著者

YASUKO WATANABE SACHIKO SHIBATA BONRO KOBAYASHI

出版者

The Japan Endocrine Society

雑誌

Endocrinologia Japonica (ISSN:00137219)

巻号頁・発行日

vol.16, no.1, pp.133-147, 1969 (Released:2011-01-25)

参考文献数

33

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dependence of the magnitude of serotonin creatinine sulfate-induced swelling upon the inducer concentration was evident down to 5×10-6M. Possibility of heavy metal contamination was excluded by the use of ash obtained after combustion of the serotonin preparation employed. Serotonin hydrochloride prepared by a novel Sephadex G-10 column method was shown to induce mitochondrial swelling. Sulfate or creatinine sulfate enhanced the swelling activity of serotonin. The time-course of serotonin-induced swelling simulated that of the swelling induced by NAD-linked substrate of mitochondrial oxidation. On a molar basis, serotonin was found to be more active than the NAD-linked substrates or some of the related indole derivatives. NAD-linked substrates interacted synergistically, and Ca2+ interacted additively with serotonin in the induction of swelling. Succinate markedly counteracted the serotonin effect. Serotonin-induced swelling was reversed by ATP, without an requirement of extraneous Mg2+. It was prevented by serum albumin, rotenone, amytal, antimycin A, dinitrophenol, and cyanide, but could not be prevented by a monoamine oxidase inhibitor β-isopropylphenylhydrazine. It differed from ascorbate-induced lysis in both the time course and magnitude. Ascorbate-induced mitochondrial lysis was prevented by serotonin. Dependence of serotonin-induced swelling on the NAD-branch of the respiratory chain was proposed and possible mechanism of the action was discussed.

著者

FUMIO UMEDA KEN-ICHI KATO KAZUO MUTA HIROSHI IBAYASHI

出版者

The Japan Endocrine Society

雑誌

Endocrinologia Japonica (ISSN:00137219)

巻号頁・発行日

vol.29, no.3, pp.287-292, 1982 (Released:2011-01-25)

参考文献数

23

被引用文献数

19 22

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The role of vitamin E in the endocrine system, in particular the pituitary-gonadal axis, was studied in humans and male rats by examining the hormonal differences between vitamin E deficient and supplemented conditions.In vitamin E deficient rats, pituitary content and basal plasma level of FSH and LH were significantly lower than those of the control rats, but testicular content and basal plasma level of testosterone were not significantly changed.On the other hand, in vitamin E supplemented rats, FSH and LH content in pituitary tissue was significantly higher than that of the controls, but there was no significant rise in basal FSH and LH level in plasma. The testosterone level was significantly elevated in both testicular tissue and plasma. It was also demonstrated that basal plasma testosterone and F. T. I. were increased in normal male subjects following oral vitamin E administration and the responsiveness of plasma testosterone levels to HCG was significantly higher during vitamin E administration than before administration.These results suggest that vitamin E may play an important and potent role in hormone production in the pituitary-gonadal axis in humans and rats.

著者

IZAWA MASAO

出版者

The Japan Endocrine Society

雑誌

Endocrinologia Japonica (ISSN:00137219)

巻号頁・発行日

vol.37, no.2, pp.233-238, 1990

被引用文献数

2

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When the <I>in vitro</I> translation products of mRNAs from castrated animals (48h) were compared with those from androgen-treated animals (48 h) to survey the molecular mechanism of androgen-responsive gene expressions in the rat seminal vesicles, some peptide bands which were repressed in response to androgen were observed. From these findings, we constructed a partial cDNA library of poly (A+) RNAs which had been isolated from the seminal vesicles of castrated rats (48 h) and modestly enriched with respect to the concentration of androgen-repressed mRNAs by sucrose density gradient centrifugation, and screened by differential colony hybridization. One cDNA clone, pSvr-1, whose mRNA is markedly induced within 24h after castration of the animal in the seminal vesicles as well as in the ventral prostate, was isolated. pSvr-1 hybridized to a mRNA of 1, 700 nucleotides in length. Partial sequence analysis showed that this clone had highly homologous but not identical sequences to those reported for rat sulfated glycoprotein-2. This cDNA clone may provide a useful probe for the study of the negative regulation mechanism of gene expression by androgens.

