著者

Makino Yukiko Tanaka Satoshi Kurobane Shingo NAKAUCHI Masato TERASAKI Takahiro OHTA Shigeru

出版者

公益社団法人日本薬学会

雑誌

Journal of health science (ISSN:13449702)

巻号頁・発行日

vol.49, no.2, pp.129-137, 2003-04-01

参考文献数

7

被引用文献数

4 23

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We have investigated 100 illegal amphetamine-type stimulant (ATS) tablets seized in Japan to obtain information about the nature of these tablets abused in Japan. For physical characterization, 15 items (logo, vertical view, colour, diameter, weight, smell, <I>etc</I>.) were measured and a photograph was taken. For chemical characterization, the components in the tablet were identified by GC-MS and HPLC, and quantified by HPLC using an ODS-type column. The maximum content of <I>N</I>-methyl-3,4-methylenedioxyamphetamine (MDMA) was 189 mg/tablet and that of 3,4-methylenedioxyamphetamine (MDA) was 87 mg/tablet. The detected components, other than MDMA and MDA, were <I>N</I>-ethyl-3,4-methylenedioxyamphetamine (MDEA), ephedrine, caffeine, ketamine, and methamphetamine. In this report, we propose a method for profiling ATS tablets.

著者

Yamaguchi Mitsune Saito Toshiaki Horiguchi Yoshie Ogawa Keita Tsuchiya Yukihiro Hishinuma Kayoko Chikuma Toshiyuki Makino Yukiko Hojo Hiroshi

出版者

公益社団法人日本薬学会

雑誌

Journal of health science (ISSN:13449702)

巻号頁・発行日

vol.50, no.6, pp.600-604, 2004-12-01

被引用文献数

4

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The cultivation or trafficking of "Magic mushrooms," containing hallucinogenic psilocin and psilocybin, has been prohibited by the Narcotics and Psychotropics Control Law in Japan since 2002. To identify these mushrooms, we attempted to prepare the monoclonal antibody (mAb) reactive to these hallucinogens. As an antigen inducing an anti-psilocin mAb, N-{4-[3-(2-dimethylaminoethyl)indol-4-yl-oxy]butyl}succinamic acid was synthesized by modifying the 4-hydroxyl moiety of psilocin and coupled to a carrier protein of keyhole limpet hemocyanin. BALB/c mice were immunized five times with the antigen emulsified with an adjuvant, and their spleen cells were fused with mouse myeloma cells. We obtained several hybridoma cells producing mAbs reactive to psilocin, from which fourclones, BA631, CA231, KA422, and MA332 with a higher production of anti-psilocin mAb were selected by limiting dilution. Isotype of CA231 and KA422 mAbs were IgG2a and that of BA631 and MA332 mAbs were IgGl.Enzyme immunoassay (EIA) using BA631 mAb, revealed that BA631 cross-reacted with psilocin and dimethyltryptamine, but not with the other indole derivatives such as psilocybin, 4-hydroxyindole, tryptamine, and tryptophan. Therefore, these antibodies could be used for the identification of magic mushrooms.