- 著者
- YASUKO WATANABE SACHIKO SHIBATA BONRO KOBAYASHI
- 出版者
- The Japan Endocrine Society
- 雑誌
- Endocrinologia Japonica (ISSN:00137219)
- 巻号頁・発行日
- vol.16, no.1, pp.133-147, 1969 (Released:2011-01-25)
- 参考文献数
- 33
dependence of the magnitude of serotonin creatinine sulfate-induced swelling upon the inducer concentration was evident down to 5×10-6M. Possibility of heavy metal contamination was excluded by the use of ash obtained after combustion of the serotonin preparation employed. Serotonin hydrochloride prepared by a novel Sephadex G-10 column method was shown to induce mitochondrial swelling. Sulfate or creatinine sulfate enhanced the swelling activity of serotonin. The time-course of serotonin-induced swelling simulated that of the swelling induced by NAD-linked substrate of mitochondrial oxidation. On a molar basis, serotonin was found to be more active than the NAD-linked substrates or some of the related indole derivatives. NAD-linked substrates interacted synergistically, and Ca2+ interacted additively with serotonin in the induction of swelling. Succinate markedly counteracted the serotonin effect. Serotonin-induced swelling was reversed by ATP, without an requirement of extraneous Mg2+. It was prevented by serum albumin, rotenone, amytal, antimycin A, dinitrophenol, and cyanide, but could not be prevented by a monoamine oxidase inhibitor β-isopropylphenylhydrazine. It differed from ascorbate-induced lysis in both the time course and magnitude. Ascorbate-induced mitochondrial lysis was prevented by serotonin. Dependence of serotonin-induced swelling on the NAD-branch of the respiratory chain was proposed and possible mechanism of the action was discussed.
- 著者
- BONRO KOBAYASHI MICHIO UI YASUKO WARASHINA
- 出版者
- The Japan Endocrine Society
- 雑誌
- Endocrinologia Japonica (ISSN:00137219)
- 巻号頁・発行日
- vol.7, no.3, pp.225-238, 1960 (Released:2011-01-25)
- 参考文献数
- 42
- 被引用文献数
- 11 15
Intravenous injection of serotonin into intact rats caused 2 stages of glycemic response, i. e., initial hypoglycemia followed by marked hyperglycemia. Hyperglycemia was found to be due to the release of epinephrine from adrenal medulla since adrenodemedullated rats showed virtually no hyperglycemic response. Adrenodemedullation as well as anesthesia with sodium pentobarbital sensitized rats to hypoglycemic action of serotonin. Hypoglycemic response was roughly proportional to the dosage, and as low as 50μg per rat resulted in significant hypoglycemia. Subcutaneous and intraperitoneal serotonin showed comparable but less effect. Hypoglycemic effect of serotonin was additive to that of insulin and inhibited epinephrine induced hyperglycemia. Diaphragm of rats injected with serotonin prior to the sacrifice showed increased glucose uptake when incubated in vitro. Glucose as well as inulin tolerance test showed characteristic pattern of concentration curves. Chronically serotonin-treated rats showed increased insulin sensitivity and a little improved glucose tolerance than the control littermates. Possible role of serotonin in carbohydrate metabolism was discussed in conjunction with several reports by other investigators.