著者

Mifumi KAWABE Yuta BABA Reo TAMAI Ryohei YAMAMOTO Masayuki KOMORI Takashi MORI Shigeo TAKENAKA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.14-0641, (Released:2015-04-04)

被引用文献数

1 11

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Malignant melanoma is one of the most common and aggressive tumors in the oral cavity of dog. The tumor has a poor prognosis, and methods for diagnosis and prediction of prognosis after treatment are required. Here, we examined metabolite profiling using gas chromatography-mass spectrometry (GC-MS) for development of a discriminant model for evaluation of prognosis. Metabolite profiles were evaluated in healthy and melanoma plasma samples using orthogonal projection to latent structure using discriminant analysis (OPLS-DA). Cases that were predicted to be healthy using the OPLS discriminant model had no advanced lesions after radiation therapy. These results indicate that metabolite profiling may be useful in diagnosis and prediction of prognosis of canine malignant melanoma.

著者

Fumio WATANABE Shigeo TAKENAKA Hiromi KITTAKA-KATSURA Shuhei EBARA Emi MIYAMOTO

出版者

Center for Academic Publications Japan

雑誌

Journal of Nutritional Science and Vitaminology (ISSN:03014800)

巻号頁・発行日

vol.48, no.5, pp.325-331, 2002 (Released:2009-04-28)

参考文献数

35

被引用文献数

43 90 76

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Substantial amounts of vitamin B12 were found in some edible algae (green and purple layers) and algal health food (chlorella and spirulina tablets) using the Lactobacillus delbrueckii subsp. lactis ATCC7830 microbiological assay method. Corrinoidcompounds were purified and characterized from these algae to clarify the chemical properties and bioavailability of the algal vitamin B12. True vitamin B12 is the predominate cobamide of green and purple layers and chlorella tablets. Feeding the purple layer to vitamin B12-deficient rats significantly improved the vitamin B12 status. The results suggest that algal vitamin B12 is a bioavailable source for mammals. Pseudovitamin B12 (an inactive corrinoid) predominated in the spirulina tablets, which are not suitable for use as a vitamin B12 source, especially for vegetarians.

著者

Fumio WATANABE Shigeo TAKENAKA Hiromi KATSURA Emi MIYAMOTO Katsuo ABE Yoshiyuki TAMURA Toshiyuki NAKATSUKA Yoshihisa NAKANO

出版者

(社)日本農芸化学会

雑誌

Bioscience, Biotechnology, and Biochemistry (ISSN:09168451)

巻号頁・発行日

vol.64, no.12, pp.2712-2715, 2000 (Released:2005-01-28)

参考文献数

12

被引用文献数

34

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The edible purple laver, Porphyra yezoensis, contained 51.49±1.51 μg of vitamin B12 compounds per 100 g dry weight of the laver (mean±SEM, n=4). A vitamin B12 compound was purified from the lyophilized purple laver and partially characterized. The silica gel 60 TLC and reversed-phase HPLC patterns of the purified pink-colored compound were identical to those of authentic vitamin B12, but not to those of vitamin B12 analogues inactive for humans.

著者

Reo TAMAI Masaru FURUYA Shingo HATOYA Hideo AKIYOSHI Ryohei YAMAMOTO Yoshiaki KOMORI Shin-ichi YOKOI Kenichiro TANI Yuji HIRANO Masayuki KOMORI Shigeo TAKENAKA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.14-0043, (Released:2014-08-13)

被引用文献数

3 15

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Canine lymphoma is a common cancer that has high rates of complete remission with combination chemotherapy. However, the duration of remission varies based on multiple factors, and there is a need to develop a method for early detection of recurrence. In this study, we compared the metabolites profiles in serum from 21 dogs with lymphoma and 13 healthy dogs using gas chromatography mass spectrometry (GC-MS). The lymphoma group was separated from the control group in an orthogonal projection to latent structure with discriminant analysis (OPLS-DA) plot using ions of m/z 100–600, indicating that the metabolites profiles in lymphoma cases differed from those in healthy dogs. The lymphoma group was also separated from the control group on OPLS-DA plot using 29 metabolites identified in all serum samples. Significant differences were found for 16 of these metabolites with higher levels in the lymphoma group for 15 of the metabolites and lower levels for inositol. An OPLS-DA plot showed separation of the lymphoma and healthy groups using these 16 metabolites only. These results indicate that metabolites profile with GC-MS may be a useful tool for detection of potential biomarker and diagnosis of canine lymphoma.

著者

Fumio WATANABE Joachi SCHWARZ Shigeo TAKENAKA Emi MIYAMOTO Noriharu OHISHI Esther NELLE Rahel HOCHSTRASSER Yukinori YABUTA

出版者

Center for Academic Publications Japan

雑誌

Journal of Nutritional Science and Vitaminology (ISSN:03014800)

巻号頁・発行日

vol.58, no.6, pp.438-441, 2012 (Released:2013-02-19)

参考文献数

9

被引用文献数

9 28

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This study determined the vitamin B12 content of six wild edible mushrooms which are consumed by European vegetarians. Zero or trace levels (0.01-0.09 μg/100 g dry weight) of vitamin B12 were determined in porcini mushrooms (Boletus spp.), parasol mushrooms (Macrolepiota procera), oyster mushrooms (Pleurotus ostreatus), and black morels (Morchella conica). By contrast, black trumpet (Craterellus cornucopioides) and golden chanterelle (Cantharellus cibarius) mushrooms contained considerable levels (1.09-2.65 μg/100 g dry weight) of vitamin B12. To determine whether C. cornucopioides or C. cibarius contained vitamin B12 or other corrinoid compounds that are inactive in humans, we purified a corrinoid compound using an immunoaffinity column and identified it as vitamin B12 based on LC/ESI-MS/MS chromatograms.

著者

Tetsuya HASEGAWA Maho SUMITA Yusuke HORITANI Reo TAMAI Katsuhiro TANAKA Masayuki KOMORI Shigeo TAKENAKA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.13-0520, (Released:2013-12-13)

被引用文献数

3 15

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Epilepsy is a common neurological disorder with seizures, but diagnostic approaches in veterinary clinics remain limited. Cerebrospinal fluid (CSF) is a body fluid used for diagnosis in veterinary medicine. In this study, we explored canine epilepsy diagnostic biomarkers using gas chromatography-mass spectrometry (GC-MS)-based metabolic profiling of CSF and multivariate data analysis. Profiles for subjects with idiopathic epilepsy differed significantly from those of healthy controls and subjects with symptomatic epilepsy. Among 60 identified metabolites, the levels of 20 differed significantly among the three groups. Glutamic acid was significantly increased in idiopathic epilepsy, and some metabolites including ascorbic acid were changed in both forms of epilepsy. These findings show that metabolic profiles of CSF differ between idiopathic and symptomatic epilepsy and that metabolites including glutamic acid and ascorbic acid in CSF may be useful for diagnosis of canine epilepsy.