- 著者
- Hiroki KAWABATA Ai TAKANO Teruki KADOSAKA Hiromi FUJITA Yoshiki NITTA Mutsuyo GOKUDEN Toshiro HONDA Junko TOMIDA Yoshiaki KAWAMURA Toshiyuki MASUZAWA Fubito ISHIGURO Nobuhiro TAKADA Yasuhiro YANO Masako ANDOH Shuji ANDO Kozue SATO Hideyuki TAKAHASHI Makoto OHNISHI
- 出版者
- JAPANESE SOCIETY OF VETERINARY SCIENCE
- 雑誌
- Journal of Veterinary Medical Science (ISSN:09167250)
- 巻号頁・発行日
- pp.13-0162, (Released:2013-05-13)
- 被引用文献数
- 4 7
Lyme disease Borrelia spp. are transmitted by Ixodes ticks, and more than 10 species of borreliae have been identified around the world. Recently another Borrelia sp. has been reported in Asia (Japan, Korea, China, Taiwan and Thailand) as Borrelia valaisiana-related sp. In the present study, we obtained and genetically characterized 19 B. valaisiana-related sp. strains from mammals and ticks. Genetic analyses showed that the Borrelia strains were distinct from B. valaisiana found in Europe. Multilocus sequencing typing revealed that these Borrelia isolates formed a monophyletic group with B. yangtze strains in China. Some of strains were isolated from the bladders of small mammals, and also two strains were experimentally confirmed to be infectious to C3H/HeN mice. We observed that the Borrelia sp. was maintained in Ixodes granulatus tick after molting. These results suggested that small mammals and I. granulatus were possible reservoir hosts and vector tick for the Borrelia sp., respectively. B. valaisiana, originally found in Europe, was transmitted mainly by I. ricinus, and birds were mainly thought to be reservoir hosts. Our results suggested Japanese isolates of B. yangtze (formerly B. valaisiana-related sp.) were distinguishable from B. valaisiana according to the reservoir host and its vector tick. In this study, we also deposited borrelia strain Okinawa-CW62 to bioresource centers as a reference strain of the B. yangtze (=DSM 24625, JCM 17189).
- 著者
- Toshiyuki Masuzawa Shou Masuda Takashi Fukui Yoshihiro Okamoto Jantsandoo Bataa Yosaburo Oikawa Fubito Ishiguro Nobuhiro Takada
- 出版者
- 国立感染症研究所 Japanese Journal of Infectious Diseases 編集委員会
- 雑誌
- Japanese Journal of Infectious Diseases (ISSN:13446304)
- 巻号頁・発行日
- vol.67, no.1, pp.47-49, 2014 (Released:2014-01-22)
- 参考文献数
- 26
- 被引用文献数
- 2 19
A molecular epidemiological survey was conducted to identify the tick-borne disease agents Anaplasma phagocytophilum and Borrelia burgdorferi sensu lato in Selenge Province, Mongolia. The survey was in response to a suspected A. phagocytophilum infection in a patient. In 2012, a total of 129 questing Ixodes persulcatus adult ticks were sampled by flagging vegetation. A. phagocytophilum and Borrelia spp. were detected by PCR, targeting the 16S rDNA (rrs) and 5S–23S intergenic spacer region, respectively. Infection rates for A. phagocytophilum and B. burgdorferi sensu lato spp. were 6.2% and 55.0%, respectively. Six of the 129 ticks (4.9%) were coinfected with A. phagocytophilum and B. burgdorferi sensu lato. Among Borrelia spp., the highest prevalence rate was that for B. garinii 20047 type (26.3%), followed by B. afzelii (7.8%) and B. garinii NT29 type (7.0%). Furthermore, ticks were detected that were dually infected with B. afzelii and B. garinii 20047 type (7.8%) and B. garinii NT29 and 20047 types (6.2%).
- 著者
- Hiroki KAWABATA Ai TAKANO Teruki KADOSAKA Hiromi FUJITA Yoshiki NITTA Mutsuyo GOKUDEN Toshiro HONDA Junko TOMIDA Yoshiaki KAWAMURA Toshiyuki MASUZAWA Fubito ISHIGURO Nobuhiro TAKADA Yasuhiro YANO Masako ANDOH Shuji ANDO Kozue SATO Hideyuki TAKAHASHI Makoto OHNISHI
- 出版者
- 公益社団法人 日本獣医学会
- 雑誌
- Journal of Veterinary Medical Science (ISSN:09167250)
- 巻号頁・発行日
- vol.75, no.9, pp.1201-1207, 2013 (Released:2013-10-01)
- 参考文献数
- 47
- 被引用文献数
- 2 7
Lyme disease Borrelia spp. are transmitted by Ixodes ticks, and more than 10 species of borreliae have been identified around the world. Recently, another Borrelia sp. has been reported in Asia (Japan, Korea, China, Taiwan and Thailand) as Borrelia valaisiana-related sp. In the present study, we obtained and genetically characterized 19 B. valaisiana-related sp. strains from mammals and ticks. Genetic analyses showed that the Borrelia strains were distinct from B. valaisiana found in Europe. Multilocus sequence typing revealed that these Borrelia isolates formed a monophyletic group with B. yangtze strains in China. Some of the strains were isolated from the bladders of small mammals, and also two strains were experimentally confirmed to be infectious in C3H/HeN mice. We observed that the Borrelia sp. was maintained in the Ixodes granulatus tick after molting. These results suggested that small mammals and I. granulatus were possible reservoir hosts and the vector tick for the Borrelia sp., respectively. B. valaisiana, originally found in Europe, was transmitted mainly by I. ricinus, and birds were mainly thought to be reservoir hosts. Our results suggested that Japanese isolates of B. yangtze (formerly B. valaisiana-related sp.) were distinguishable from B. valaisiana according to the reservoir host and its vector tick. In this study, we also deposited borrelia strain Okinawa-CW62 into bioresource centers as a reference strain of B. yangtze(=DSM 24625, JCM 17189).