著者

清末 知宏 鎌田 博 原田 宏

出版者

Japanese Society for Plant Cell and Molecular Biology

雑誌

植物組織培養 (ISSN:02895773)

巻号頁・発行日

vol.6, no.3, pp.162-164, 1989 (Released:2010-04-30)

参考文献数

11

被引用文献数

26 33

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ニンジン (Daucus carota L. cv. US春蒔五寸) 実生の頂芽を含む組織片を高濃度 (0.1-0.4M) の塩化ナトリウムを含むMS培地で培養した後, これを含まない Murashige & Skoog 培地に移植・培養することで, 植物ホルモンの添加無しに, 体細胞から不定胚形成を行わせることに成功した.

著者

鳥山 欽哉 土屋 亨

出版者

Japanese Society for Plant Cell and Molecular Biology

雑誌

植物組織培養 (ISSN:02895773)

巻号頁・発行日

vol.9, no.2, pp.128-130, 1992 (Released:2010-04-30)

参考文献数

5

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Tobacco was transformed with the firefly luciferase gene. Once the naked eye became accustomed to the dark, light emission could be seen from the transgenic callus and the regenerated plants. A simple method for taking a picture of the light emission using instant film is described.

著者

Shigeru Hanamata Jumpei Sawada Bunki Toh Seijiro Ono Kazunori Ogawa Togo Fukunaga Ken-Ichi Nonomura Takamitsu Kurusu Kazuyuki Kuchitsu

出版者

Japanese Society for Plant Cell and Molecular Biology

雑誌

Plant Biotechnology (ISSN:13424580)

巻号頁・発行日

vol.36, no.2, pp.99-105, 2019-06-25 (Released:2019-07-03)

参考文献数

35

被引用文献数

10

---

We have previously shown that autophagy is required for post meiotic anther development including programmed cell death-mediated degradation of the tapetum and pollen maturation in rice. However, the spatiotemporal dynamics of autophagy in the tapetum remain poorly understood. We here established an in vivo imaging technique to analyze the dynamics of autophagy in rice tapetum cells by expressing green fluorescent protein-tagged AtATG8, a marker for autophagosomes. 3D-imaging analysis revealed that the number of autophagosomes/autophagy-related structures is extremely low at the tetrad stage (stage 8), and autophagy is dramatically induced at the uninucleate stages (stage 9–10) throughout the tapetal cells during anther development. The present monitoring system for autophagy offers a powerful tool to analyze the regulation of autophagy in rice tapetal cells during pollen maturation.

著者

Bernardo Gutiérrez María Mercedes Cobo Miguel Orellana Joely Vega Venancio Arahana Viviana Jaramillo María de Lourdes Torres

出版者

Japanese Society for Plant Cell and Molecular Biology

雑誌

Plant Biotechnology (ISSN:13424580)

巻号頁・発行日

vol.36, no.2, pp.91-97, 2019-06-25 (Released:2019-07-03)

参考文献数

25

---

The development of in vitro propagation methods can improve the current commercial use and conservation of plants like naranjilla (Solanum quitoense), a distinctive Andean crop and key emerging agricultural product. In the present study, we report in vitro culture protocols for naranjilla apical buds, hypocotyls and petioles. In apical bud culture, MS medium supplemented with 0.10 mg l−1 1-naphtaleneacetic acid (NAA) produced longer plantlets with greater number of leaves. Hypocotyl culture yielded higher number of shoots when using older explants in MS medium supplemented with different combinations of NAA, 6-benzylaminopurine (BAP) and gibberellic acid (GA3). Petiole culture produced a significantly higher number of shoots per explant, with more abundant and bigger leaves, when using MS medium supplemented with 0.02 mg l−1 NAA, 4.50 mg l−1 BAP and 1.00 mg l−1 GA3. A factorial analysis reveals that the interaction between GA3 and NAA/BAP plays an important role in shoot regeneration. These results provide new tools for the in vitro regeneration of naranjilla plants, improving on previously reported protocols for this species by using alternative explant types and regeneration protocols.

