著者

LIU Xiaobo EICHENBERGER Michael FUJIOKA Yuuichiro DONG Jinhua UEDA Hiroshi

出版者

The Japan Society for Analytical Chemistry

雑誌

Analytical Sciences (ISSN:09106340)

巻号頁・発行日

vol.28, no.9, pp.861-867, 2012

被引用文献数

10

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The development of a rapid and specific assay for 17β-estradiol (E2) will accelerate its <i>in vitro</i> diagnostics and/or environmental pollution control. Here, we employed an open-sandwich (OS) selection scheme to improve the sensitivity for E2 in an OS immunoassay, which is based on antigen-dependent stabilization of the antibody (Ab) variable region, Fv, where the two domains (V_H and V_L) dissociated in the absence of an antigen. The V_H domain of a cloned anti-E2 antibody displayed on M13 phage was randomly mutated, and after three OS biopanning rounds, a mutant that showed higher sensitivity in OS-ELISA for E2 was identified. Interestingly, compared with the wild-type V_H, the cross-reactivity of the mutant was significantly decreased for the analogous steroid testosterone, both in OS and competitive ELISAs. This is the first report concerning selection for an anti-hapten Ab without using any hapten-carrier conjugates, and the method will be especially suitable for selecting Ab fragments that show better performance in hapten OS immunoassays.

著者

Liu Xiaobo Lin Yuhan Chen Yanming An Lijia Ji Xiangling Jiang Bingzheng

出版者

The Chemical Society of Japan

雑誌

Chemistry Letters (ISSN:03667022)

巻号頁・発行日

vol.34, no.9, pp.1284-1285, 2005

被引用文献数

6

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High-quality CdSe quantum dots have been synthesized through an organometallic method with cadmium methoxide as a cadmium precursor. Without any size sorting, the FWHM (full width at half-maximum) in PL spectra almost for all CdSe samples is less than 30 nm and only 22 nm from the best sample. The emission peaks with different particle size in toluene can be tuned from 478 to 654 nm corresponding to a color change from blue-green to red.