著者

Yuji Mizuno Seiji Hokimoto Eisaku Harada Kenji Kinoshita Michihiro Yoshimura Hirofumi Yasue

出版者

The Japanese Circulation Society

雑誌

Circulation Journal (ISSN:13469843)

巻号頁・発行日

pp.CJ-16-0969, (Released:2016-11-29)

参考文献数

36

被引用文献数

16 22

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Background:Coronary spastic angina (CSA) is common among East Asians and tobacco smoking (TS) is an established risk factor for CSA. Aldehyde dehydrogenase 2 (ALDH2) plays a key role in removing reactive toxic aldehydes and a deficient variant ALDH2 genotype (ALDH2*2) is prevalent among East Asians. We examined the interaction between TS andALDH2*2as a risk factor for CSA to better understand the disease pathogenesis.Methods and Results:The study subjects comprised 410 patients (258 men, 152 women; mean age, 66.3±11.5) in whom intracoronary injection of acetylcholine was performed on suspicion of CSA.ALDH2genotyping was performed by direct application of the Taqman polymerase chain reaction system. Of the study subjects, 244 had CSA proven and 166 were non-CSA. The frequencies of male sex,ALDH2*2, alcohol flushing syndrome, TS, coronary organic stenosis, and plasma levels of uric acid were higher (P<0.001, P<0.001, P<0.001, P<0.001, P<0.001, and P=0.015, respectively) and that of high-density lipoprotein cholesterol lower (P=0.002) in the CSA than non-CSA group. Multivariable logistic regression analysis revealed thatALDH2*2and TS were significant risk factors for CSA (P<0.001 and P=0.002, respectively).ALDH2*2exacerbated TS risk for CSA more than the multiplicative effects of each.Conclusions:ALDH2*2synergistically exacerbates TS risk for CSA, probably through aldehydes.

著者

競 和佳 今井 美穂 森次 美和子 山森 元博 村田 成範 木下 健司 Madoka Kisoi Miho Imai Miwako Moritsugu Motohiro Yamamori Shigenori Murata Kenji Kinoshita

雑誌

武庫川女子大学紀要. 自然科学編 (ISSN:09163123)

巻号頁・発行日

vol.65, pp.1-5, 2018-03-31

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Though drugstore pharmacists can also be known as primary health care pharmacists, it cannot yet be said that they completely fill this role. We believe that it is crucial for pharmacists to consult reliably with patients. To develop this ability, it is necessary to select a familiar and relevant theme by considering various factors that are worth discussing in their own right through conducting simple verification experiments. Caffeine is found in many foods and drinks, as well as in over the counter cold remedies and analgesics. Moreover, it has been widely used around the world to probe the effectiveness of drug-metabolizing enzyme activity of medications for liver disease. We have built a simple and easy experiment system to evaluate the pharmacokinetics of caffeine in saliva, and we examined the relationship between the drug-metabolizing enzyme CYP1A2 genetic polymorphism and various related constitutional hereditary factors.

著者

Toshiki UEDA Teruaki TOZAKI Satoshi NOZAWA Kenji KINOSHITA Hitoshi GAWAHARA

出版者

Japanese Society of Equine Science

雑誌

Journal of Equine Science (ISSN:13403516)

巻号頁・発行日

vol.30, no.3, pp.55-61, 2019 (Released:2019-10-02)

参考文献数

23

被引用文献数

7

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Recently, the illegal use of novel technologies, such as gene and cell therapies, has become a great concern for the horseracing industry. As a potential way to control this, metabolomics approaches that comprehensively analyze metabolites in biological samples have been gaining attention. However, it may be difficult to identify metabolic biomarkers for doping because physiological conditions generally differ between resting and exercise states in horses. To understand the metabolic differences in horse plasma between the resting state at training centres and the sample collection stage after racing for doping test (SAD), we took plasma samples from these two stages (n=30 for each stage) and compared the metabolites present in these samples by liquid chromatography-high resolution mass spectrometry. This analysis identified 5,010 peaks, of which 1,256 peaks (approximately 25%) were annotated using KEGG analysis. Principal component analysis showed that the resting state and SAD groups had entirely different metabolite compositions. In particular, the levels of inosine, xanthosine, uric acid, and allantoin, which are induced by extensive exercise, were significantly increased in the SAD group. In addition, many metabolites not affected by extensive exercise were also identified. These results will contribute to the discovery of biomarkers for detecting doping substances that cannot be detected by conventional methods.