著者
Fumio WATANABE Shigeo TAKENAKA Hiromi KITTAKA-KATSURA Shuhei EBARA Emi MIYAMOTO
出版者
Center for Academic Publications Japan
雑誌
Journal of Nutritional Science and Vitaminology (ISSN:03014800)
巻号頁・発行日
vol.48, no.5, pp.325-331, 2002 (Released:2009-04-28)
参考文献数
35
被引用文献数
43 90 76

Substantial amounts of vitamin B12 were found in some edible algae (green and purple layers) and algal health food (chlorella and spirulina tablets) using the Lactobacillus delbrueckii subsp. lactis ATCC7830 microbiological assay method. Corrinoidcompounds were purified and characterized from these algae to clarify the chemical properties and bioavailability of the algal vitamin B12. True vitamin B12 is the predominate cobamide of green and purple layers and chlorella tablets. Feeding the purple layer to vitamin B12-deficient rats significantly improved the vitamin B12 status. The results suggest that algal vitamin B12 is a bioavailable source for mammals. Pseudovitamin B12 (an inactive corrinoid) predominated in the spirulina tablets, which are not suitable for use as a vitamin B12 source, especially for vegetarians.
著者
Hiromi KITTAKA-KATSURA Fumio WATANABE Yoshihisa NAKANO
出版者
Center for Academic Publications Japan
雑誌
Journal of Nutritional Science and Vitaminology (ISSN:03014800)
巻号頁・発行日
vol.50, no.6, pp.438-440, 2004 (Released:2009-04-28)
参考文献数
7
被引用文献数
8 11

Vitamin B12 contents of green (0046-0263 and 0, 125-0535μg/ 100g dry weight), blue (0.068-0.081 and 0.525-0.528μg/100g dry weight), red (0.061 and 0.663μg/100g dry weight), and black (0.104-0.859 and 0.305-1.20μg/100g dry weight) tea leaves were obtained by intrinsic factor-chemiluminescence and microbiological methods, respectively. Although vitamin Bit was found in all tea leaves tested by both assay methods, the higher values by the microbiological method were not due to occurrence of both deoxyribosides and deoxynucleotides (known as an alkali-resistant factor), but may have been due to that of inactive corrinoid compounds for mammals in the tea leaves.