1 0 0 0 OA One-step triplex PCR/RT-PCR to detect canine distemper virus, canine parvovirus and canine kobuvirus
- 著者
- Dafei LIU Fei LIU Dongchun GUO Xiaoliang HU Zhijie LI Zhigang LI Jianzhang MA Chunguo LIU
- 出版者
- JAPANESE SOCIETY OF VETERINARY SCIENCE
- 雑誌
- Journal of Veterinary Medical Science (ISSN:09167250)
- 巻号頁・発行日
- vol.81, no.7, pp.1040-1042, 2019 (Released:2019-07-19)
- 参考文献数
- 21
- 被引用文献数
- 1 6
To rapidly distinguish Canine distemper virus (CDV), canine parvovirus (CPV), and canine kobuvirus (CaKoV) in practice, a one-step multiplex PCR/RT-PCR assay was developed, with detection limits of 102.1 TCID50 for CDV, 101.9 TCID50 for CPV and 103 copies for CaKoV. This method did not amplify nonspecific DNA or RNA from other canine viruses. Therefore, the assay provides a sensitive tool for the rapid clinical detection and epidemiological surveillance of CDV, CPV and CaKoV in dogs.
1 0 0 0 OA First report of feline bocavirus associated with severe enteritis of cat in Northeast China, 2015
- 著者
- Chunguo LIU Fei LIU Zhigang LI Liandong QU Dafei LIU
- 出版者
- JAPANESE SOCIETY OF VETERINARY SCIENCE
- 雑誌
- Journal of Veterinary Medical Science (ISSN:09167250)
- 巻号頁・発行日
- pp.17-0444, (Released:2018-02-20)
- 被引用文献数
- 17
Feline bocavirus (FBoV) is a newly identified bocavirus of cats in the family Parvoviridae. A novel FBoV HRB2015–LDF was first identified from the cat with severe enteritis in Northeast China, with an overall positive rate of 2.78% (1/36). Phylogenetic and homologous analysis of the complete genome showed that FBoV HRB2015–LDF was clustered into the FBoV branch and closely related to other FBoVs, with 68.7–97.5% identities. And the genes of VP1, NPA and NS1 shared 70.7–97.6%, 72.4–98.6%, and 67.2–98.0% nucleotide identities with other FBoVs, respectively. The results suggested that the FBoVs could be divided into two distinct lineages, and the difference of nucleotide identities was >20–30% between the lineages.
- 著者
- Dafei LIU Fei LIU Dongchun GUO Xiaoliang HU Zhijie LI Zhigang LI Jianzhang MA Chunguo LIU
- 出版者
- JAPANESE SOCIETY OF VETERINARY SCIENCE
- 雑誌
- Journal of Veterinary Medical Science (ISSN:09167250)
- 巻号頁・発行日
- pp.17-0442, (Released:2018-01-23)
- 被引用文献数
- 6
To rapidly distinguish Canine distemper virus (CDV), canine parvovirus (CPV), and canine kobuvirus (CaKoV) in practice, a one–step multiplex PCR/RT–PCR assay was developed, with detection limits of 102.1 TCID50 for CDV, 101.9 TCID50 for CPV and 103 copies for CaKoV. This method did not amplify nonspecific DNA or RNA from other canine viruses. Therefore, the assay provides a sensitive tool for the rapid clinical detection and epidemiological surveillance of CDV, CPV and CaKoV in dogs.