- 著者
- Shigeru Hanamata Jumpei Sawada Bunki Toh Seijiro Ono Kazunori Ogawa Togo Fukunaga Ken-Ichi Nonomura Takamitsu Kurusu Kazuyuki Kuchitsu
- 出版者
- Japanese Society for Plant Cell and Molecular Biology
- 雑誌
- Plant Biotechnology (ISSN:13424580)
- 巻号頁・発行日
- vol.36, no.2, pp.99-105, 2019-06-25 (Released:2019-07-03)
- 参考文献数
- 35
- 被引用文献数
- 10
We have previously shown that autophagy is required for post meiotic anther development including programmed cell death-mediated degradation of the tapetum and pollen maturation in rice. However, the spatiotemporal dynamics of autophagy in the tapetum remain poorly understood. We here established an in vivo imaging technique to analyze the dynamics of autophagy in rice tapetum cells by expressing green fluorescent protein-tagged AtATG8, a marker for autophagosomes. 3D-imaging analysis revealed that the number of autophagosomes/autophagy-related structures is extremely low at the tetrad stage (stage 8), and autophagy is dramatically induced at the uninucleate stages (stage 9–10) throughout the tapetal cells during anther development. The present monitoring system for autophagy offers a powerful tool to analyze the regulation of autophagy in rice tapetal cells during pollen maturation.
- 著者
- Toshinori MORISAKU Hitomi ONUKI Kenji HASHIMOTO Kazuyuki KUCHITSU Hiroharu YUI
- 出版者
- The Japan Society for Analytical Chemistry
- 雑誌
- Analytical Sciences (ISSN:09106340)
- 巻号頁・発行日
- vol.35, no.11, pp.1203-1207, 2019-11-10 (Released:2019-11-10)
- 参考文献数
- 21
- 被引用文献数
- 2
A near-infrared laser-induced surface deformation (NIR-LISD) microscope is developed and is applied to the dynamic viscoelastic measurements of the surface of a living plant cell. In the microscope, the deformation of the surface is induced by an NIR laser beam, and then the change in intensity of the probe beam reflected from the surface reflects its viscoelasticity. The application of the NIR laser beam has a great advantage for the prevention of damage to the plant cell compared to the irradiation of a visible laser beam in LISD measurements. The NIR-LISD microscope allows for discriminating the differences in power spectra between the subapical and lateral regions of single rhizoids. It is a useful method for the dynamic viscoelastic measurements of cells, such as plant cells, that are damaged due to the strong absorption of ultraviolet or visible light.