著者

Kinuyo Yoneya Junji Takabayashi

出版者

Japanese Society for Plant Cell and Molecular Biology

雑誌

Plant Biotechnology (ISSN:13424580)

巻号頁・発行日

vol.31, no.5, pp.409-416, 2014-12-25 (Released:2015-02-27)

参考文献数

55

被引用文献数

5 42

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When exposed to herbivore-infested plant volatiles or volatiles from artificially damaged plants, intact plants enhance their defense against herbivores. This phenomenon is called plant-plant communication. Here, we outline studies on plant-plant communication from both ecological and plant physiological perspectives. Regarding the ecological perspective, we give an overview of studies showing that plant–plant communication affect direct and indirect defense levels of exposed plants, and herbivore performance on exposed plants. Cases of kin selection in plant–plant communications and intra-plant communication via airborne signals are also summarized. Regarding the plant physiological perspective, we give an overview of studies that showed specific responses of receiver plants to a volatile molecular species, to different configurations of a volatile molecular species and to blends of volatiles. Furthermore, we review the signaling pathways involved, priming, sensitivity, and how plants receive volatile compounds in plant–plant communications.

著者

Tian Tian Tan Taku Demura Misato Ohtani

出版者

Japanese Society for Plant Cell and Molecular Biology

雑誌

Plant Biotechnology (ISSN:13424580)

巻号頁・発行日

vol.36, no.1, pp.1-6, 2019-03-25 (Released:2019-03-31)

参考文献数

45

被引用文献数

10

---

Xylem is an essential conductive tissue in vascular plants, and secondary cell wall polymers found in xylem vessel elements, such as cellulose, hemicellulose, and lignin, are promising sustainable bioresources. Thus, understanding the molecular mechanisms underlying xylem vessel element differentiation is an important step towards increasing woody biomass and crop yields. Establishing in vitro induction systems, in which vessel element differentiation is induced by phytohormonal stimuli or by overexpression of specific transcription factors, has been vital to this research. In this review, we present an overview of these in vitro induction systems, and describe two recently developed in vitro induction systems, VISUAL (Vascular cell Induction culture System Using Arabidopsis Leaves) and the KDB system. Furthermore, we discuss the potentials and limitations of each of these new in vitro induction systems for advancing our understanding of the molecular mechanisms driving xylem vessel element differentiation.

著者

Ngoc-Ha Thi Tran Taichi Oguchi Etsuko Matsunaga Akiyoshi Kawaoka Kazuo N. Watanabe Akira Kikuchi

出版者

Japanese Society for Plant Cell and Molecular Biology

雑誌

Plant Biotechnology (ISSN:13424580)

巻号頁・発行日

pp.18.0831a, (Released:2018-12-14)

参考文献数

33

被引用文献数

7

---

Under the Japanese biosafety regulatory framework for transgenic plants, data for assessing a transgenic plant’s impact on biodiversity must be submitted in order to obtain approval for a confined field trial. We recently reported the development of four novel transgenic Eucalyptus camaldulensis clones expressing the bacterial choline oxidase A (codA) gene, i.e., codAH-1, codAH-2, codAN-1, and codAN-2, and evaluated their abiotic tolerance by semiconfined screen house trial cultivation. Here we evaluated the impacts of the transgenic E. camaldulensis clones on productivities of harmful substances from those clones to affect soil microorganisms and/or other plants in the environment. A comparison of the assessment data between the transgenic trees and non-transgenic comparators showed no significant difference in potential impacts on biodiversity. The results contribute to sound-science evidence ensuring substantial equivalence between transgenic and non-transgenic E. camaldulensis.

