文献一覧: Akiyoshi TANI (著者)

2 0 0 0 OA Changes in gene expression profiles and cytokine secretions in peripheral monocytes by treatment with small extracellular vesicles derived from a canine lymphoma cell line

著者: Akiyoshi TANI Hirotaka TOMIYASU Hajime ASADA Chen-Si LIN Yuko GOTO-KOSHINO Koichi OHNO Hajime TSUJIMOTO
出版者: JAPANESE SOCIETY OF VETERINARY SCIENCE
雑誌: Journal of Veterinary Medical Science (ISSN:09167250)
巻号頁・発行日: pp.21-0506, (Released:2022-04-06)
被引用文献数: 3

Interactions between tumor and immune cells within the tumor microenvironment play an important role in tumor progression, and small extracellular vesicles (EVs) derived from these tumor cells have been shown to exert immunomodulatory effects on various immune cells, including macrophages and lymphocytes. Although the immunomodulatory effects of small EVs derived from human cancer cells have been intensively investigated, few studies have investigated the effects of lymphoma-derived small EVs on macrophages in both human and veterinary medicine. Here, we evaluated the effects of canine lymphoma-derived small EVs on canine primary monocytes, which are the major source of macrophages in neoplastic tissues. Comprehensive gene expression analysis of these treated monocytes revealed their distinct activation via the Toll-like receptor (TLR) and NF-κβ signaling pathways. In addition, treatment with lymphoma small EVs increased the secretion of MCP-1, which induces the infiltration and migration of monocytes and lymphocytes in neoplastic and cancer tissues. The results of this study indicate that canine lymphoma small EVs activate monocytes, possibly through the activation of TLR and NF-κβ signaling pathways, and induce monocytes to secrete of MCP-1, which might contribute to immune cell infiltration within the tumor microenvironment.

2022-04-07 13:11:19
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1 0 0 0 OA Gene expression profiles associated with early relapse during first remission induction in canine multicentric high-grade B-cell lymphoma

著者: Hiroto TOYODA Akiyoshi TANI Yuko GOTO-KOSHINO Tomoki MOTEGI Mika SAKAMOTO Takako MOCHIZUKI Kei HARADA Tetsuya KOBAYASHI Asuka SETOGUCHI Yohei SHIZUTA Takuya MIZUNO Mitsuhiro IRIE Jun NAKAMICHI Hajime TSUJIMOTO Aki OHMI Ray FUKUOKA Yasukazu NAKAMURA Hirotaka TOMIYASU
出版者: JAPANESE SOCIETY OF VETERINARY SCIENCE
雑誌: Journal of Veterinary Medical Science (ISSN:09167250)
巻号頁・発行日: vol.86, no.1, pp.18-27, 2024 (Released:2024-01-10)
参考文献数: 49
被引用文献数: 1

Although chemotherapy using CHOP-based protocol induces remission in most cases of canine multicentric high-grade B-cell lymphoma (mhBCL), some cases develop early relapse during the first induction protocol. In this study, we examined the gene expression profiles of canine mhBCL before chemotherapy and investigated their associations with early relapse during the first whole CHOP-based protocol. Twenty-five cases of mhBCL treated with CHOP-based protocol as first induction chemotherapy were included in this study. Sixteen cases completed the first whole CHOP-based protocol without relapse (S-group), and nine developed relapse during the chemotherapy (R-group). RNA-seq was performed on samples from neoplastic lymph nodes. Differentially expressed genes (DEGs) were extracted by the comparison of gene expression profiles between S- and R-groups, and the differences in the expression levels of these genes were validated by RT-qPCR. Extracted 179 DEGs included the genes related to chemokine CC motif ligand, T-cell receptor signaling pathway, and PD-L1 expression and PD-1 checkpoint pathway. We focused on chemokine CC motif ligand, and CCL4 was confirmed to be significantly downregulated in the R-group (P=0.039). We also focused on the genes related to T-cell signaling pathway, and CD3E (P=0.039), ITK (P=0.023), and LAT (P=0.023) genes were confirmed to be significantly upregulated in the R-group. The current results suggest that both changes in tumor cells and the interactions between tumor cells and immune cells are associated with the efficacy of the chemotherapy for first remission induction.

