- 著者
- Ohtsuka Susumu El-Sherbiny Mohsen M. Ueda Hiroshi
- 出版者
- 日本甲殻類学会
- 雑誌
- Crustacean research (ISSN:02873478)
- 巻号頁・発行日
- no.29, pp.1-11, 2000-12
- 被引用文献数
- 3
Two new species of Tortanus (Atortus), T. (A.) ampliramus and T. (A.) nishidai, are described from the northern Red Sea and the Gulf of Aden, respectively. These new species evidently belong to the recticauda species group in the recticauda species complex. The species group has a distinct distribution in the western Indian Ocean, extending from South Africa. The functional morphology of spermatophores of Tortanus is also discussed. A semelparous reproductive strategy may be employed in some species of Tortanus, whereas others adopt an iteroparous strategy as do most of centropagoideans. The formation of swarms was found exclusively in the subgenus Atortus which inhabits clear waters. Swarms of T. (A.) erabuensis may utilize swarms of another small copepod, Oithona oculata as prey.
- 著者
- LIU Xiaobo EICHENBERGER Michael FUJIOKA Yuuichiro DONG Jinhua UEDA Hiroshi
- 出版者
- The Japan Society for Analytical Chemistry
- 雑誌
- Analytical Sciences (ISSN:09106340)
- 巻号頁・発行日
- vol.28, no.9, pp.861-867, 2012
- 被引用文献数
- 10
The development of a rapid and specific assay for 17β-estradiol (E2) will accelerate its <i>in vitro</i> diagnostics and/or environmental pollution control. Here, we employed an open-sandwich (OS) selection scheme to improve the sensitivity for E2 in an OS immunoassay, which is based on antigen-dependent stabilization of the antibody (Ab) variable region, Fv, where the two domains (V<sub>H</sub> and V<sub>L</sub>) dissociated in the absence of an antigen. The V<sub>H</sub> domain of a cloned anti-E2 antibody displayed on M13 phage was randomly mutated, and after three OS biopanning rounds, a mutant that showed higher sensitivity in OS-ELISA for E2 was identified. Interestingly, compared with the wild-type V<sub>H</sub>, the cross-reactivity of the mutant was significantly decreased for the analogous steroid testosterone, both in OS and competitive ELISAs. This is the first report concerning selection for an anti-hapten Ab without using any hapten-carrier conjugates, and the method will be especially suitable for selecting Ab fragments that show better performance in hapten OS immunoassays.
1 0 0 0 OA 聖ベルナールの「雅歌についての説教」とその古フランス語訳について
- 著者
- 植田 裕志 Ueda Hiroshi
- 出版者
- 名古屋大学文学部
- 雑誌
- 名古屋大學文學部研究論集 (ISSN:04694716)
- 巻号頁・発行日
- vol.43, pp.87-102, 1997-03-31 (Released:2006-02-14)
1 0 0 0 OA 中世ロマンと地理 : 「イポメドン」をめぐって
- 著者
- 植田 裕志 Ueda Hiroshi
- 出版者
- 名古屋大学文学部
- 雑誌
- 名古屋大學文學部研究論集 (ISSN:04694716)
- 巻号頁・発行日
- vol.41, pp.131-146, 1995-03-31 (Released:2006-02-14)