- 著者
- Junqin Zhang Yuxi Ge Heng Zhang Zi Wang Weiqiang Dou Shudong Hu
- 出版者
- Japanese Society for Magnetic Resonance in Medicine
- 雑誌
- Magnetic Resonance in Medical Sciences (ISSN:13473182)
- 巻号頁・発行日
- pp.mp.2021-0067, (Released:2021-08-21)
- 参考文献数
- 24
- 被引用文献数
- 4
Purpose: Mucinous adenocarcinoma (MA) is associated with worse clinicopathological characteristics and a poorer prognosis than non-MA. Moreover, MA is related to worse tumor regression grade and tumor downstaging than non-MA. This study investigated whether lesions in MA and non-MA can be quantitatively assessed by T2 mapping technique and compared with the diffusion-weighted imaging (DWI).Methods: High-resolution MRI, DWI, and T2 mapping were performed on 81 patients diagnosed with rectal cancer via biopsy. Afterward, T2 and apparent diffusion coefficient (ADC) values were manually measured by a senior and a junior radiologist independently. By examining surgical specimens, the patients with MA and non-MA were identified. Inter-observer reproducibility was tested, and T2 and ADC values were compared using Mann–Whitney U test. Finally, receiver operating characteristic (ROC) curves were drawn to determine the cut-off value.Results: Of the 81 patients, 11 patients with MA were confirmed by pathology. The inter-observer reproducibility of T2 and ADC values showed an excellent intraclass correlation coefficient (ICC) of 0.993 and 0.913, respectively. MA had higher T2 (87.9 ± 5.11 ms) (P = 0.000) and ADC (2.03 × 10−3 mm2/s) (P = 0.000) values than non-MA (66.6 ± 6.86 ms and 1.17 × 10−3 mm2/s, respectively). The area under the ROC curves (AUC) of the T2 and ADC values were 0.999 (95% confidence interval [CI]: 0.953–1) and 0.979 (95% CI: 0.920–0.998), respectively. When the cutoff value in T2 mapping was 80 ms, the Youden index was the largest, sensitivity was 100%, and specificity was 97%.Conclusion: As a stable quantitative sequence, T2 mapping of MRI is useful in differentiating MA from non-MA. Compared to ADC values, T2 values are also diagnostically effective and non-inferior to ADC values.
- 著者
- Qing He Chuanfei Yu Lan Wang Yongbo Ni Heng Zhang Ying Du Hua Gao Junzhi Wang
- 出版者
- National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee
- 雑誌
- Japanese Journal of Infectious Diseases (ISSN:13446304)
- 巻号頁・発行日
- pp.JJID.2019.163, (Released:2019-10-31)
- 参考文献数
- 27
- 被引用文献数
- 3
Fever is a systemic inflammatory response of the body to pyrogens. Nuclear factor κB (NF-κB) is a central signalling molecule that causes the excessive secretion of various proinflammatory factors induced by pyrogens. This study explored the feasibility of a novel reporter gene assay (RGA) for pyrogen detection using RAW264.7 cells stably transfected with the NF-κB reporter gene as a pyrogenic marker. The RGA could detect different types of pyrogens, including the lipopolysaccharide of gram-negative bacteria, the lipoteichoic acid of gram-positive bacteria, and the zymosan of fungi, and a good dose-effect relationship was observed in terms of NF-κB activity. The limits of detection of the RGA to those pyrogens were 0.03 EU/ml, 0.001 μg/ml, and 1μg/ml, respectively. The method had good precision and accuracy and could be applied to many products [e.g., nivolumab, rituximab, bevacizumab, etanercept, basiliximab, Haemophilus influenza type b conjugate vaccine, 23-valent pneumococcal polysaccharide vaccine, group A and group C meningococcal conjugate vaccine, diphtheria, tetanus, pertussis (acellular, component), poliomyelitis (inactivated) vaccine, and imject alum adjuvant]. The results of this study suggest that the novel RGA has a wide pyrogen detection spectrum and is sufficiently sensitive, stable, and accurate for various applications.