著者

織田 弥三郎 澤田 裕樹

出版者

Japanese Society for Plant Cell and Molecular Biology

雑誌

植物組織培養 (ISSN:02895773)

巻号頁・発行日

vol.7, no.3, pp.164-169, 1990 (Released:2010-04-30)

参考文献数

16

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組織培養による食用ウチワサボテン (Opuntia ficus-indica) の大量増殖法を検討し, 以下の知見を得た.1. 基本培地としては検討した4種類の既成培地のうちMS培地が最も適していた.2. 増殖培地では, BA 1mg/l, NAA 0~0.1mg/lの植物生長調節物質の組合せでシュートの増殖が良好であった.3. また, 増殖培地中のゲル化剤に関しては寒天0.4%またはゲルライト0.2~0.3%が適していた.4. 発根培地において, NAAを5mg/l添加することにより発根および地上部の生長とも増加した.5. 発根した小植物体の馴化は容易であり, 生長にともなって親植物と同様の形質を示した.

著者

鈴木 正彦

出版者

Japanese Society for Plant Cell and Molecular Biology

雑誌

植物組織培養 (ISSN:02895773)

巻号頁・発行日

vol.1, no.1, pp.30, 1984 (Released:2010-04-30)

参考文献数

2

著者

Simon Vial-Pradel Yoshinori Hasegawa Ayami Nakagawa Shido Miyaki Yasunori Machida Shoko Kojima Chiyoko Machida Hiro Takahashi

出版者

Japanese Society for Plant Cell and Molecular Biology

雑誌

Plant Biotechnology (ISSN:13424580)

巻号頁・発行日

vol.36, no.4, pp.213-222, 2019-12-25 (Released:2019-12-27)

参考文献数

34

被引用文献数

2

---

DNA methylation in higher organisms has become an expanding field of study as it often involves the regulation of gene expression. Although Whole Genome Bisulfite Sequencing (WG-BS) based on next-generation sequencing (NGS) is the most versatile method, this is a costly technique that lacks in-depth analytic power. There are no conventional methods based on NGS that enable researchers to easily compare the level of DNA methylation from the practical number of samples handled in the laboratory. Although the targeted BS method based on Sanger sequencing is generally used in this case, it lacks in-depth analytic power. Therefore, we propose a new method that combines the high throughput analytic power of NGS and bioinformatics with the specificity and focus offered by PCR-amplification-based bisulfite sequencing methods. We use in silico size sieving of DNA-fragments and primer matchings instead of whole-fragment alignment in our bioinformatics analyses, and named our method SIMON (Simple Inference for Methylome based On NGS). The results of our targeted BS method based on NGS (SIMON method) show that small variations in DNA methylation patterns can be precisely and efficiently measured at a single nucleotide resolution. SIMON method combines pre-existing techniques to provide a cost-effective technique for in-depth studies that focus on pre-identified loci. It offers significant improvements with regard to workflow and the quality of the acquired DNA methylation information. Because of the high accuracy of the analysis, small variations of DNA methylation levels can be precisely determined even with large numbers of samples and loci.

著者

Masaomi Yamamura Soichiro Noda Takefumi Hattori Amiu Shino Jun Kikuchi Keiji Takabe Shiro Suzuki Daisuke Shibata Toshiaki Umezawa

出版者

Japanese Society for Plant Cell and Molecular Biology

雑誌

Plant Biotechnology (ISSN:13424580)

巻号頁・発行日

pp.12.0618a, (Released:2012-09-15)

参考文献数

53

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This article has been retracted at the request of the authors and under the agreement between the Editor-in-chief, Taku Demura and the authors.

