著者

Imanishi Tadashi Itoh Takeshi Suzuki Yutaka O'Donovan Claire Fukuchi Satoshi Koyanagi Kanako O. Barrero Roberto A. Tamura Takuro Yamaguchi-Kabata Yumi Tanino Motohiko Yura Kei Miyazaki Satoru Ikeo Kazuho Homma Keiichi Kasprzyk Arek Nishikawa Tetsuo Hirakawa Mika Thierry-Mieg Jean Thierry-Mieg Danielle Ashurst Jennifer Jia Libin Nakao Mitsuteru Thomas Michael A. Mulder Nicola Karavidopoulou Youla Jin Lihua Kim Sangsoo Yasuda Tomohiro Lenhard Boris Eveno Eric Suzuki Yoshiyuki Yamasaki Chisato Takeda Jun-ichi Gough Craig Hilton Phillip Fujii Yasuyuki Sakai Hiroaki Tanaka Susumu Amid Clara Bellgard Matthew Bonaldo Maria de Fatima Bono Hidemasa Bromberg Susan K. Brookes Anthony J. Bruford Elspeth Carninci Piero Chelala Claude Couillault Christine Souza Sandro J. de Debily Marie-Anne Devignes Marie-Dominique Dubchak Inna Endo Toshinori Estreicher Anne Eyras Eduardo Fukami-Kobayashi Kaoru R. Gopinath Gopal Graudens Esther Hahn Yoonsoo Han Michael Han Ze-Guang Hanada Kousuke Hanaoka Hideki Harada Erimi Hashimoto Katsuyuki Hinz Ursula Hirai Momoki Hishiki Teruyoshi Hopkinson Ian Imbeaud Sandrine Inoko Hidetoshi Kanapin Alexander Kaneko Yayoi Kasukawa Takeya Kelso Janet Kersey Paul Kikuno Reiko Kimura Kouichi Korn Bernhard Kuryshev Vladimir Makalowska Izabela Makino Takashi Mano Shuhei Mariage-Samson Regine Mashima Jun Matsuda Hideo Mewes Hans-Werner Minoshima Shinsei Nagai Keiichi Nagasaki Hideki Nagata Naoki Nigam Rajni Ogasawara Osamu Ohara Osamu Ohtsubo Masafumi Okada Norihiro Okido Toshihisa Oota Satoshi Ota Motonori Ota Toshio Otsuki Tetsuji Piatier-Tonneau Dominique Poustka Annemarie Ren Shuang-Xi Saitou Naruya Sakai Katsunaga Sakamoto Shigetaka Sakate Ryuichi Schupp Ingo Servant Florence Sherry Stephen Shiba Rie Shimizu Nobuyoshi Shimoyama Mary Simpson Andrew J. Soares Bento Steward Charles Suwa Makiko Suzuki Mami Takahashi Aiko Tamiya Gen Tanaka Hiroshi Taylor Todd Terwilliger Joseph D. Unneberg Per Veeramachaneni Vamsi Watanabe Shinya Wilming Laurens Yasuda Norikazu Yoo Hyang-Sook Stodolsky Marvin Makalowski Wojciech Go Mitiko Nakai Kenta Takagi Toshihisa Kanehisa Minoru Sakaki Yoshiyuki Quackenbush John Okazaki Yasushi Hayashizaki Yoshihide Hide Winston Chakraborty Ranajit Nishikawa Ken Sugawara Hideaki Tateno Yoshio Chen Zhu Oishi Michio Tonellato Peter Apweiler Rolf Okubo Kousaku Wagner Lukas Wiemann Stefan Strausberg Robert L. Isogai Takao Auffray Charles Nomura Nobuo Gojobori Takashi Sugano Sumio

出版者

Public Library of Science

雑誌

PLoS Biology (ISSN:15449173)

