- 著者
- Mami OBA Tsutomu OMATSU Ai TAKANO Hiromi FUJITA Kozue SATO Atsushi NAKAMOTO Mamoru TAKAHASHI Nobuhiro TAKADA Hiroki KAWABATA Shuji ANDO Tetsuya MIZUTANI
- 出版者
- 公益社団法人 日本獣医学会
- 雑誌
- Journal of Veterinary Medical Science (ISSN:09167250)
- 巻号頁・発行日
- pp.15-0536, (Released:2015-10-25)
- 被引用文献数
- 1 25
Soft ticks, Argas vespertilionis, were collected from feces of bats in Japan. Cytopathic effect (CPE) was observed after inoculating the homogenates of ticks to Vero cells. Sequencing of RNA extracted from the cell supernatant was performed by next generation sequencer. The contigs had identity to segments of Bunyaviruses, Issyk-Kul virus. The identities of segment L, M and S were only 77, 76 and 79% to Issyk-Kul virus, respectively. Therefore, we named this novel virus Soft tick bunyavirus (STBV). In the phylogenetic tree, segment L of STBV was closely related to a cluster consisting of the genus Nairovirus of the family Bunyaviridae.
- 著者
- Hiroki KAWABATA Ai TAKANO Teruki KADOSAKA Hiromi FUJITA Yoshiki NITTA Mutsuyo GOKUDEN Toshiro HONDA Junko TOMIDA Yoshiaki KAWAMURA Toshiyuki MASUZAWA Fubito ISHIGURO Nobuhiro TAKADA Yasuhiro YANO Masako ANDOH Shuji ANDO Kozue SATO Hideyuki TAKAHASHI Makoto OHNISHI
- 出版者
- JAPANESE SOCIETY OF VETERINARY SCIENCE
- 雑誌
- Journal of Veterinary Medical Science (ISSN:09167250)
- 巻号頁・発行日
- pp.13-0162, (Released:2013-05-13)
- 被引用文献数
- 4 7
Lyme disease Borrelia spp. are transmitted by Ixodes ticks, and more than 10 species of borreliae have been identified around the world. Recently another Borrelia sp. has been reported in Asia (Japan, Korea, China, Taiwan and Thailand) as Borrelia valaisiana-related sp. In the present study, we obtained and genetically characterized 19 B. valaisiana-related sp. strains from mammals and ticks. Genetic analyses showed that the Borrelia strains were distinct from B. valaisiana found in Europe. Multilocus sequencing typing revealed that these Borrelia isolates formed a monophyletic group with B. yangtze strains in China. Some of strains were isolated from the bladders of small mammals, and also two strains were experimentally confirmed to be infectious to C3H/HeN mice. We observed that the Borrelia sp. was maintained in Ixodes granulatus tick after molting. These results suggested that small mammals and I. granulatus were possible reservoir hosts and vector tick for the Borrelia sp., respectively. B. valaisiana, originally found in Europe, was transmitted mainly by I. ricinus, and birds were mainly thought to be reservoir hosts. Our results suggested Japanese isolates of B. yangtze (formerly B. valaisiana-related sp.) were distinguishable from B. valaisiana according to the reservoir host and its vector tick. In this study, we also deposited borrelia strain Okinawa-CW62 to bioresource centers as a reference strain of the B. yangtze (=DSM 24625, JCM 17189).
- 著者
- Hiroki KAWABATA Ai TAKANO Teruki KADOSAKA Hiromi FUJITA Yoshiki NITTA Mutsuyo GOKUDEN Toshiro HONDA Junko TOMIDA Yoshiaki KAWAMURA Toshiyuki MASUZAWA Fubito ISHIGURO Nobuhiro TAKADA Yasuhiro YANO Masako ANDOH Shuji ANDO Kozue SATO Hideyuki TAKAHASHI Makoto OHNISHI
- 出版者
- 公益社団法人 日本獣医学会
- 雑誌
- Journal of Veterinary Medical Science (ISSN:09167250)
- 巻号頁・発行日
- vol.75, no.9, pp.1201-1207, 2013 (Released:2013-10-01)
- 参考文献数
- 47
- 被引用文献数
- 2 7
Lyme disease Borrelia spp. are transmitted by Ixodes ticks, and more than 10 species of borreliae have been identified around the world. Recently, another Borrelia sp. has been reported in Asia (Japan, Korea, China, Taiwan and Thailand) as Borrelia valaisiana-related sp. In the present study, we obtained and genetically characterized 19 B. valaisiana-related sp. strains from mammals and ticks. Genetic analyses showed that the Borrelia strains were distinct from B. valaisiana found in Europe. Multilocus sequence typing revealed that these Borrelia isolates formed a monophyletic group with B. yangtze strains in China. Some of the strains were isolated from the bladders of small mammals, and also two strains were experimentally confirmed to be infectious in C3H/HeN mice. We observed that the Borrelia sp. was maintained in the Ixodes granulatus tick after molting. These results suggested that small mammals and I. granulatus were possible reservoir hosts and the vector tick for the Borrelia sp., respectively. B. valaisiana, originally found in Europe, was transmitted mainly by I. ricinus, and birds were mainly thought to be reservoir hosts. Our results suggested that Japanese isolates of B. yangtze (formerly B. valaisiana-related sp.) were distinguishable from B. valaisiana according to the reservoir host and its vector tick. In this study, we also deposited borrelia strain Okinawa-CW62 into bioresource centers as a reference strain of B. yangtze(=DSM 24625, JCM 17189).
- 著者
- Hisashi INOKUMA Shigeki MAETANI Jyunji FUJITSUKA Ai TAKANO Kozue SATO Takashi FUKUI Toshiyuki MASUZAWA Hiroki KAWABATA
- 出版者
- 公益社団法人 日本獣医学会
- 雑誌
- Journal of Veterinary Medical Science (ISSN:09167250)
- 巻号頁・発行日
- vol.75, no.7, pp.975-978, 2013 (Released:2013-07-31)
- 参考文献数
- 26
- 被引用文献数
- 2 8
Two dogs that exhibited sudden astasia, anorexia and fever higher than 40°C were suspected of having Lyme disease in July 2011. Clinical symptoms gradually improved with antibiotic treatment in both cases. Polymerase chain reaction and sequence analysis revealed Borrelia garinii DNA fragments in the peripheral blood in the acute disease phase. Serological tests, including enzyme linked immunosorbent assay and Western blot analysis, showed an increased IgG antibody titer against Borrelia pathogens in one of the dogs. These findings suggested that diagnosis of the two dogs was Lyme disease related to B. garinii infection.
- 著者
- Hisashi INOKUMA Shigeki MAETANI Jyunji FUJITSUKA Ai TAKANO Kozue SATO Takashi FUKUI Toshiyuki MASUZAWA Hiroki KAWABATA
- 出版者
- 公益社団法人 日本獣医学会
- 雑誌
- Journal of Veterinary Medical Science (ISSN:09167250)
- 巻号頁・発行日
- pp.13-0027, (Released:2013-02-22)
- 被引用文献数
- 2 8
Two dogs that exhibited sudden astasia, anorexia and fever higher than 40°C were suspected of having Lyme disease in July 2011. Clinical symptoms gradually improved with antibiotic treatment in both cases. Polymerase chain reaction and sequence analysis revealed Borrelia garinii DNA fragments in the peripheral blood in the acute disease phase. Serological tests, including enzyme linked immunosorbent assay and Western blot analysis, showed an increased IgG antibody titer against Borrelia pathogens in one of the dogs. These findings suggested that diagnosis of the two dogs were Lyme disease related to B. garinii infection.