著者

Hiroto FUKUI Hiroshi SHIMODA Sho KADEKARU Chizuka HENMI Yumi UNE

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.21-0007, (Released:2021-04-08)

被引用文献数

3

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Twenty-three of 42 European rabbits (Oryctolagus cuniculus), belonging to the same rabbit colony, died in March 2020 (55% mortality) in Chiba prefecture, Japan. The disease course was extremely acute without indicators of death or hemorrhage. Necropsy revealed liver swelling, discoloration, cloudiness and fragility, and pulmonary edema. Histologically, severe hepatocellular necrosis (mainly peripheral) and intra-glomerular capillary hyalin thrombi were observed. On molecular-biological examination, reverse transcription polymerase chain reaction analysis of RNA from tissues detected a rabbit hemorrhagic disease virus, confirmed as a RHDV-2 VP60 fragment, which shared 99.42% nucleotide identity with the homologous fragment of RHDV-2 German isolate by nucleotide sequence analysis. This report shows the outbreak of rabbit hemorrhagic disease caused by RHDV-2, an emerging infectious disease, in Japan.

著者

Tsuyoshi Takara Kazuo Yamamoto Naoko Suzuki Shin-ichiro Iio Hayata Noguchi Toshihiro Kakinuma Asami Baba Wakana Yamada Masafumi Nagata Hiroshi Shimoda

出版者

Society for Glycative Stress Research

雑誌

Glycative Stress Research (ISSN:21883602)

巻号頁・発行日

vol.9, no.3, pp.158-169, 2022-10-20 (Released:2022-10-26)

参考文献数

52

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Background and objective: Plant-derived glucosylceramides (GlcCer) have been clinically reported to enhance skin barrier functions. GlcCer and ceramides (Cer) interact with immune-stimulating receptors on antigen-presenting cells, such as c-type lectin receptors and toll-like receptor 4. However, the clinical and immunological efficacies of orally ingested GlcCer remain unknown. Therefore, we herein conducted a clinical trial on the effects of rice-derived GlcCer (Oryza Ceramide®: OC) on cold symptoms, immune parameters, and SF-36 scores. Methods: OC (type PCD, 60 mg daily) containing 1.8 mg of GlcCer and 0.09 mg of Cer [t18:0/24:0] was used as the active sample. We enrolled 44 healthy Japanese individuals who are prone to catching colds and a low immune score. All subjects were randomly allocated to an active group (n = 22) or placebo group (n = 22). Capsules containing OC or the placebo were administered for 8 weeks. Cold symptom scores during the intervention were the primary outcome, while immune parameters and SF-36 scores measured after the 8-week intervention were the secondary outcomes. Results: Forty subjects completed the trial, and the per protocol set comprised 19 and 21 in the active and placebo groups, respectively. Regarding the primary outcome, cumulative days with cold symptoms, such as nasal congestion, throat irritation, a cough, headache, muscle pain, and diarrhea, was lower in the OC group. Among the secondary outcomes, physical functioning in SF-36 scores was improved in the OC group. Regarding immune parameters, a negative correlation was observed between changes in blood T-lymphocytes and cumulative days with cold symptoms. Laboratory tests revealed no abnormalities to suggest adverse effects of OC. Conclusions: OC consisting of GlcCer attenuated typical cold symptoms, such as nasal congestion, throat irritation, a cough, headache, muscle pain, and diarrhea, and improved physical conditions. Changes in T-lymphocytes may be one of the mechanisms by which OC ameliorates cold symptoms.

著者

Kango TATEMOTO Keita ISHIJIMA Yudai KURODA Milagros Virhuez MENDOZA Yusuke INOUE Eunsil PARK Hiroshi SHIMODA Yuko SATO Tadaki SUZUKI Kazuo SUZUKI Shigeru MORIKAWA Ken MAEDA

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.22-0236, (Released:2022-05-31)