巻号頁・発行日

vol.2, no.6, pp.856-875, 2004-06

被引用文献数

3 226

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The human genome sequence defines our inherent biological potential; the realization of the biology encoded therein requires knowledge of the function of each gene. Currently, our knowledge in this area is still limited. Several lines of investigation have been used to elucidate the structure and function of the genes in the human genome. Even so, geneprediction remains a difficult task, as the varieties of transcripts of a gene may vary to a great extent. We thus performed an exhaustive integrative characterization of 41,118 full-length cDNAs that capture the gene transcripts as complete functional cassettes, providing an unequivocal report of structural and functional diversity at the gene level.Our international collaboration has validated 21,037 human gene candidates by analysis of high-quality full-length cDNA clones through curation using unified criteria. This led to the identification of 5,155 new gene candidates. It also manifested the most reliable way to control the quality of the cDNA clones. We have developed a human gene database, called the H-Invitational Database (H-InvDB; http://www.h-invitational.jp/). It provides the following:integrative annotation of human genes, description of gene structures, details of novel alternative splicing isoforms, non-protein-coding RNAs, functional domains, subcellular localizations, metabolic pathways, predictions of protein three-dimensional structure, mapping of known single nucleotide polymorphisms (SNPs), identification of polymorphic microsatellite repeats within human genes, and comparative results with mouse full-length cDNAs. The H-InvDB analysis has shown that up to 4% of the human genome sequence (National Center for Biotechnology Information build 34 assembly) may contain misassembled or missing regions. We found that 6.5% of the human gene candidates(1,377 loci) did not have a good protein-coding open reading frame, of which 296 loci are strong candidates for nonprotein-coding RNA genes. In addition, among 72,027 uniquely mapped SNPs and insertions/deletions localized within human genes, 13,215 nonsynonymous SNPs, 315 nonsense SNPs, and 452 indels occurred in coding regions. Together with 25 polymorphic microsatellite repeats present in coding regions, they may alter protein structure, causingphenotypic effects or resulting in disease. The H-InvDB platform represents a substantial contribution to resources needed for the exploration of human biology and pathology.

著者

Ikeda Kaori Fujimoto Shimpei Morling Beth Ayano-Takahara Shiho Carroll Andrew E. Harashima Shin-ichi Uchida Yukiko Inagaki Nobuya

出版者

Public Library of Science

雑誌

PLOS ONE (ISSN:19326203)

巻号頁・発行日

vol.9, no.10, 2014-10-15

被引用文献数

10

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糖尿病患者の心の負担に日本人特有の要因の存在 -協調性を重視する文化の影響-. 京都大学プレスリリース. 2014-10-16.

著者

Izutsu Minako Zhou Jun Sugiyama Yuzo Nishimura Osamu Aizu Tomoyuki Toyoda Atsushi Fujiyama Asao Agata Kiyokazu Fuse Naoyuki

出版者

Public Library of Science

雑誌

PLoS ONE (ISSN:19326203)

巻号頁・発行日

vol.7, no.3, pp.e33288, 2012-03-14

被引用文献数

28

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「暗黒ショウジョウバエ」のゲノム解読-暗闇への適応のメカニズム. 京都大学プレスリリース. 2012-03-15.

著者

Kim Young-il Hirai Shizuka Goto Tsuyoshi Ohyane Chie Takahashi Haruya Tsugane Taneaki Konishi Chiaki Fujii Takashi Inai Shuji Iijima Yoko Aoki Koh Shibata Daisuke Takahashi Nobuyuki Kawada Teruo

出版者

Public Library of Science

雑誌

PLoS ONE (ISSN:19326203)

巻号頁・発行日

vol.7, no.2, pp.e31317, 2012-02-09

被引用文献数

57

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トマトから脂肪肝、血中中性脂肪改善に有効な健康成分を発見:効果を肥満マウスで確認. 京都大学プレスリリース. 2012-02-10.

著者

Morisaka Tadamichi Sakai Mai Kogi Kazunobu Nakasuji Akane Sakakibara Kasumi Kasanuki Yuria Yoshioka Motoi

出版者

Public Library of Science

雑誌

PloS one (ISSN:19326203)

巻号頁・発行日

vol.8, no.8, 2013-08-28

被引用文献数

4

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野生イルカの突発性射精(夢精)を世界で初めて記録 : さまざまな動物で見られる現象か?. 京都大学プレスリリース. 2013-08-28 Spontaneous ejaculation, which is defined as the release of seminal fluids without apparent sexual stimulation, has been documented in boreoeutherian mammals. Here we report spontaneous ejaculation in a wild Indo-Pacific bottlenose dolphin (Tursiops aduncus), and present a video of this rare behavior. This is the first report of spontaneous ejaculation by an aquatic mammal, and the first video of this behavior in animals to be published in a scientific journal.

著者

Outani Hidetatsu Okada Minoru Yamashita Akihiro Nakagawa Kanako Yoshikawa Hideki Tsumaki Noriyuki

出版者

Public Library of Science

雑誌

PLoS ONE (ISSN:19326203)

巻号頁・発行日

vol.8, no.10, 2013-10-16

被引用文献数

75

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ヒトの皮膚細胞から軟骨様細胞へ直接変換に成功. 京都大学プレスリリース. 2013-10-14.

