- 著者
- Md. Shafiullah Parvej Md Ashraful Alam Mio Shono Mst. Nusrat Zahan Mst. Misrat Masuma Parvez Wahedul Karim Ansari Md. Sayduzzaman Jowel Mohammad Sharif Uddin Eriko Kage-Nakadai Md. Tanvir Rahman Yoshikazu Nishikawa
- 出版者
- National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee
- 雑誌
- Japanese Journal of Infectious Diseases (ISSN:13446304)
- 巻号頁・発行日
- pp.JJID.2019.016, (Released:2019-08-30)
- 参考文献数
- 35
- 被引用文献数
- 4
Using multiplex real-time PCR, 960 fecal samples collected from poultry, cattle, and diarrheic patients in Bangladesh were screened for diarrheagenic Escherichia coli (DEC). Invasion-related gene virB had the highest prevalence in human patients (41%) and was shown to be positively correlated first with afaB for diffuse adhesion and second with aggR for aggregative adhesion. These three genes were specific to patients. In contrast, Shiga toxin genes stx1 (57%) and stx2 (40%) were prevalent in cattle samples. eae for attaching and effacing lesion, and elt and est of enterotoxins were detected from all three sample sources. Heat map and hierarchical clustering assigned the samples into five different clusters with patient samples positive for virB and afaB placing together in one cluster. Although detection of virulence genes cannot be a direct indication of the distribution of DEC organisms, their detection suggests that Shigella spp. or enteroinvasive E. coli are the most prevalent among diarrheagenic bacteria in Bangladesh and that diffusely adherent E. coli are concomitant bacterium. eae-possessing organisms in patients may come from cattle and poultry sources. The small number of stx-positive patients could be explained by the small number of animal samples that were positive for both eae and stx.
- 著者
- Md Shafiullah Parvej Md Ashraful Alam Mio Shono Mst Nusrat Zahan Mst Misrat Masuma Parvez Wahedul Karim Ansari Md Sayduzzaman Jowel Md Sharif Uddin Eriko Kage-Nakadai Md Tanvir Rahman Yoshikazu Nishikawa
- 出版者
- National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee
- 雑誌
- Japanese Journal of Infectious Diseases (ISSN:13446304)
- 巻号頁・発行日
- vol.73, no.1, pp.76-82, 2020 (Released:2020-01-23)
- 参考文献数
- 35
- 被引用文献数
- 4
Using multiplex real-time PCR, 960 fecal samples collected from poultry, cattle, and patients with diarrhea in Bangladesh were screened for diarrheagenic Escherichia coli (DEC). The invasion-related gene virB showed the highest prevalence in human patients (41%) and was shown to be positively correlated first with afaB with regards to diffuse adhesion and second with aggR with regards to aggregative adhesion. These three genes were specific to human patients. In contrast, the Shiga toxin genes stx1 (57%) and stx2 (40%) were prevalent in cattle samples. The eae gene, which is associated with attaching and effacing lesion formation, and the elt and est genes, which are associated with enterotoxins, were detected from all three sample sources. Heat map construction and hierarchical clustering assigned the samples into five different clusters, with the patient samples positive for virB and afaB being placed together in one cluster. Although the detection of virulence genes cannot be a direct indication of the distribution of diarrheagenic organisms, their detection suggests that Shigella spp. or enteroinvasive E. coli are the most prevalent diarrheagenic bacteria in Bangladesh and that diffusely adherent E. coli is concomitantly present with these bacteria. eae-possessing organisms in patients may come from cattle and poultry sources. The small number of stx-positive patients could be explained by the small number of animal samples that were positive for both eae and stx.