著者

Noriko Nakajima Satoru Hata Yuko Sato Minoru Tobiume Harutaka Katano Keiko Kaneko Noriyo Nagata Michiyo Kataoka Akira Ainai Hideki Hasegawa Masato Tashiro Makoto Kuroda Tamami Odai Nobuyuki Urasawa Tomoyoshi Ogino Hiroaki Hanaoka Masahide Watanabe Tetsutaro Sata

出版者

National Institute of Infectious Diseases

雑誌

Japanese Journal of Infectious Diseases (ISSN:13446304)

巻号頁・発行日

vol.63, no.1, pp.67-71, 2010-01-29 (Released:2022-03-31)

参考文献数

1

被引用文献数

2 57

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We report the pathological and virological findings of the first autopsy case of the 2009 pandemic influenza (A/H1N1pdm) virus infection in Japan. A man aged 33 years with chronic heart failure due to dilated cardiomyopathy, mild diabetes mellitus, atopic dermatitis, bronchial asthma, and obesity died of respiratory failure and multiple organ dysfunction syndrome. Macroscopic examination showed severe plumonary edema and microscopically the lung sections showed very early exudative-stage diffuse alveolar damage (DAD). Immunohistochemistry revealed proliferation of the influenza (A/H1N1pdm) virus in alveolar epithelial cells, some of which expressed SAα2-3Gal on the cell surface. Influenza (A/H1N1pdm) virus genomic RNA and mRNA were also detected in alveolar epithelial cells. Real-time PCR revealed 723 copies/cell in the left lower lung section from which the influenza (A/H1N1pdm) virus was isolated. Electron microscopic analysis revealed filamentous viral particles in the lung tissue. The concentrations of various cytokines/chemokines in the serum and the autopsied lung tissue were measured. IL-2R, IL-6, IL-8, IL-10, IFN-α, MCP-1, and MIG levels were elevated in both. These findings indicated a case of viral pneumonia caused by influenza (A/H1N1pdm) virus infection, showing characteristic pathological findings of the early stage of DAD.

著者

Kango TATEMOTO Keita ISHIJIMA Yudai KURODA Milagros Virhuez MENDOZA Yusuke INOUE Eunsil PARK Hiroshi SHIMODA Yuko SATO Tadaki SUZUKI Kazuo SUZUKI Shigeru MORIKAWA Ken MAEDA

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.22-0236, (Released:2022-05-31)

被引用文献数

7

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The present study investigated severe fever with thrombocytopenia syndrome virus (SFTSV) infection in raccoons in Wakayama Prefecture from 2007 to 2019. To perform surveillance, an enzyme-linked immunosorbent assay (ELISA) was established, and the sensitivity and specificity of the ELISA were 100% in comparison with a 50% focus-reduction neutralization assay. Using the established ELISA, we performed serosurveillance of SFTSV infection in 2,299 raccoons in Tanabe region, Wakayama Prefecture from 2007 to 2019. The first anti-SFTSV-positive raccoon was captured in October 2009. The seroprevalence of SFTSV infection was <10% between April 2009 and March 2013, 23.9% between April 2013 and March 2014, 37.5% between April, 2014 and March 2015, and over 50% from April 2015. Next, we performed detection of SFTSV genes in sera of raccoons captured in Wakayama Prefecture after April 2013. The results indicated that 2.4% of raccoons were positive for SFTSV genes and that the frequency of SFTSV infection among raccoons between January and March (0.7%) was lower than that between April and June (3.4%). In addition, virus genes were detected from many specimens, including sera and feces of two raccoons, and viral antigens were detected in lymphoid cells in lymphoid follicles in the colon by immunohistochemical staining. In conclusion, SFTSV had recently invaded the area and had rapidly spread among wild animals. The first patient in this area was reported in June 2014, indicating that raccoons are good sentinels for assessing the risk of SFTSV in humans.

著者

金丸 葉子 佐藤 裕子 初鹿 円美 小竹 佐知子 金丸 葉子 佐藤 裕子 初鹿 円美 小竹 佐知子 Yoko KANEMARU Yuko SATO Marumi HATSUSHIKA Sachiko ODAKE カネマル ヨウコ Kanemaru Yoko サトウ ユウコ Sato Yuko ハツシカ マルミ Hatsushika Marumi オダケ サチコ Odake Sachiko

出版者

山梨県立女子短期大学

雑誌

山梨県立女子短期大学紀要 (ISSN:03850331)

巻号頁・発行日

no.34, pp.153-203, 2001

被引用文献数

1