著者

Tanaka Akihito Woltjen Knut Miyake Katsuya Hotta Akitsu Ikeya Makoto Yamamoto Takuya Nishino Tokiko Shoji Emi Sehara-Fujisawa Atsuko Manabe Yasuko Fujii Nobuharu Hanaoka Kazunori Era Takumi Yamashita Satoshi Isobe Ken-ichi Kimura En Sakurai Hidetoshi

出版者

Public Library of Science

雑誌

PLoS ONE (ISSN:19326203)

巻号頁・発行日

vol.8, no.4, 2013-04-23

被引用文献数

156

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効率よく、再現性高くヒトiPS細胞から筋肉細胞を作製 -筋肉疾患の創薬プラットフォームの開発に向けて-. 京都大学プレスリリース. 2013-04-24.

著者

Shimizu Tokurou Kitajima Akira Nonaka Keisuke Yoshioka Terutaka Ohta Satoshi Goto Shingo Toyoda Atsushi Fujiyama Asao Mochizuki Takako Nagasaki Hideki Kaminuma Eli Nakamura Yasukazu

出版者

Public Library of Science

雑誌

PLOS ONE (ISSN:19326203)

巻号頁・発行日

vol.11, no.11, 2016-11-30

被引用文献数

76

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ミカンの親はどの品種? : 遺伝解析により60種以上のカンキツ類の親子関係が明らかに. 京都大学プレスリリース. 2017-01-13.

著者

Nishimura Takeshi Mori Futoshi Hanida Sho Kumahata Kiyoshi Ishikawa Shigeru Samarat Kaouthar Miyabe-Nishiwaki Takako Hayashi Misato Tomonaga Masaki Suzuki Juri Matsuzawa Tetsuro Matsuzawa Teruo

出版者

Public Library of Science

雑誌

PLOS computational biology (ISSN:15537358)

巻号頁・発行日

vol.12, no.3, 2016-03-24

被引用文献数

24

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ヒトの鼻腔機能はチンパンジーに及ばない -それでも、原人は出アフリカを遂げた-. 京都大学プレスリリース. 2016-03-25.

著者

Masuda Reiji Hatakeyama Makoto Yokoyama Katsuhide Tanaka Masaru

出版者

Public Library of Science

雑誌

PLOS ONE (ISSN:19326203)

巻号頁・発行日

vol.11, no.12, 2016-12-12

被引用文献数

18

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5年間の潜水調査で2011年津波後の海の生き物たちの回復が明らかに. 京都大学プレスリリース. 2016-12-13.

著者

Tanabe Akifumi S. Toju Hirokazu

出版者

Public Library of Science

雑誌

PLoS ONE (ISSN:19326203)

巻号頁・発行日

vol.8, no.10, 2013-10-18

被引用文献数

208

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あらゆる生物の名前をDNAに基づいて特定する「DNAバーコーディング」の理論的枠組みを確立. 京都大学プレスリリース. 2013-10-19.

著者

Yanai Goichi Hayashi Takashi Zhi Qi Yang Kai-Chiang Shirouzu Yasumasa Shimabukuro Takashi Hiura Akihito Inoue Kazutomo Sumi Shoichiro

出版者

Public Library of Science

雑誌

PLoS ONE (ISSN:19326203)

巻号頁・発行日

vol.8, no.5, 2013-05-28

被引用文献数

31

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膵島細胞と間葉系幹細胞の融合細胞を用いた糖尿病治療実験に成功 -新しい重症糖尿病治療法の開発に期待-. 京都大学プレスリリース. 2013-05-29.

著者

Narusaka Mari Kubo Yasuyuki Hatakeyama Katsunori Imamura Jun Ezura Hiroshi Nanasato Yoshihiko Tabei Yutaka Takano Yoshitaka

出版者

Public Library of Science

雑誌

PLoS ONE (ISSN:19326203)