被引用文献数

7

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The present study investigated severe fever with thrombocytopenia syndrome virus (SFTSV) infection in raccoons in Wakayama Prefecture from 2007 to 2019. To perform surveillance, an enzyme-linked immunosorbent assay (ELISA) was established, and the sensitivity and specificity of the ELISA were 100% in comparison with a 50% focus-reduction neutralization assay. Using the established ELISA, we performed serosurveillance of SFTSV infection in 2,299 raccoons in Tanabe region, Wakayama Prefecture from 2007 to 2019. The first anti-SFTSV-positive raccoon was captured in October 2009. The seroprevalence of SFTSV infection was <10% between April 2009 and March 2013, 23.9% between April 2013 and March 2014, 37.5% between April, 2014 and March 2015, and over 50% from April 2015. Next, we performed detection of SFTSV genes in sera of raccoons captured in Wakayama Prefecture after April 2013. The results indicated that 2.4% of raccoons were positive for SFTSV genes and that the frequency of SFTSV infection among raccoons between January and March (0.7%) was lower than that between April and June (3.4%). In addition, virus genes were detected from many specimens, including sera and feces of two raccoons, and viral antigens were detected in lymphoid cells in lymphoid follicles in the colon by immunohistochemical staining. In conclusion, SFTSV had recently invaded the area and had rapidly spread among wild animals. The first patient in this area was reported in June 2014, indicating that raccoons are good sentinels for assessing the risk of SFTSV in humans.

著者

Junko SUZUKI Yohei NISHIO Yuki KAMEO Yutaka TERADA Ryusei KUWATA Hiroshi SHIMODA Kazuo SUZUKI Ken MAEDA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.77, no.11, pp.1457-1463, 2015 (Released:2015-12-01)

参考文献数

28

被引用文献数

3 18

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In 2007–2008, a canine distemper virus (CDV) epidemic occurred among wild animals in Wakayama Prefecture, Japan, and many mammals, including the wild boar and deer, were infected. In this study, CDV prevalence among wild animals was surveyed before and after the epidemic. At first, an enzyme-linked immunosorbent assay (ELISA) with horseradish peroxidase-conjugated protein A/G was established to detect CDV antibodies in many mammalian species. This established ELISA was available for testing dogs, raccoons and raccoon dogs as well as virus-neutralization test. Next, a serological survey of wild mammalians was conducted, and it was indicated that many wild mammalians, particularly raccoons, were infected with CDV during the epidemic, but few were infected before and after the epidemic. On the other hand, many raccoon dogs died during the epidemic, but CDV remained prevalent in the remaining population, and a small epidemic occurred in raccoon dogs in 2012–2013. These results indicated that the epidemic of 2007–2008 may have been intensified by transmission to raccoons.

著者

Karla Cristine C. DOYSABAS Mami OBA Tomoki ISHIBASHI Hideki SHIBATA Hitoshi TAKEMAE Hiroshi SHIMODA Tetsuya MIZUTANI Atsuo IIDA Eiichi HONDO

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.20-0021, (Released:2020-02-12)

被引用文献数

2

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Adenosine 5’-triphosphate (ATP), the major energy currency of the cell, is involved in many cellular processes, including the viral life cycle, and can be used as an indicator of early signs of cytopathic effect (CPE). In this study, we demonstrated that CPE can be analyzed using an FRET-based ATP probe named ATP indicator based on Epsilon subunit for Analytical Measurements (ATeam). The results revealed that as early as 3 hr, the virus infected cells showed a significantly different Venus/cyan fluorescent protein (CFP) ratio compared to the mock-infected cells. The ATeam technology is therefore useful to determine the early signs of ATP-based CPE as early as 3 hr without morphology-based CPE by light microscopy, and enables high throughput determination of the presence of microorganisms in neglected samples stored in laboratories.

著者

Dung VAN NGUYEN Junko SUZUKI Shohei MINAMI Kenzo YONEMITSU Nao NAGATA Ryusei KUWATA Hiroshi SHIMODA Chien Kim VU Thuy Quoc TRUONG Ken MAEDA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.16-0394, (Released:2016-10-14)

被引用文献数

8

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Canine distemper virus (CDV) is one of the most serious pathogens found in many species of carnivores, including domestic dogs. In this study, hemagglutinin (H) genes were detected in five domestic Vietnamese dogs with diarrhea, and two CDVs were successfully isolated from dogs positive for H genes. The complete genome of one isolate, CDV/dog/HCM/33/140816, was determined. Phylogenetic analysis showed that all Vietnamese CDVs belonged to the Asia-1 genotype. In addition, the H proteins of Vietnamese CDV strains were the most homologous to those of Chinese CDVs (98.4% to 99.3% identity). These results indicated that the Asia-1 genotype of CDV was the predominant genotype circulating among the domestic dog population in Vietnam and that transboundary transmission of CDV has occurred between Vietnam and China.

