1 0 0 0 OA One-step triplex PCR/RT-PCR to detect canine distemper virus, canine parvovirus and canine kobuvirus
- 著者
- Dafei LIU Fei LIU Dongchun GUO Xiaoliang HU Zhijie LI Zhigang LI Jianzhang MA Chunguo LIU
- 出版者
- JAPANESE SOCIETY OF VETERINARY SCIENCE
- 雑誌
- Journal of Veterinary Medical Science (ISSN:09167250)
- 巻号頁・発行日
- vol.81, no.7, pp.1040-1042, 2019 (Released:2019-07-19)
- 参考文献数
- 21
- 被引用文献数
- 1 6
To rapidly distinguish Canine distemper virus (CDV), canine parvovirus (CPV), and canine kobuvirus (CaKoV) in practice, a one-step multiplex PCR/RT-PCR assay was developed, with detection limits of 102.1 TCID50 for CDV, 101.9 TCID50 for CPV and 103 copies for CaKoV. This method did not amplify nonspecific DNA or RNA from other canine viruses. Therefore, the assay provides a sensitive tool for the rapid clinical detection and epidemiological surveillance of CDV, CPV and CaKoV in dogs.
- 著者
- Dafei LIU Fei LIU Dongchun GUO Xiaoliang HU Zhijie LI Zhigang LI Jianzhang MA Chunguo LIU
- 出版者
- JAPANESE SOCIETY OF VETERINARY SCIENCE
- 雑誌
- Journal of Veterinary Medical Science (ISSN:09167250)
- 巻号頁・発行日
- pp.17-0442, (Released:2018-01-23)
- 被引用文献数
- 6
To rapidly distinguish Canine distemper virus (CDV), canine parvovirus (CPV), and canine kobuvirus (CaKoV) in practice, a one–step multiplex PCR/RT–PCR assay was developed, with detection limits of 102.1 TCID50 for CDV, 101.9 TCID50 for CPV and 103 copies for CaKoV. This method did not amplify nonspecific DNA or RNA from other canine viruses. Therefore, the assay provides a sensitive tool for the rapid clinical detection and epidemiological surveillance of CDV, CPV and CaKoV in dogs.