著者

HISANORI MINAKAMI Kozo KIMURA KUNIHIKO IJIMA TARO TAMADA

出版者

The Japan Endocrine Society

雑誌

Endocrinologia Japonica (ISSN:00137219)

巻号頁・発行日

vol.32, no.5, pp.645-651, 1985 (Released:2011-01-25)

参考文献数

35

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Although estrogen is known to stimulate the secretion of prolactin, there are only slight differences between the prolactin levels in the follicular and luteal phases in normal women. To test the hypothesis that progesterone is involved in the regulation of prolactin release, 50mg of progesterone was administered intramuscularly at 0600h to twelve hypogonadal women and blood samples were obtained at 15min intervals between 1500 and 2000h to determine the prolactin levels. The day before progesterone treatment, control blood samples were obtained at 15 min intervals between 1500 and 2000h. The serum progesterone levels were 28.7±4.1ng/ml at 1500h, 24.2±3.5ng/ml at 1730h and 21.3±2.9ng/ml (mean±SD) at 2000h. In eight of twelve hypogonadal women, progesterone lowered circulating prolactin levels significantly. These results indicate that a high level of progesterone in the luteal phase may partly block estrogen-induced prolactin release physiologically.

著者

YUKICHI OKUDA KOICHI KAWAI YASUKO MURAYAMA KAMEJIRO YAMASHITA

出版者

The Japan Endocrine Society

雑誌

Endocrinologia Japonica (ISSN:00137219)

巻号頁・発行日

vol.34, no.3, pp.415-422, 1987 (Released:2011-01-25)

参考文献数

17

被引用文献数

14 16

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We have examined changes in plasma ketone bodies and their related metabolites after the ingestion of a mixed meal in normal and NIDDM (non-insulindependent diabetes mellitus) using a highly sensitive colorimetric method. In normal subjects, fasting plasma acetoacetic acid (AcAc) and 3-β-hydroxybutyric acid (3-OHB) concentrations were 40.2±2.9 and 21.3±4.0μM, respectively. The total carnitine level in fasting plasma was 48.2±3.2μM and acyl/free was 0.34±0.12. These values did not change significantly after the meal. In diabetic subjects. fasting AcAc and 3-OHB levels were 57.9±3.5 and 97.9±14.7μM, respectively and these values especially 3-OHB, decreased almost to the normal level by 4h after the meal. The total carnitine level in the tasting plasma of diabetics was 48.7±2.8μM and acyl/free was 0.58±0.09. Such characteristics were observed when their plasma glucose had been completely normal for more than 10days. In patients treated with sulfonylurea, the fasting ketone body level, especially 3-OHB, was significantly lower than that of diabetics treated with insulin or diet alone, whereas the fasting plasma glucose level and its postprandial increase were higher than those of the others. These results demonstrate that measurement of the plasma ketone body (especially 3-OHB) level is a sensitive tool for monitoring the metabolic status of NIDDM.

著者

RYOYU TAKEDA SHINPEI MORIMOTO KENZO UCHIDA TSUGUHIKO NAKAI MASAJI MIYAMOTO TETSUJI HASHIBA KOHEI YOSHIMITSU KYUNG SU KIM UMEO MIWA

出版者

The Japan Endocrine Society

雑誌

Endocrinologia Japonica (ISSN:00137219)

巻号頁・発行日

vol.26, no.5, pp.541-547, 1979 (Released:2011-01-25)

