- 著者
- Megumi Matsui Haruka Kono Makoto Ogata
- 出版者
- The Japanese Society of Applied Glycoscience
- 雑誌
- Journal of Applied Glycoscience (ISSN:13447882)
- 巻号頁・発行日
- vol.65, no.3, pp.31-36, 2018-08-20 (Released:2018-08-20)
- 参考文献数
- 20
- 被引用文献数
- 5
A novel substrate {Galβ1,4GlcNAcβ1,4GlcNAc-β-pNP [Gal(GlcNAc)2-β-pNP]} for assaying lysozyme activity has been designed using docking simulations and enzymatic synthesis via β-1,4-galactosyltransferase-mediated transglycosylation from UDP-Gal as the donor to (GlcNAc)2-β-pNP as the acceptor. Hydrolysis of the synthesized Gal(GlcNAc)2-β-pNP and related compounds using hen egg-white lysozyme (HEWL) demonstrated that the substrate was specifically cleaved to Gal(GlcNAc)2 and p-nitrophenol (pNP). A combination of kinetic studies and docking simulation was further conducted to elucidate the mode of substrate binding. The results demonstrate that Gal(GlcNAc)2-β-pNP selectively binds to a subsite of lysozyme to liberate the Gal(GlcNAc)2 and pNP products. The work therefore describes a new colorimetric method for quantifying lysozyme on the basis of the determination of pNP liberated from the substrate.
- 著者
- Taichi KATO Chia-Te CHIU Die-Hua TSAI Elena PAVLENKO Marina KOSAREVA Hironobu ITOU Haruka KONO
- 出版者
- The Ornithological Society of Japan
- 雑誌
- ORNITHOLOGICAL SCIENCE (ISSN:13470558)
- 巻号頁・発行日
- vol.22, no.1, pp.97-104, 2023 (Released:2023-01-26)
- 参考文献数
- 29
We describe, for the first time, the rattling call of the Crested Honey Buzzard Pernis ptilorhynchus. This was previously considered to be a unique vocalization of European Honey Buzzard Pernis apivorus; however, we detected this series of rapidly repeated notes given by an adult female, a second-year, a juvenile and a nestling Crested Honey Buzzard. The rattling calls appeared to be given at times of great excitement, such as to an approaching intruder, mate or parent at the nest, or to a familiar person who usually gave food in the case of captive individuals. The rattling call of an adult female Crested Honey Buzzard at the nest was at a frequency of 2.12 (s.d.±0.07) kHz, and lasted 141 (s.d.±18) ms, which was higher and longer than in European Honey Buzzard.