巻号頁・発行日

vol.8, no.2, pp.e55954, 2013-02

被引用文献数

77 10

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A major class of disease resistance (R) genes which encode nucleotide binding and leucine rich repeat (NB-LRR) proteins have been used in traditional breeding programs for crop protection. However, it has been difficult to functionally transfer NB-LRR-type R genes in taxonomically distinct families. Here we demonstrate that a pair of Arabidopsis (Brassicaceae) NB-LRR-type R genes, RPS4 and RRS1, properly function in two other Brassicaceae, Brassica rapa and Brassica napus, but also in two Solanaceae, Nicotiana benthamiana and tomato (Solanum lycopersicum). The solanaceous plants transformed with RPS4/RRS1 confer bacterial effector-specific immunity responses. Furthermore, RPS4 and RRS1, which confer resistance to a fungal pathogen Colletotrichum higginsianum in Brassicaceae, also protect against Colletotrichum orbiculare in cucumber (Cucurbitaceae). Importantly, RPS4/RRS1 transgenic plants show no autoimmune phenotypes, indicating that the NB-LRR proteins are tightly regulated. The successful transfer of two R genes at the family level implies that the downstream components of R genes are highly conserved. The functional interfamily transfer of R genes can be a powerful strategy for providing resistance to a broad range of pathogens.

著者

Fujita Mari Sasanuma Hiroyuki Yamamoto Kimiyo N. Harada Hiroshi Kurosawa Aya Adachi Noritaka Omura Masato Hiraoka Masahiro Takeda Shunichi Hirota Kouji

出版者

Public Library of Science

雑誌

PLoS ONE (ISSN:19326203)

巻号頁・発行日

vol.8, no.4, 2013-04-03

被引用文献数

19

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DNAが切れていないのに発生する染色体断裂の発見 -ヒトの被爆線量を測定する手法に異議あり-. 京都大学プレスリリース. 2013-04-04.

著者

Yamamoto Shinya Humle Tatyana Tanaka Masayuki

出版者

Public Library of Science

雑誌

PLoS ONE (ISSN:19326203)

巻号頁・発行日

vol.8, no.1, 2013-01-30

被引用文献数

56

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技を盗むチンパンジー : 効率のよい道具使用テクニックを他者から見て学ぶ--ヒト特有と考えられている累積進化文化の認知的基盤--. 京都大学プレスリリース. 2013-01-31.

著者

Cheng Li-Tao Nagata Shogo Hirano Kunio Yamaguchi Shinpei Horie Shigeo Ainscough Justin Tada Takashi

出版者

Public Library of Science

雑誌

PLoS ONE (ISSN:19326203)

巻号頁・発行日

vol.7, no.2, pp.e32018, 2012-02-09

被引用文献数

10

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マウスiPS細胞を用いた遺伝性疾患の治療モデル-多発性嚢胞腎(ADPKD)モデルマウスの治療-. 京都大学プレスリリース. 2012-2-10.

著者

Fujikawa Keiko Iwata Takeshi Inoue Kaoru Akahori Masakazu Kadotani Hanako Fukaya Masahiro Watanabe Masahiko Chang Qing Barnett Edward M. Swat Wojciech

出版者

Public Library of Science

雑誌

PLoS ONE (ISSN:19326203)

巻号頁・発行日

vol.5, no.2, pp.e9050, 2010-02-04

被引用文献数

42

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Background: Glaucoma is a leading cause of blindness worldwide. Nonetheless, the mechanism of its pathogenesis has not been well-elucidated, particularly at the molecular level, because of insufficient availability of experimental genetic animal models. Methodology/Principal Findings: Here we demonstrate that deficiency of Vav2 and Vav3, guanine nucleotides exchange factors for Rho guanosine triphosphatases, leads to an ocular phenotype similar to human glaucoma. Vav2/Vav3-deficient mice, and to a lesser degree Vav2-deficient mice, show early onset of iridocorneal angle changes and elevated intraocular pressure, with subsequent selective loss of retinal ganglion cells and optic nerve head cupping, which are the hallmarks of glaucoma. The expression of Vav2 and Vav3 tissues was demonstrated in the iridocorneal angle and retina in both mouse and human eyes. In addition, a genome-wide association study screening glaucoma susceptibility loci using single nucleotide polymorphisms analysis identified VAV2 and VAV3 as candidates for associated genes in Japanese open-angle glaucoma patients. Conclusions/Significance: Vav2/Vav3-deficient mice should serve not only as a useful murine model of spontaneous glaucoma, but may also provide a valuable tool in understanding of the pathogenesis of glaucoma in humans, particularly the determinants of altered aqueous outflow and subsequent elevated intraocular pressure.