参考文献数

15

被引用文献数

15 24

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We describe the natural recovery from the aggravated hypertension, hypokalemia and suppression of the renin-aldosterone axis after the glycyrrhizin discontinuation in two mild hypertensive women aged 71 and 68 years, who had been administered 273 to 546mg glycyrrhizin daily for 1.5 and 6 months, respectively, for the treatment of liver disease. About one month after the glycyrrhizin discontinuation, acceleration of hypertension, hypokalemia and suppression of the renin-aldosterone system still continued in both patients. At this stage, sodium restriction resulted in the normalization of blood pressure with weight loss and the subsequent sodium repletion produced a rapid increase in blood pressure to hypertensive levels observed before sodium restriction, with weight gain. Plasma renin activity and plasma aldosterone were low and did not respond to sodium restriction. Inappropriately excessive amounts of potassium were also excreted in the presence of hypokalemia. About one and a half months later, the improvements of aggravated hypertension, hypokalemia and suppressed renin-aldosterone system gradually occurred in both patients. Sodium restriction performed about three months later in case 2 no longer produced the changes in blood pressure and body weight. Plasma renin activity and plasma aldosterone responded subnormally to sodium restriction.These results demonstrate that both patients had a prolongation of the syndrome resembling primary aldosteronism except the low plasma aldosterone level about one month after the glycyrrhizin discontinuation. The possible mechanisms by which this prolongation was caused are discussed.

著者

BONRO KOBAYASHI MICHIO UI YASUKO WARASHINA

出版者

The Japan Endocrine Society

雑誌

Endocrinologia Japonica (ISSN:00137219)

巻号頁・発行日

vol.7, no.3, pp.225-238, 1960 (Released:2011-01-25)

参考文献数

42

被引用文献数

11 15

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Intravenous injection of serotonin into intact rats caused 2 stages of glycemic response, i. e., initial hypoglycemia followed by marked hyperglycemia. Hyperglycemia was found to be due to the release of epinephrine from adrenal medulla since adrenodemedullated rats showed virtually no hyperglycemic response. Adrenodemedullation as well as anesthesia with sodium pentobarbital sensitized rats to hypoglycemic action of serotonin. Hypoglycemic response was roughly proportional to the dosage, and as low as 50μg per rat resulted in significant hypoglycemia. Subcutaneous and intraperitoneal serotonin showed comparable but less effect. Hypoglycemic effect of serotonin was additive to that of insulin and inhibited epinephrine induced hyperglycemia. Diaphragm of rats injected with serotonin prior to the sacrifice showed increased glucose uptake when incubated in vitro. Glucose as well as inulin tolerance test showed characteristic pattern of concentration curves. Chronically serotonin-treated rats showed increased insulin sensitivity and a little improved glucose tolerance than the control littermates. Possible role of serotonin in carbohydrate metabolism was discussed in conjunction with several reports by other investigators.

著者

KIICHIRO HIGASHI MITSUO MORITA JUNICHI TAJIRI TATSUO SATO KOSHIRO OKAZAKI SUMIO ARAI

出版者

The Japan Endocrine Society

雑誌

Endocrinologia Japonica (ISSN:00137219)

巻号頁・発行日

vol.32, no.3, pp.421-426, 1985 (Released:2011-01-25)

参考文献数

13

被引用文献数

1

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In order to obtain a good separation line between patients with primary hyperparathyroidism (1°HPT) and those with non-parathyroidal hypercalcemia (NPHC), serum chloride (Cl) and phosphate (P) concentrations were analyzed. Ninety-nine per cent of the patients with 1°HPT had a Cl/P ratio greater than or equal to 33, but 29% of patients with NPHC were also included in this range. When the (Cl-90)/P ratio was used to separate into two groups, 98% of the patients with 1°HPT had a ratio greater than or equal to 5.0, and 94% of the patients with NPHC had a ratio less than 5.0. From these results, while high sensitivity was achieved both with the Cl/P and (Cl-90)/P ratios, the (Cl-90)/P ratio provided higher specificity. Therefore we concluded that the (Cl-90)/P ratio was excellent in distinguishing 1°HPT from other types of hypercalcemia.

