著者
Hiromichi TAMADA Nahoko ADACHI Noritoshi KAWATE Toshio INABA Shingo HATOYA Tsutomu SAWADA
出版者
公益社団法人 日本獣医学会
雑誌
Journal of Veterinary Medical Science (ISSN:09167250)
巻号頁・発行日
pp.15-0520, (Released:2015-11-22)
被引用文献数
2

Factors involved in patency of uterine cervices in the bitch with pyometra remain to be clarified. This study examined relationship between patency and mRNA levels for inducible nitric oxide synthase (iNOS), cyclooxygenase (COX)-1, COX-2 and prostaglandin E synthase (PGES) in the uterine cervix of bitches with pyometra. Cervical patency was measured by inserting the stainless steel rods with different diameter into cervical canals. Levels of mRNA expression were determined by semi-quantitative reverse transcription-polymerase chain reaction. The cervical patency was positively correlated with mRNA levels for COX-2 and PGES, but not those for iNOS and COX-1. The results suggest that gene expression of COX-2 and PGES may be involved in the regulation of patency in the uterine cervix of bitches with pyometra.
著者
Hiromichi TAMADA Kohei TAKEMOTO Masato TOMINAGA Noritoshi KAWATE Masahiro TAKAHASHI Shingo HATOYA Satoshi MATSUYAMA Toshio INABA Tsutomu SAWADA
出版者
日本繁殖生物学会
雑誌
Journal of Reproduction and Development (ISSN:09168818)
巻号頁・発行日
pp.2015-079, (Released:2015-10-26)
被引用文献数
2 4

Gene expression of epidermal growth factor (EGF), transforming growth factor-α (TGF-α) and EGF receptor (EGF-R) and the localization of the corresponding proteins in the canine testis were studied. Levels of mRNA expressions were determined by semiquantitative reverse transcription polymerase chain reaction in the testes of the peripubertal (4–6 mo), young adult (3–4 y), advanced adult (7–8 y) and senescent (11–16 y) groups. The EGF-R mRNA level in the testes of the peripubertal group was significantly higher than those in the other groups, whereas there was no difference in EGF and TGF-α mRNA levels among groups. Immunohistochemical stainings for EGF, TGF-α and EGF-R in the testis revealed that immunoreactivity in the seminiferous epithelium and Sertoli cell was weak and nonspecific for the stage of spermatogenesis, and distinct staining was found in Leydig cells. These results suggest that the EGF family of growth factors may be involved in testicular maturation and function in the dog.