- 著者
-
Harshita Sharma
Fumihito Ohtani
Parmila Kumari
Deepti Diwan
Naoko Ohara
Tetsuya Kobayashi
Miho Suzuki
Naoto Nemoto
Yoshibumi Matsushima
Koichi Nishigaki
- 出版者
- 日本生物物理学会
- 雑誌
- BIOPHYSICS (ISSN:13492942)
- 巻号頁・発行日
- vol.10, pp.55-62, 2014 (Released:2014-09-06)
- 参考文献数
- 32
- 被引用文献数
-
1
Familial clustering without any prerequisite knowledge becomes often necessary in Behavioral Science, and forensic studies in case of great disasters like Tsunami and earthquake requiring body-identification without any usable information. However, there has been no well-established method for this purpose although conventional ones such as short tandem repeats (STR) and single nucleotide polymorphism (SNP), which might be applied with toil and moil to some extent. In this situation, we could find that the universal genome distance-measuring method genome profiling (GP), which is made up of three elemental techniques; random PCR, micro-temperature gradient gel electrophoresis (μTGGE), and computer processing for normalization, can do this purpose with ease when applied to mouse families. We also confirmed that the sequencing approach based on the ccgf (commonly conserved genetic fragment appearing in the genome profile) was not completely discriminative in this case. This is the first demonstration that the familial clustering can be attained without a priori sequence information to the level of discriminating strains and sibling relationships. This method can complement the conventional approaches in preliminary familial clustering.