著者

Yu Inoue Seiji Hasegawa Yuichi Hasebe Mika Kawagishi-Hotta Ryosuke Okuno Takaaki Yamada Hiroaki Adachi Katsuma Miyachi Yoshie Ishii Kazumitsu Sugiura Hirohiko Akamatsu

出版者

The Pharmaceutical Society of Japan

雑誌

Biological and Pharmaceutical Bulletin (ISSN:09186158)

巻号頁・発行日

vol.44, no.10, pp.1403-1412, 2021-10-01 (Released:2021-10-01)

参考文献数

29

被引用文献数

6

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Currently, human-skin derived cell culture is a basic technique essential for dermatological research, cellular engineering research, drug development, and cosmetic development. But the number of donors is limited, and primary cell function reduces through cell passage. In particular, since adult stem cells are present in a small amount in living tissues, it has been difficult to obtain a large amount of stem cells and to stably culture them. In this study, skin derived cells were isolated from the epidermis, dermis, and adipose tissue collected from single donor, and immortalization was induced through gene transfer. Subsequently, cell lines that could be used as stem cell models were selected using the differentiation potential and the expression of stem cell markers as indices, and it was confirmed that these could be stably cultured. The immortalized cell lines established in this study have the potential to be applied not only to basic dermatological research but also to a wide range of fields such as drug screening and cell engineering.

著者

足立 広明 Hiroaki Adachi

出版者

関学西洋史研究会

雑誌

関学西洋史論集 (ISSN:03860043)

巻号頁・発行日

vol.40, pp.29-46, 2017-03

著者

Keiko Ohnari Kazumasa Okada Osamu Higuchi Hidenori Matsuo Hiroaki Adachi

出版者

The Japanese Society of Internal Medicine

雑誌

Internal Medicine (ISSN:09182918)

巻号頁・発行日

vol.57, no.20, pp.3021-3024, 2018-10-15 (Released:2018-10-15)

参考文献数

17

被引用文献数

1 6

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An 82-year-old woman developed neck weakness and dysarthria with antibodies against acetylcholine receptor (AChR) and low-density lipoprotein receptor-related protein 4 (LRP4). Myasthenia gravis (MG) was diagnosed by edrophonium and repetitive nerve stimulation tests. Her symptoms resolved completely by immunotherapy. One year later, she presented with muscle weakness and bulbar palsy accompanied by atrophy and fasciculation. Her tendon reflexes were brisk, and Babinski's sign was positive. She was diagnosed with probable amyotrophic lateral sclerosis (ALS). Immunotherapy did not improve her symptoms, and she ultimately died of respiratory failure. MG and ALS may share a pathophysiology, including anti-LRP4 antibodies at the neuromuscular junction.

著者

安達 博明 Hiroaki Adachi

雑誌

人文論究 (ISSN:02866773)

巻号頁・発行日

vol.54, no.2, pp.87-98, 2004-09-25