著者

Takei Yutaka Sakaguchi Eiji Sasaki Koichi Tomoyasu Yoko Yamamoto Kouji Yasuda Yasuharu

出版者

Public Library of Science

雑誌

PLOS ONE

巻号頁・発行日

vol.17, no.9, pp.e0274604, 2022-09-14

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Transporting patients down stairs by carrying is associated with a particularly high fall risk for patients and the occurrence of back pain among emergency medical technicians. The present study aimed to verify the effectiveness of the Airstretcher device, which was developed to reduce rescuers’ physical burden when transporting patients by dragging along the floor and down stairs. Forty-one paramedical students used three devices to transport a 65-kg manikin down stairs from the 3rd to the 1st floor. To verify the physical burden while carrying the stretchers, ratings of perceived exertion were measured using the Borg CR10 scale immediately after the task. Mean Borg CR10 scores (standard deviation) were 3.6 (1.7), 4.1 (1.8), 5.6 (2.4), and 4.2 (1.8) for the Airstretcher with dragging, Airstretcher with lifting, backboard with lifting, and tarpaulin with lifting conditions, respectively (p < 0.01). Multiple comparisons revealed that the Airstretcher with dragging condition was associated with significantly lower Borg CR10 scores compared with the backboard with lifting condition (p < 0.01). When the analysis was divided by handling position, estimated Borg CR10 values (standard error) for head position were 4.4 (1.3), 2.9 (0.9), 3.2 (0.8), and 4.0 (1.1) for the Airstretcher with dragging, Airstretcher with lifting, backboard with lifting, and tarpaulin with lifting conditions, respectively, after adjusting for participant and duration time (F = 1.4, p < 0.25). The estimated Borg CR10 value (standard error) for toe position in the Airstretcher with dragging condition was 2.0 (0.8), and the scores for the side position were 4.9 (0.4), 6.1 (0.3), and 4.7 (0.4) for the Airstretcher with lifting, backboard with lifting, and tarpaulin with lifting conditions, respectively, after adjusting for participant and duration time (F = 3.6, p = 0.02). Transferring a patient down stairs inside a house by dragging using the Airstretcher may reduce the physical burden for rescuers.

著者

清水 徳朗 北島 宣 野中 圭介 吉岡 照高 太田 智 後藤 新悟 豊田 敦 藤山 秋佐夫 望月 孝子 長崎 秀樹 神沼 英里 中村 保一

出版者

Public Library of Science

雑誌

PLOS ONE (ISSN:19326203)

巻号頁・発行日

vol.11, no.11, 2016-11-30

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ミカンの親はどの品種? : 遺伝解析により60種以上のカンキツ類の親子関係が明らかに. 京都大学プレスリリース. 2017-01-13.

著者

末盛 博文 Suemori Hirofumi Hirai Yuko Hamasaki Kanya Kodama Yoshiaki Mitani Hiroshi Landes Reid D. Nakamura Nori

出版者

Public Library of Science

雑誌

PLOS ONE (ISSN:19326203)

巻号頁・発行日

vol.10, no.8, 2015-08-21

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It is becoming clear that apparently normal somatic cells accumulate mutations. Such accumulations or propagations of mutant cells are thought to be related to certain diseases such as cancer. To better understand the nature of somatic mutations, we developed a mouse model that enables in vivo detection of rare genetically altered cells via GFP positive cells. The mouse model carries a partial duplication of 3' portion of X-chromosomal HPRT gene and a GFP gene at the end of the last exon. In addition, although HPRT gene expression was thought ubiquitous, the expression level was found insufficient in vivo to make the revertant cells detectable by GFP positivity. To overcome the problem, we replaced the natural HPRT-gene promoter with a CAG promoter. In such animals, termed HPRT-dup-GFP mouse, losing one duplicated segment by crossover between the two sister chromatids or within a single molecule of DNA reactivates gene function, producing hybrid HPRT-GFP proteins which, in turn, cause the revertant cells to be detected as GFP-positive cells in various tissues. Frequencies of green mutant cells were measured using fixed and frozen sections (liver and pancreas), fixed whole mount (small intestine), or by means of flow cytometry (unfixed splenocytes). The results showed that the frequencies varied extensively among individuals as well as among tissues. X-ray exposure (3 Gy) increased the frequency moderately (~2 times) in the liver and small intestine. Further, in two animals out of 278 examined, some solid tissues showed too many GFP-positive cells to score (termed extreme jackpot mutation). Present results illustrated a complex nature of somatic mutations occurring in vivo. While the HPRT-dup-GFP mouse may have a potential for detecting tissue-specific environmental mutagens, large inter-individual variations of mutant cell frequency cause the results unstable and hence have to be reduced. This future challenge will likely involve lowering the background mutation frequency, thus reducing inter-individual variation.