著者

古谷 力 折原 裕 高木 さつき 吉田 淑子

出版者

Japanese Society for Plant Cell and Molecular Biology

雑誌

植物組織培養 (ISSN:02895773)

巻号頁・発行日

vol.5, no.2, pp.82-86, 1988 (Released:2010-04-30)

参考文献数

11

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奥多摩産ワサビ (Wsabia japonica Matsum.) の根茎より6種の分化段階の異なる培養組織を得た. これらの sinigrin 含量, myrosinase 活性を比較し, 分化と辛味発現の関係を考察した. Myrosinase 活性は脱分化したカルスから幼苗にいたるまですべての培養株に認められたが, sinigrin は少なくとも幼根・子葉様組織を併せ持つまで分化が進まなければ検出できなかった. さらに分化段階が進むにつれ sinigrin 含量は増加した. ワサビ原植物では sinigrin は全草に認められるが, 特に根茎部に多い. このてとから分化段階の進行による sinigrin 含量の増加は根茎部の肥大によるところが大きいと考えられる.

著者

喜久田 嘉郎 斉藤 渉 岡澤 養三

出版者

Japanese Society for Plant Cell and Molecular Biology

雑誌

植物組織培養 (ISSN:02895773)

巻号頁・発行日

vol.1, no.1, pp.18-21, 1984 (Released:2010-04-30)

参考文献数

8

被引用文献数

2 5

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ジャガイモ (品種メイクイン薯) を用いて, 高い生存率と分裂活性をもつ葉肉プロトプラストを無菌茎葉培養より単離・培養する方法を開発した。単離したプロトプラストは適当な培地で暗所25℃に培養すると, 48時間以内に細胞壁を再生し, 72~96時間後にDNA合成を開始した。その後, 細胞分裂を開始しカルスを形成した。細胞分裂の開始誘導には0.5mg/lのNAAと1.0mg/lのゼアチンが最も効果的であった。形成したカルスを0.1mg/lIAAと1.0mg/lのゼアチンを含む適当な培地に照度4,000lux, 20℃で培養すると茎葉分化が認められた。

著者

福井 宏至

出版者

Japanese Society for Plant Cell and Molecular Biology

雑誌

植物組織培養 (ISSN:02895773)

巻号頁・発行日

vol.1, no.1, pp.14-17, 1984 (Released:2010-04-30)

参考文献数

18

著者

高山 眞策

出版者

Japanese Society for Plant Cell and Molecular Biology

雑誌

植物組織培養 (ISSN:02895773)

巻号頁・発行日

vol.1, no.1, pp.8-13, 1984 (Released:2010-04-30)

参考文献数

100

被引用文献数

2

著者

大野 清春

出版者

Japanese Society for Plant Cell and Molecular Biology

雑誌

植物組織培養 (ISSN:02895773)

巻号頁・発行日

vol.1, no.1, pp.2-7, 1984 (Released:2010-04-30)

参考文献数

27

被引用文献数

1

著者

藤原 彰夫

出版者

Japanese Society for Plant Cell and Molecular Biology

雑誌

植物組織培養 (ISSN:02895773)

巻号頁・発行日

vol.1, no.1, pp.1, 1984 (Released:2010-04-30)

著者

Yoko Kamiya Fumitaka Abe Masafumi Mikami Masaki Endo Kanako Kawaura

出版者

Japanese Society for Plant Cell and Molecular Biology

雑誌

Plant Biotechnology (ISSN:13424580)

巻号頁・発行日

vol.37, no.2, pp.247-251, 2020-06-25 (Released:2020-06-25)

参考文献数

11

被引用文献数

12

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Genome editing using CRISPR/Cas9 is useful for common wheat because common wheat has allohexaploid nature and it can induce mutations simultaneously in three homoeologous genes. Although Agrobacterium-mediated transformation has advantages in genome editing, it still has low efficiency and requires relatively long time in wheat. Therefore, the use of guide RNAs (gRNAs) with efficient mutagenesis in vivo is one of the critical factors for producing genome-edited mutant lines in a short time. In this study, we targeted three genes in common wheat and established a rapid method for detection of mutations induced by the biolistic transient expression system. Biolistic transient expression of the gRNAs and Cas9 was achieved in immature wheat embryos. Mutations were detected a week later using PCR-RFLP and verified by the sequencing of genomic clones. We confirmed several types of mutations that occurred at different rates depending on the target sequences. Furthermore, frequencies of mutations tended to be higher at the targets that were edited at higher rates in the plants transformed by Agrobacterium. These results show that this method of rapid detection of edited mutations could be used for variety of applications, such as screening of target sequences or modified vectors for efficient CRISPR/Cas9 genome editing in wheat.

