- 著者
- Takuma Yamamoto Katsuhiko Yoshizawa Shin-ichi Kubo Yuko Emoto Kenji Hara Brian Waters Takahiro Umehara Takehiko Murase Kazuya Ikematsu
- 出版者
- 日本毒性病理学会
- 雑誌
- Journal of Toxicologic Pathology (ISSN:09149198)
- 巻号頁・発行日
- vol.28, no.1, pp.33-36, 2015 (Released:2015-02-24)
- 参考文献数
- 17
- 被引用文献数
- 3 34
Caffeine (1,3,7-trimethylxanthine) is a popular mild central nervous system stimulant found in the leaves, seeds and fruits of various plants and in foodstuffs such as coffee, tea, and chocolate, among others. Caffeine is widely used and is not associated with severe side effects when consumed at relatively low doses. Although rarely observed, overdoses can occur. However, only a few fatal caffeine intoxication cases have been reported in the literature. Herein, we report the pathological examination results and information on caffeine concentrations in the blood, urine and main organs in a fatal caffeine intoxication case. Even though high caffeine concentrations were found in the systemic organs, no caffeine-related pathological changes were detected.
- 著者
- Brian Waters Kenji Hara Natsuki Ikematsu Mio Takayama Aya Matsusue Masayuki Kashiwagi Shin-ichi Kubo
- 出版者
- 日本法科学技術学会
- 雑誌
- 日本法科学技術学会誌 (ISSN:18801323)
- 巻号頁・発行日
- vol.23, no.2, pp.125-131, 2018 (Released:2018-07-30)
- 参考文献数
- 19
- 被引用文献数
- 1
The aim of this study was to develop a practical method to analyze tetrodotoxin (TTX), quantitatively, from postmortem specimens, not only blood and urine, but also organs. Extraction was achieved with 2% acetic acid and the use of an anion-exchange solid-phase extraction (SPE) cartridge. The quantitation method was a standard addition method with a calibration curve consisting of at least 3 points and an internal standard, voglibose (VOG). Separation by LC-MS/MS was achieved using a Luna HILIC (Phenomenex) column. The mobile phase was acetonitrile: 5 mM ammonium formate buffer (95:5), delivered at 0.2 mL/min. The selected reaction monitoring (SRM) transitions for TTX and VOG were m/z 320>302 and m/z 268>92, respectively. Cleaner extracts were achieved by using a lipid removal cartridge and washing with heptane. The addition of steps to remove interfering components that are prominent in postmortem samples aided in successful analysis. The HILIC column improved the retention of TTX to greater than 2 min to avoid the area where ion suppression has its greatest effect. Also, the use of anion-exchange SPE lessened the influence of acetic acid used during extraction. By using this method, we were able to quantitate low levels of TTX in postmortem specimens.