著者

Yue Meng Guang Yang Ai Ren Yunhe Qu Xue Wang Kevin H. Mayo Yifa Zhou Chengcheng Song

出版者

Japanese Society for Food Science and Technology

雑誌

Food Science and Technology Research (ISSN:13446606)

巻号頁・発行日

pp.FSTR-D-23-00039, (Released:2023-09-07)

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A 3-O-methylated-galactan WCCP-N-b from Cantharellus cibarius polarizes RAW264.7 macrophages, bone marrow-derived macrophages (BMDMs) and peritoneal macrophages (PEMs) leading to enhanced secretion of cytokine TNF-α and subsequent cytotoxic effects on HCT116 and CT26 colon cancer cells. Investigation into the mechanism of action suggests that WCCP-N-b polarizes macrophages by targeting Toll-like receptor 2 and activation of MAPKs and NF-κB signaling pathways. Overall, our results indicate that this linear 3-O-methylated-galactan inhibits colon cancer cells via conversion of M0 to M1 macrophages.

著者

Ping Ji Changmai Chen Yanan Hu Zixuan Zhan Wei Pan Rongrong Li Erguang Li Hui-Ming Ge Guang Yang

出版者

The Pharmaceutical Society of Japan

雑誌

Biological and Pharmaceutical Bulletin (ISSN:09186158)

巻号頁・発行日

vol.38, no.1, pp.1-6, 2015-01-01 (Released:2015-01-01)

参考文献数

21

被引用文献数

13 32

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The bark, leaves, and flowers of Paulownia trees have been used in traditional Chinese medicine to treat infectious and inflammatory diseases. We investigated the antiviral effects of Paulownia tomentosa flowers, an herbal medicine used in some provinces of P. R. China for the treatment of skin rashes and blisters. Dried flowers of P. tomentosa were extracted with methanol and tested for antiviral activity against enterovirus 71 (EV71) and coxsackievirus A16 (CAV16), the predominant etiologic agents of hand, foot, and mouth disease in P. R. China. The extract inhibited EV71 infection, although no effect was detected against CAV16 infection. Bioactivity-guided fractionation was performed to identify apigenin as an active component of the flowers. The EC50 value for apigenin to block EV71 infection was 11.0 µM, with a selectivity index of approximately 9.3. Although it is a common dietary flavonoid, only apigenin, and not similar compounds like naringenin and quercetin, were active against EV71 infection. As an RNA virus, the genome of EV71 has an internal ribosome entry site that interacts with heterogeneous nuclear ribonucleoproteins (hnRNPs) and regulates viral translation. Cross-linking followed by immunoprecipitation and reverse transcription-polymerase chain reaction (RT-PCR) analysis showed that EV71 RNA was associated with hnRNPs A1 and A2. Apigenin treatment disrupted this association, indicating that apigenin suppressed EV71 replication through a novel mechanism by targeting the trans-acting factors. This study therefore validates the effects of Paulownia against EV71 infection. It also yielded mechanistic insights on apigenin as an active compound for the antiviral activity of P. tomentosa against EV71 infection.