATSU Makoto NAGAI Ryuji FUKUSHIMA Makoto SHIBUTANI Tomohiro YAMAGUCHI Yosuke UEMATSU Kinji SHIROTA Tetsuya MIZUTANI

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.15-0112, (Released:2015-07-26)

被引用文献数

2 16

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Feline morbillivirus (FmoPV) is a new virus species and its detection is important, since correlation has been reported between FmoPV virus infection and tubulointerstitial nephritis in cats. Here, we report a real-time reverse transcription (RT)-PCR system that can detect the FmoPV L-gene sequence with more than 10-time higher sensitivity than a conventional PCR system, resulting in detection of less than 10 copies of the template DNA. The total FmoPV positive rate of urine samples from veterinary clinics and hospitals in Japan was 15.1% (25/166) using this system. This study demonstrates usefulness of the real-time RT-PCR system for detection of FmoPV for cat urine samples.