- 著者
- ATSUO IWASAWA YOSHIMI NIWANO TAKAYUKI MOKUDAI MASAHIRO KOHNO
- 出版者
- The Society for Antibacterial and Antifungal Agents, Japan
- 雑誌
- Biocontrol Science (ISSN:13424815)
- 巻号頁・発行日
- vol.14, no.3, pp.107-111, 2009 (Released:2010-01-26)
- 参考文献数
- 29
- 被引用文献数
- 18 29
Proanthocyanidin, which consists of (+) catechin, (-) epicatechin and their gallates (15%), (-) epicatechin gallate-dimers, -trimers, and -tetramers (80%), and (-) epicatechin gallate-pentamers, -hexamers, and -heptamers (5%), was evaluated for its antiviral activity against feline calicivirus F9 strain (FCV/F9), which is thought to be a surrogate for noroviruses, and coxsackievirus A7 strain (Cox.A7), which was selected as a representative enteric virus. To achieve a viral inactivation rate of 99% or greater after contact for 10 sec., at least 1 mg/ml and 10 mg/ml of proanthocyanidin were required against FCV/F9 and Cox.A7, respectively. Although the antiviral mechanism of proanthocyanidin is not clear at present, proanthocyanidin may be an effective disinfectant against enteroviruses such as noroviruses.
- 著者
- Keishi MATSUO Yoshiyuki TAKATSUJI Masahiro KOHNO Toshiaki KAMACHI Hideo NAKADA Tetsuya HARUYAMA
- 出版者
- The Electrochemical Society of Japan
- 雑誌
- Electrochemistry (ISSN:13443542)
- 巻号頁・発行日
- vol.83, no.9, pp.721-724, 2015-09-05 (Released:2015-09-05)
- 参考文献数
- 10
- 被引用文献数
- 7 12
Efficient generation of oxygen radicals and reactive oxygen was successfully performed at the dispersed-phasic interface between vapor-water and oxygen plasma in a reaction chamber having an internal atmosphere with a normal-pressure and temperature. In the space of the reactor chamber (radical vapor reactor [RVR]), the gas phase was strictly controlled in terms of vaporized water (small water mist), temperature, plasma conditions, and UV irradiation. According to spin-trapping electron spin resonance analysis, the RVR efficiently and quantitatively yielded two types of reactive oxygen species (1O2 and OH radical) with the atmosphere of the RVR chamber. This is the report of the efficient, quantitative production of reactive oxygen in an atmosphere. The reactivity of the produced 1O2 and OH radical may be applicable for various chemical processes, such as oxidation and electron absorption.
- 著者
- Masahiro Kohno Motohiro Takeda Yoshimi Niwano Rumiko Saito Natsumi Emoto Mika Tada Takemichi Kanazawa Noriaki Ohuchi Rie Yamada
- 出版者
- 東北ジャーナル刊行会
- 雑誌
- The Tohoku Journal of Experimental Medicine (ISSN:00408727)
- 巻号頁・発行日
- vol.216, no.1, pp.47-52, 2008 (Released:2008-08-22)
- 参考文献数
- 19
- 被引用文献数
- 3 8
Early detection and optimal treatment are the most effective means to improve cancer mortality. Mass screening for cancer has yielded a marked reduction of cancer mortality in the United States. Simple and effective methods are expected for screening of malignancy. Hematoporphyrin derivatives (HPDs) are known to accumulate in cancer cells; thus, HPD has been used for local diagnosis and photodynamic therapy of cancer. The lymphocytes of cancer patients also demonstrate the active uptake of HPD and this phenomenon has been applied for the diagnosis of cancer. In the present study, we have developed a novel method for measurement of the chemiluminescence of HPD in peripheral blood lymphocytes. HPD is composed of hematoporphyrin and its oligomers. Seven cancer patients and seven controls were recruited for this study. The primary cancers included two prostate cancers (one without metastasis and the other with lung metastasis), a renal cancer, a lung adenocarcinoma with systemic metastasis, two gallbladder cancers with lung metastasis, and a colon cancer with liver metastasis. HPD in lymphocytes was measured using a highly sensitive chemiluminescence analyzer with laser light irradiation to detect photoemission by 1O2 from HPD. The intensity of chemiluminescence exhibited a linear correlation with the concentrations of HPD. In addition, the level of HPD in lymphocytes was significantly higher in cancer patients than that in healthy volunteers (p < 0.05). These results suggest that detection of the chemiluminescence of HPD in lymphocytes could be a sensitive and simple method for cancer diagnosis and screening.
- 著者
- Keisuke Nakayama Masaaki Nakayama Hiroyuki Terawaki Yaeko Murata Toshinobu Sato Masahiro Kohno Sadayoshi Ito
- 出版者
- 日本毒性学会
- 雑誌
- The Journal of Toxicological Sciences (ISSN:03881350)
- 巻号頁・発行日
- vol.34, no.6, pp.699-702, 2009-12-01 (Released:2009-12-01)
- 参考文献数
- 13
- 被引用文献数
- 5 9
Carbonated soft drinks reportedly contain methylglyoxal (MG), which is strongly associated with human carbonyl stress. We sought to evaluate the effects of carbonated drink intake on human carbonyl stress. We measured MG levels in 4 commercial beverage brands, and evaluated the changes in plasma MG in healthy subjects following the intake of carbonated drinks. By 30 min after intake of samples containing high glucose and high MG, the levels of plasma MG, glucose, insulin and uric acid had increased significantly, and then returned to basal levels by 120 min. After intake of the low-calorie carbonated samples containing little MG, there were no increases in plasma MG. Our results suggest that glucose-containing carbonated soft drinks are associated with increases in not only glucose but also carbonyl burden.