著者

Kosono S Kataoka M

出版者

The Society for Actinomycetes Japan

雑誌

Actinomycetologica (ISSN:09145818)

巻号頁・発行日

vol.10, no.1, pp.12-22, 1996

被引用文献数

1

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pSN22, an 11 kb multicopy conjugative plasmid from Streptomyces nigrifaciens, promotes chromosome recombination in Streptomyces lividans. Five genes have been identified to be involved in plasmid transfer and pock formation: traB is essential for plasmid transfer; traA for pock formation; spdA and spdB are concerned with pock size; and traR, which corresponds to a kor gene in a kil-kor system, encodes a repressor of traR itself and the traA-traB-spdB (tra) operon. Studies on the interaction of TraR with promoter regions suggest that the negative regulation of transfer-related genes by TraR is achieved by two mechanisms, i.e. promoter hiding and roadblock. The predicted ATPase activity and the membrane localization of TraB suggest that the protein plays a direct role in ATP-driven DNA translocation. TraB is also thought to be involved in intra- and intermycelial transfers of pSN22.