- 著者
- Ikeda Kaori Fujimoto Shimpei Morling Beth Ayano-Takahara Shiho Carroll Andrew E. Harashima Shin-ichi Uchida Yukiko Inagaki Nobuya
- 出版者
- Public Library of Science
- 雑誌
- PLOS ONE (ISSN:19326203)
- 巻号頁・発行日
- vol.9, no.10, 2014-10-15
- 被引用文献数
- 10
糖尿病患者の心の負担に日本人特有の要因の存在 -協調性を重視する文化の影響-. 京都大学プレスリリース. 2014-10-16.
1 0 0 0 OA Noninvasive Monitoring of β-Cell Mass and Fetal β-Cell Genesis in Mice Using Bioluminescence Imaging
- 著者
- Sekiguchi Yukari Owada Junya Oishi Hisashi Katsumata Tokio Ikeda Kaori Kudo Takashi Takahashi Satoru
- 出版者
- Japanese Association for Laboratory Animal Science
- 雑誌
- Experimental animals (ISSN:13411357)
- 巻号頁・発行日
- vol.61, no.4, pp.445-451, 2012
- 被引用文献数
- 9 4
Bioluminescence imaging (BLI) has been applied in gene therapy and research to screen for transgene expression, progression of infection, tumor growth and metastasis, and transplantation. It enables real-time and relatively noninvasive localization and serial quantification of biological processes in experimental animals. In diabetes research, BLI has been employed for the quantification of β-cell mass, monitoring of islet graft survival after transplantation, and detection of reporter gene expression. Here, we explore the use of BLI in a transgenic mouse expressing luciferase under the control of the mouse insulin 1 promoter (MIP-Luc-VU). A previous report on MIP-Luc-VU mice showed luminescence intensities emitted from the islets correlated well with the number of islets in vitro and in vivo. In this study, we showed MIP-Luc-VU mice fed a high fat diet for 8 weeks gave rise to a greater bioluminescent signal than mice fed a regular diet for the same period of time. Conversely, there was a strong reduction in the signal observed in diabetic Mafa-deficient/Mafk-transgenic mutant mice and streptozotocin-treated mice, reflecting the loss of β-cells. Furthermore, we were able to monitor fetal β-cell genesis in MIP-Luc-VU mice during the late gestational stage in a noninvasive and repetitive manner. In summary, we show that bioluminescence imaging of mice expressing a β-cell specific reporter allows detection of changes in β-cell mass and visualization of fetal β-cell neogenesis in uteri.