著者

MICHINORI KABUTO IKUO NAMURA YOUICHI SAITOH

出版者

The Japan Endocrine Society

雑誌

Endocrinologia Japonica (ISSN:00137219)

巻号頁・発行日

vol.33, no.3, pp.405-414, 1986 (Released:2011-01-25)

参考文献数

14

被引用文献数

35 45

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Plasma melatonin levels were determined every 20 and 30min for 24 hours on the last day of repeated oral administrations (1 or 2mg a day for 8 or 9 days) of a benzodiazepine derivative (450191-s), which is known to be metabolized to active benzodiazepines after administration. In one of the two subjects, the nocturnal enhancement of plasma melatonin which was obvious on a control day with placebo was diminished almost completely. In the other subject, observed were not only the diminishment of its nocturnal enhancement but also its increase during the daytime almost to the nocturnal levels on a control day, which may indicate a rebound increase in melatonin synthesis or a shift in its day-night rhythmicity.Such suppressing effects of benzodiazepines on the nocturnal plasma melatonin levels were also examined in the case of a single administration of 2mg of 450191-s or flunitrazepam in the second series of experiments. Even a single flunitrazepam seemed to have lowered nocturnal plasma melatonin levels, which then recovered to the usual levels following the administration of 5mg of a benzodiazepine antagonist, Ro 15-1788, given 6 hours after the flunitrazepam. However, single 450191-s did not show any remarkable effects.Thus, it has been suggested that benzodiazepines could suppress the nocturnal levels of plasma melatonin or shift its day-night rhythmicity at least when administered repeatedly. The possible action site of benzodiazepines may be the central nervous system, since melatonin synthesis has been thought to be under strongly regulated by the central nervous pathway from the retina to the pineal body. Therefore, these effects of benzodiazepines may provide a method for investigating the physiological role of melatonin and its day-night rhythmicity as well as to further clarify the system regulating melatonin synthesis in humans.

著者

IZAWA MASAO

出版者

The Japan Endocrine Society

雑誌

Endocrinologia Japonica (ISSN:00137219)

巻号頁・発行日

vol.38, no.1, pp.61-66, 1991

被引用文献数

4

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To further survey the molecular mechanisms underlying the involution of steroid hormone-dependent rat tissues, we undertook experiments to test whether or not any significant correlation between the tissue involution and expressions of rat sulfated glycoprotein 2 (SGP-2) and pSvr-1 genes, which had been initially cloned from the Sertoli cells and the seminal vesicles, respectively, and then identified as androgen repressed messages both in the ventral prostate and in the seminal vesicles, could be observed in steroid hormone-dependent rat tissues. Expressions of these genes were stimulated within 48 h after castration of animals both in the ventral prostate and in the seminal vesicles as reported previously, but not significantly altered by ovariectomy in the uterus. Expressions of these genes in the thymus were significantly repressed by the administration of dexamethasone and/or cycloheximide. Although the roles of expressions of SGP-2 and pSvr-1 genes in steroid hormonedependent tissues remain unclear, their presence might become useful molecular markers of tissue involution not only in androgen-dependent rat tissues but also in glucocorticoid-dependent ones, and also provide excellent model systems for the study of negative regulation mechanism of gene expression by steroid hormones.

著者

TAKESHI KUZUYA YASUNORI KANAZAWA MITSUAKI HAYASHI MASATOSHI KIKUCHI TAKEHIKO IDE

出版者

The Japan Endocrine Society

雑誌

Endocrinologia Japonica (ISSN:00137219)

巻号頁・発行日

vol.18, no.4, pp.309-320, 1971 (Released:2011-01-25)

参考文献数

16

被引用文献数

4 16

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Glucose and xylitol were administered intravenously to man and several mammals in the same doses, and the plasma insulin responses were compared. Marked species differences were found. In man, 12g or 30g of glucose produced definitely greater rises of plasma insulin than xylitol. In cows and goats, both 0.2g/kg glucose and xylitol increased plasma insulin markedly and to a similar extent. Plasma insulin responses of horses to 0.2g/kg glucose and xylitol were rather sluggish and poor. In rabbits, the peak insulin levels following 0.2 g/kg glucose and xylitol were approximately similar or slightly higher after glucose, but with the dose of 1, 0g/kg, the peak plasma insulin value after xylito was a little higher than after glucose. In rats, glucose produced higher insulin peaks than xylitol both at the doses of 0.2g/kg and 1.0g/kg. These results are in contrast to the results obtained in dogs, in which xylitol has a definitely stronger insulin-releasing effect than glucose. The cause for this species variation is not explained at present.