- 著者
- Masayo SAKATA Takanori SUEDA Hirotaka IHARA Chuichi HIRAYAMA
- 出版者
- The Pharmaceutical Society of Japan
- 雑誌
- Chemical and Pharmaceutical Bulletin (ISSN:00092363)
- 巻号頁・発行日
- vol.44, no.2, pp.328-332, 1996-02-15 (Released:2008-03-31)
- 参考文献数
- 16
- 被引用文献数
- 2 3
Novel copolymeric adsorbents for the selective removal of endotoxin from an acidic protein solution were prepared. The adsorbents comprise spherical copolymers derived from N, N-dimethylaminopropylacrylamide (DMAPAA) and divinylbenzene (DVB). When the molar ratio of DMAPAA to DVB was 80/20 (amino-group content : 5.1 meq/g) and the pore size (molecular mass exclusion of polysaccharide, Mlim) was 4000 to 10000, DMAPAA/DVB showed high endotoxin-adsorbing activity at pH 5.0 to 9.0 and ionic strengths of μ=0.05 to 0.4. The capacity of the adsorbent (Mlim : 4000) was 390 μg of endotoxin (lipopolysaccharide purified from E. coli O111 : B4) per ml of the adsorbent using the batchwise method. The apparent dissociation constant between endotoxin and the adsorbent was 2.2×10-12 M. On the other hand, the adsorption of bovine serum albumin, an acidic protein, by the adsorbent increased with an increase in Mlim from 4000 to 10000, but decreased with an increase in ionic strength (μ) from 0.05 to 0.2 As a result, DMAPAA/DVB (80/20)(Mlim : 4000)selectively removed endotoxin from various acidic protein solutions at pH 7.0 and μ=0.05. The residual concentration of endotoxin in the protein solution always decreased to a concentration lower than 0.1 ng/ml, and recovery of the protein was more than 97%.
- 著者
- Chuichi HIRAYAMA Masayo SAKATA Hirotaka IHARA Kunio OHKUMA Makoto IWATSUKI
- 出版者
- The Japan Society for Analytical Chemistry
- 雑誌
- Analytical Sciences (ISSN:09106340)
- 巻号頁・発行日
- vol.8, no.6, pp.805-810, 1992-12-10 (Released:2006-06-30)
- 参考文献数
- 16
- 被引用文献数
- 13 13
A novel adsorbent for the selective removal of endotoxin from various protein solutions has been prepared. The adsorbent comprises aminated poly(γ-methyl L-glutamate) (PMLG) spheres. The larger is the number of incorporated amino groups, the larger is the endotoxin-adsorption capacity of the adsorbent, the smaller is the pore size (exclusion of molecular weight; Mlim) of the adsorbents, the smaller is the number of various proteins adsorbed. When Mlim was smaller than 300 (as the molecular weight of polysaccharide) and the amino-group content was 3.2meq/g, the adsorbent selectively removed endotoxin from various endotoxin-containing samples, such as protein solutions, without affecting the effective substances, even at such a high ionic strength (μ) of 0.2-0.4. Consequently, aminated PMLG spheres with a certain pore size are considered to be a useful adsorbent for the removal of endotoxin from drugs and fluids for injection.