2024-01-11 03:56:17
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1 0 0 0 OA Gene expression profiles associated with early relapse during first remission induction in canine multicentric high-grade B-cell lymphoma

著者: Hiroto TOYODA Akiyoshi TANI Yuko GOTO-KOSHINO Tomoki MOTEGI Mika SAKAMOTO Takako MOCHIZUKI Kei HARADA Tetsuya KOBAYASHI Asuka SETOGUCHI Yohei SHIZUTA Takuya MIZUNO Mitsuhiro IRIE Jun NAKAMICHI Hajime TSUJIMOTO Aki OHMI Ray FUKUOKA Yasukazu NAKAMURA Hirotaka TOMIYASU
出版者: JAPANESE SOCIETY OF VETERINARY SCIENCE
雑誌: Journal of Veterinary Medical Science (ISSN:09167250)
巻号頁・発行日: pp.23-0269, (Released:2023-11-13)
被引用文献数: 1

2023-11-13 20:05:19
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1 0 0 0 OA Clinical and clinicopathological features and outcomes of Miniature Dachshunds with bone marrow disorders

著者: Akiyoshi TANI Hirotaka TOMIYASU Aki OHMI Koichi OHNO Hajime TSUJIMOTO
出版者: JAPANESE SOCIETY OF VETERINARY SCIENCE
雑誌: Journal of Veterinary Medical Science (ISSN:09167250)
巻号頁・発行日: pp.19-0439, (Released:2020-04-17)
被引用文献数: 6

Non-neoplastic bone marrow disorders such as non-regenerative immune-mediated anemia, pure red cell aplasia, and myelodysplastic syndrome are major causes of non-regenerative anemia in dogs. However, there has been no study on the clinical and clinicopathological features of canine non-neoplastic bone marrow disorders in Japan. Hence, we first investigated the breed disposition of non-neoplastic bone marrow disorders that induce anemia as a retrospective study and found that Miniature Dachshund (MD) was a predisposed breed. Based on this finding, we investigated the clinical and clinicopathological features of non-neoplastic bone marrow disorders in MDs as a preliminary retrospective study, and we compared them between immunosuppressive treatment-responsive and -resistant MDs. We found that treatment-resistant MDs showed thrombocytosis and increased frequencies of dysplastic features in the peripheral blood. These results indicate that bone marrow disorders in treatment-resistant MDs might manifest distinct features compared with those in treatment-sensitive MDs, and sensitivity to immunosuppressive treatments could be predicted based on thrombocytosis and dysplastic features in the peripheral blood. Further studies that examine aberrations in the genome are needed to elucidate the pathophysiology of bone marrow disorders in MDs.

2020-04-17 21:03:17
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1 0 0 0 OA Comparison of the efficacy of cyclosporine and leflunomide in treating inflammatory colorectal polyps in miniature dachshunds

著者: Akiyoshi TANI Taisuke SENO Nozomu YOKOYAMA Taisuke NAKAGAWA Hirotaka TOMIYASU Yuko GOTO-KOSHINO Hajime TSUJIMOTO Koichi OHNO
出版者: JAPANESE SOCIETY OF VETERINARY SCIENCE
雑誌: Journal of Veterinary Medical Science (ISSN:09167250)
巻号頁・発行日: pp.19-0560, (Released:2020-02-14)
被引用文献数: 1

Inflammatory colorectal polyps (ICRPs) are frequently observed in miniature dachshunds in Japan and treated by prednisolone and immunosuppressive drugs such as cyclosporine and leflunomide. The purpose of this retrospective study was to compare the treatment efficacy, such as response rate, response interval, recurrence rate, and adverse events between cyclosporine and leflunomide. While the response rates were significantly higher in dogs treated with leflunomide, no significant differences were observed in the response interval or recurrence rate. Two of the 11 dogs treated with leflunomide showed hematological or gastrointestinal adverse events, while no dog treated with cyclosporine showed any adverse events. A case-controlled prospective study to compare the treatment efficacy of leflunomide with that of cyclosporine should be conducted.

2020-02-15 06:50:39
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