- 著者
- Eyal Kurzbaum Yoram Zimmels Robert Armon
- 出版者
- The Society for Actinomycetes Japan
- 雑誌
- 日本放線菌学会誌 (ISSN:09145818)
- 巻号頁・発行日
- vol.24, no.2, pp.31-38, 2010-12-25 (Released:2010-12-15)
- 参考文献数
- 52
- 被引用文献数
- 4 9
In the present study, Streptomyces sp. CW1 was isolated from a constructed wetland system mesocosm and identified as such based on 16S rDNA analysis, and additional biochemical properties were evaluated. This isolate was found to be halotolerant (up to 11% NaCl) and able to grow and utilize biopolymers such as: agarose, agar, gellan gum (a bacterial polysaccharide), polypectate and chitin as the sole carbon and energy source. Since the primary isolation was performed based on its capability to degrade phenol, the high cell yield coefficient showed rapid growth on phenol (0.82–0.98 mg dry biomass/mg phenol). Its halotolerance and the capability to biodegrade biopolymers found mainly in the marine environments, suggest on its primordial oceanic origin. In the present study some of its characteristics are described and discussed along with its beneficial use for wastewater purification processes in constructed wetland systems.
- 著者
- Kosono S Kataoka M
- 出版者
- The Society for Actinomycetes Japan
- 雑誌
- Actinomycetologica (ISSN:09145818)
- 巻号頁・発行日
- vol.10, no.1, pp.12-22, 1996
- 被引用文献数
- 1
pSN22, an 11 kb multicopy conjugative plasmid from Streptomyces nigrifaciens, promotes chromosome recombination in Streptomyces lividans. Five genes have been identified to be involved in plasmid transfer and pock formation: traB is essential for plasmid transfer; traA for pock formation; spdA and spdB are concerned with pock size; and traR, which corresponds to a kor gene in a kil-kor system, encodes a repressor of traR itself and the traA-traB-spdB (tra) operon. Studies on the interaction of TraR with promoter regions suggest that the negative regulation of transfer-related genes by TraR is achieved by two mechanisms, i.e. promoter hiding and roadblock. The predicted ATPase activity and the membrane localization of TraB suggest that the protein plays a direct role in ATP-driven DNA translocation. TraB is also thought to be involved in intra- and intermycelial transfers of pSN22.