- 著者
- Hong Li Qiang Liu Ningfu Wang Yizhou Xu Lan Kang Yaqi Ren Gangjie Zhu
- 出版者
- The Japanese Circulation Society
- 雑誌
- Circulation Journal (ISSN:13469843)
- 巻号頁・発行日
- pp.CJ-17-1251, (Released:2018-07-10)
- 参考文献数
- 37
- 被引用文献数
- 18
Background:In a previous study, a low level of miR-126-3p in endothelial progenitor cells (EPCs) was linked to the outcome of ischemic cardiomyopathy (ICM) patients. However, it remains unclear whether transplantation with miR-126-3p-overexpressing EPCs (MO-EPCs) can improve the cardiac function of ICM animal models.Methods and Results:miR-126-3p overexpression by lentiviral vector significantly increased migration and tube-like structures of EPCs from ICM patients. MO-EPCs or non-modified EPCs (NM-EPCs) were transplanted into nude rats with ICM induced by coronary artery ligation. MO-EPC transplantation increased capillary density and EPC survival rate in myocardial tissues of nude rats. Cytokines were also assessed by antibody array and real-time RT-PCR. G-CSF, VEGF-A, IL-3, IL-10, IGF-1, angiogenin, HGF, TIMP-1 and TIMP-2 were upregulated, and IL-8, MCP-1, MCP-2, TNF-α, TNF-β and MIP-1β were downregulated after miR-126-3p overexpression in EPCs. The same results were obtained in infarction tissues of nude rats after MO-EPC transplantation. Eight weeks after MO-EPC transplantation, left ventricular function improved significantly with clearly decreased infarction size, increased anterior wall thickness, and inhibition of inflammation compared with the results for NM-EPC transplantation. However, MO-EPC transplantation showed no increase in survival time of nude rats with ICM during 8 weeks of observation.Conclusions:miR-126-3p can restore the biology of EPCs from ICM patients. Moreover, MO-EPC transplantation improves cardiac function effectively, representing a promising future treatment for ICM.
- 著者
- Hong LI Wenjun CAO Chen WANG Xinrui ZHU Guisheng LIAO Zhangqing HE
- 出版者
- The Institute of Electronics, Information and Communication Engineers
- 雑誌
- IEICE TRANSACTIONS on Fundamentals of Electronics, Communications and Computer Sciences (ISSN:09168508)
- 巻号頁・発行日
- vol.E106-A, no.10, pp.1311-1321, 2023-10-01
The configurable Ring oscillator Physical unclonable function (CRO PUF) is the newly proposed strong PUF based on classic RO PUF, which can generate exponential Challenge-Response Pairs (CRPs) and has good uniqueness and reliability. However, existing proposals have low hardware utilization and vulnerability to modeling attacks. In this paper, we propose a Novel Configurable Dual State (CDS) PUF with lower overhead and higher resistance to modeling attacks. This structure can be flexibly transformed into RO PUF and TERO PUF in the same topology according to the parity of the Hamming Weight (HW) of the challenge, which can achieve 100% utilization of the inverters and improve the efficiency of hardware utilization. A feedback obfuscation mechanism (FOM) is also proposed, which uses the stable count value of the ring oscillator in the PUF as the updated mask to confuse and hide the original challenge, significantly improving the effect of resisting modeling attacks. The proposed FOM-CDS PUF is analyzed by building a mathematical model and finally implemented on Xilinx Artix-7 FPGA, the test results show that the FOM-CDS PUF can effectively resist several popular modeling attack methods and the prediction accuracy is below 60%. Meanwhile it shows that the FOM-CDS PUF has good performance with uniformity, Bit Error Rate at different temperatures, Bit Error Rate at different voltages and uniqueness of 53.68%, 7.91%, 5.64% and 50.33% respectively.
- 著者
- Hong Li Qiang Liu Ningfu Wang Yizhou Xu Lan Kang Yaqi Ren Gangjie Zhu
- 出版者
- The Japanese Circulation Society
- 雑誌
- Circulation Journal (ISSN:13469843)
- 巻号頁・発行日
- vol.82, no.9, pp.2332-2341, 2018-08-24 (Released:2018-08-24)
- 参考文献数
- 37
- 被引用文献数
- 7 18
Background: In a previous study, a low level of miR-126-3p in endothelial progenitor cells (EPCs) was linked to the outcome of ischemic cardiomyopathy (ICM) patients. However, it remains unclear whether transplantation with miR-126-3p-overexpressing EPCs (MO-EPCs) can improve the cardiac function of ICM animal models. Methods and Results: miR-126-3p overexpression by lentiviral vector significantly increased migration and tube-like structures of EPCs from ICM patients. MO-EPCs or non-modified EPCs (NM-EPCs) were transplanted into nude rats with ICM induced by coronary artery ligation. MO-EPC transplantation increased capillary density and EPC survival rate in myocardial tissues of nude rats. Cytokines were also assessed by antibody array and real-time RT-PCR. G-CSF, VEGF-A, IL-3, IL-10, IGF-1, angiogenin, HGF, TIMP-1 and TIMP-2 were upregulated, and IL-8, MCP-1, MCP-2, TNF-α, TNF-β and MIP-1β were downregulated after miR-126-3p overexpression in EPCs. The same results were obtained in infarction tissues of nude rats after MO-EPC transplantation. Eight weeks after MO-EPC transplantation, left ventricular function improved significantly with clearly decreased infarction size, increased anterior wall thickness, and inhibition of inflammation compared with the results for NM-EPC transplantation. However, MO-EPC transplantation showed no increase in survival time of nude rats with ICM during 8 weeks of observation. Conclusions: miR-126-3p can restore the biology of EPCs from ICM patients. Moreover, MO-EPC transplantation improves cardiac function effectively, representing a promising future treatment for ICM.