- 著者
- Narusaka Mari Kubo Yasuyuki Hatakeyama Katsunori Imamura Jun Ezura Hiroshi Nanasato Yoshihiko Tabei Yutaka Takano Yoshitaka
- 出版者
- Public Library of Science
- 雑誌
- PLoS ONE (ISSN:19326203)
- 巻号頁・発行日
- vol.8, no.2, pp.e55954, 2013-02
- 被引用文献数
- 77 10
A major class of disease resistance (R) genes which encode nucleotide binding and leucine rich repeat (NB-LRR) proteins have been used in traditional breeding programs for crop protection. However, it has been difficult to functionally transfer NB-LRR-type R genes in taxonomically distinct families. Here we demonstrate that a pair of Arabidopsis (Brassicaceae) NB-LRR-type R genes, RPS4 and RRS1, properly function in two other Brassicaceae, Brassica rapa and Brassica napus, but also in two Solanaceae, Nicotiana benthamiana and tomato (Solanum lycopersicum). The solanaceous plants transformed with RPS4/RRS1 confer bacterial effector-specific immunity responses. Furthermore, RPS4 and RRS1, which confer resistance to a fungal pathogen Colletotrichum higginsianum in Brassicaceae, also protect against Colletotrichum orbiculare in cucumber (Cucurbitaceae). Importantly, RPS4/RRS1 transgenic plants show no autoimmune phenotypes, indicating that the NB-LRR proteins are tightly regulated. The successful transfer of two R genes at the family level implies that the downstream components of R genes are highly conserved. The functional interfamily transfer of R genes can be a powerful strategy for providing resistance to a broad range of pathogens.
- 著者
- Fujii Isao Mori Yuichiro Watanabe Akira KUBO Yasuyuki TSUJI Gento EBIZUKA Yutaka
- 出版者
- 社団法人日本農芸化学会
- 雑誌
- Bioscience, biotechnology, and biochemistry (ISSN:09168451)
- 巻号頁・発行日
- vol.63, no.8, pp.1445-1452, 1999-08-23
- 参考文献数
- 33
- 被引用文献数
- 9 80
The Colletotrichum lagenarium PKS1 gene was expressed in the heterologous fungal host, Aspergillus oryzae, under the starch-inducible α-amylase promoter to identify the direct product of polyketide synthase (PKS) encoded by the PKS1 gene. The main compound produced by an A.oryzae transformant was isolated and characterized to be 1,3,6,8-tetrahydroxynaphthalene (T4HN) as its tetraacetate. Since the PKS1 gene was cloned from C.lagenarium to complement the nonmelanizing albinio mutant, T4HN was assumed to be an initial biosynthetic intermediate, and thus the product of the PKS reaction, but had not been isolated from the fungus. The production of T4HN by the PKS1 transformant unambiguously identified the gene to encode a PKS of pentaketide T4HN. In addition, tetraketide orsellinic acid and pentaketide isocoumarin were isolated, the latter being derived from a pentaketide monocyclic carboxylic acid, as by-products of the PKS1 PKS reaction. Production of the pentaketide carboxylic acid provided insights into the mechanism for the PKS1 polyketide synthase reaction to form T4HN.