著者

Hiroshi SHIMODA Seiji TAMARU Masahiro MORIMOTO Toshiharu HAYASHI Masayuki SHIMOJIMA Ken MAEDA

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.73, no.9, pp.1241-1242, 2011 (Released:2011-09-30)

参考文献数

11

被引用文献数

11 15

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A previous serosurvey of Japanese encephalitis virus (JEV) among dogs suggested that dogs are well suited for use as sentinels for assessing the risk of JEV transmission to humans. To examine the clinical symptoms and duration of anti-JEV antibodies in dogs, three dogs were experimentally challenged with JEV. All JEV-infected dogs did not show any clinical signs or abnormal blood tests, except for C-reactive protein. Virus-neutralization titers rapidly increased and were maintained until 70 days postinfection, and neither the virus nor the viral genome was detected in blood. Thus, since dogs live in close proximity to humans as companion animals, they are well suited for use as sentinels for surveying the human risk of JEV infection.

著者

Hiroto FUKUI Hiroshi SHIMODA Sho KADEKARU Chizuka HENMI Yumi UNE

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.83, no.5, pp.841-845, 2021 (Released:2021-05-17)

参考文献数

16

被引用文献数

3

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Twenty-three of 42 European rabbits (Oryctolagus cuniculus), belonging to the same rabbit colony, died in March 2020 (55% mortality) in Chiba prefecture, Japan. The disease course was extremely acute without indicators of death or hemorrhage. Necropsy revealed liver swelling, discoloration, cloudiness and fragility, and pulmonary edema. Histologically, severe hepatocellular necrosis (mainly peripheral) and intra-glomerular capillary hyalin thrombi were observed. On molecular-biological examination, reverse transcription polymerase chain reaction analysis of RNA from tissues detected a rabbit hemorrhagic disease virus, confirmed as a RHDV-2 VP60 fragment, which shared 99.42% nucleotide identity with the homologous fragment of RHDV-2 German isolate by nucleotide sequence analysis. This report shows the outbreak of rabbit hemorrhagic disease caused by RHDV-2, an emerging infectious disease, in Japan.

著者

Dung VAN NGUYEN Yukata TERADA Shohei MINAMI Kenzo YONEMITSU Nao NAGATA Thanh Dinh Ha LE Ryusei KUWATA Hiroshi SHIMODA Ken MAEDA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.16-0538, (Released:2016-11-14)

被引用文献数

16

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Canine coronavirus (CCoV) is an important pathogen that causes enteritis in dogs, but there is no information on CCoV infection in Vietnam. To examine the prevalence of CCoV infection among Vietnamese dogs, 201 serum samples were analyzed by virus-neutralization (VN) test. The results showed that antibody against CCoV-II was present in 87 dogs (43.3%). To detect genes of CCoV, fecal samples collected from 30 diarrheic and 50 healthy dogs were examinated by RT-PCR, confirming that 2 diarrheic dogs and 5 healthy dogs were positive for CCoV. Nucleotide sequences of N-terminal region of spike (S) gene indicated that CCoV strains were divided into two subgenotypes, CCoV-IIa and -IIb, respectively. Furthemore, we succeeded in isolating CCoV/dog/HCM47/2015, the isolate was plaque-purified three times, and 3′-terminal one-third of the genome was analyzed. Interestingly, the plaque-purified virus had a large deletion in ORF3abc and E genes (1,165 nt), and a short deletion in ORF7b gene (60 nt), suggesting that these regions are not necessary for in vitro replication of CCoV. Next, the antigenicity between the isolated CCoV-IIb and the other CCoV-IIa was compared by VN test, revealing that antigenicty of the isolated CCoV is equal or higher than that of the other CCoV. In summary, two subgenotypes of CCoV-II are spreading among Vietnamese dogs. The isolated virus with a large deletion after in vitro passage may be useful for the development of vaccine, owing to its antigenicity and efficient viral growth in vitro.

著者

Yuka HARA Junko SUZUKI Keita NOGUCHI Yutaka TERADA Hiroshi SHIMODA Takuya MIZUNO Ken MAEDA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.13-0003, (Released:2013-03-26)

被引用文献数

4 7

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Morbilliviruses use signaling lymphocyte activation molecule (SLAM) as a receptor for their entry to cells. In this study, a complete gene encoding SLAM of a domestic cat was identified. The identity of feline SLAM with canine one was 73%, and feline SLAM formed the same cluster with those of carnivores. Furthermore, feline cell expressing feline SLAM supported growth of canine distemper virus (CDV) as well as that expressing canine one. These results indicated that feline SLAM can function as a receptor for morbilliviruses, and our established feline cells that express feline SLAM might be useful for analysis of morbilliviruses originated from felids.