著者

Irene Ferreres Mirari Ortega Camilo López-Cristoffanini Salvador Nogués Xavier Serrat

出版者

Japanese Society for Plant Cell and Molecular Biology

雑誌

Plant Biotechnology (ISSN:13424580)

巻号頁・発行日

vol.36, no.4, pp.269-273, 2019-12-25 (Released:2019-12-27)

参考文献数

22

被引用文献数

5

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Anther culture is a fast tool to obtain double haploid plant lines for breeding purposes. In rice, this procedure is commonly performed in two steps: i) induction of calli from anthers and ii) regeneration of plantlets from calli. It has been stated that genotype highly influences the anther culture efficiency, so the media used in each step should be optimized for each variety. In this study, we tested different media modifications of an efficient protocol optimized for a medium sized grain temperate japonica NRVC980385, used as a control, in a long grain temperate japonica rice variety (NRVC20120346), and two long grain tropical japonica varieties (303012 and 303013).We found that the addition of 150 mg l−1 colchicine to the induction medium worked best for all genotypes except for NRVC20120346, whose best induction was obtained with the colchicine-free medium. Referring to regeneration, increased gelling agent in the medium provided the best rates in NRVC980385, improving our former NRVC980385-optimized anther culture protocol. Sorbitol fortified regeneration medium worked the best in the case of the long grain varieties. The presence of colchicine in the induction medium was also related to a higher obtention of double haploid plantlets. This study highlights that genotype is a key factor in the performance of rice anther culture. It has set a first anther culture study on long grain japonica varieties and optimizes the anther culture protocol for temperate japonica medium grain NRVC980385 with the use of colchicine and other additives that increase osmotic stress.

著者

Hiroyuki Kajiura Kyoko Hiwasa-Tanase Hiroshi Ezura Kazuhito Fujiyama

出版者

Japanese Society for Plant Cell and Molecular Biology

雑誌

Plant Biotechnology (ISSN:13424580)

巻号頁・発行日

vol.35, no.4, pp.375-379, 2018-12-25 (Released:2018-12-31)

参考文献数

17

被引用文献数

1 4

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Miraculin is a promising protein with taste-modifying properties. Focusing on the unique function and potential of miraculin, recombinant miraculin production has been explored with the use of heterologous expression systems, but the activities of recombinant miraculins were much lower than those of native miraculin, probably due to the difference in post-translational modification, especially N-glycosylation. For practical use therefore, the differences between N-glycan of recombinant miraculin compared to that of native miraculin should be minimized. Here, to establish the platform for functional miraculin production, we expressed miraculin in tomato plants with the same taste-modifying activity as native miraculin purified from miracle fruit, and we compared the N-glycan structures with those of native miraculin. Our N-glycan structural analysis using purified miraculin, followed by hydrazynolysis, 2-pyridylamine (PA)-labeling, high-performance liquid chromatography, and a liquid chromatography tandem-mass spectrometry analysis revealed that both the native and recombinant miraculins carried an M3 structure as a predominant structure and that most of the N-glycan structures on the miraculins were pauci-mannosidic structures with a smaller amount of plant-specific α1,3-fucosylated and/or β1,2-xylosylated N-glycans and without a Lewis a epitope. These results indicate that the N-glycoform of native miraculin from miracle fruit and recombinant miraculin expressed in tomato plants are almost identical to each other with similar ratios and that, therefore, plant-specific N-glycans are essential for showing the full taste-modifying activity of miraculin.