著者

Chuichi HIRAYAMA Masayo SAKATA Hirotaka IHARA Kunio OHKUMA Makoto IWATSUKI

出版者

The Japan Society for Analytical Chemistry

雑誌

Analytical Sciences (ISSN:09106340)

巻号頁・発行日

vol.8, no.6, pp.805-810, 1992-12-10 (Released:2006-06-30)

参考文献数

16

被引用文献数

13 13

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A novel adsorbent for the selective removal of endotoxin from various protein solutions has been prepared. The adsorbent comprises aminated poly(γ-methyl L-glutamate) (PMLG) spheres. The larger is the number of incorporated amino groups, the larger is the endotoxin-adsorption capacity of the adsorbent, the smaller is the pore size (exclusion of molecular weight; Mlim) of the adsorbents, the smaller is the number of various proteins adsorbed. When Mlim was smaller than 300 (as the molecular weight of polysaccharide) and the amino-group content was 3.2meq/g, the adsorbent selectively removed endotoxin from various endotoxin-containing samples, such as protein solutions, without affecting the effective substances, even at such a high ionic strength (μ) of 0.2-0.4. Consequently, aminated PMLG spheres with a certain pore size are considered to be a useful adsorbent for the removal of endotoxin from drugs and fluids for injection.