- 著者
-
Imanishi Tadashi
Itoh Takeshi
Suzuki Yutaka
O'Donovan Claire
Fukuchi Satoshi
Koyanagi Kanako O.
Barrero Roberto A.
Tamura Takuro
Yamaguchi-Kabata Yumi
Tanino Motohiko
Yura Kei
Miyazaki Satoru
Ikeo Kazuho
Homma Keiichi
Kasprzyk Arek
Nishikawa Tetsuo
Hirakawa Mika
Thierry-Mieg Jean
Thierry-Mieg Danielle
Ashurst Jennifer
Jia Libin
Nakao Mitsuteru
Thomas Michael A.
Mulder Nicola
Karavidopoulou Youla
Jin Lihua
Kim Sangsoo
Yasuda Tomohiro
Lenhard Boris
Eveno Eric
Suzuki Yoshiyuki
Yamasaki Chisato
Takeda Jun-ichi
Gough Craig
Hilton Phillip
Fujii Yasuyuki
Sakai Hiroaki
Tanaka Susumu
Amid Clara
Bellgard Matthew
Bonaldo Maria de Fatima
Bono Hidemasa
Bromberg Susan K.
Brookes Anthony J.
Bruford Elspeth
Carninci Piero
Chelala Claude
Couillault Christine
Souza Sandro J. de
Debily Marie-Anne
Devignes Marie-Dominique
Dubchak Inna
Endo Toshinori
Estreicher Anne
Eyras Eduardo
Fukami-Kobayashi Kaoru
R. Gopinath Gopal
Graudens Esther
Hahn Yoonsoo
Han Michael
Han Ze-Guang
Hanada Kousuke
Hanaoka Hideki
Harada Erimi
Hashimoto Katsuyuki
Hinz Ursula
Hirai Momoki
Hishiki Teruyoshi
Hopkinson Ian
Imbeaud Sandrine
Inoko Hidetoshi
Kanapin Alexander
Kaneko Yayoi
Kasukawa Takeya
Kelso Janet
Kersey Paul
Kikuno Reiko
Kimura Kouichi
Korn Bernhard
Kuryshev Vladimir
Makalowska Izabela
Makino Takashi
Mano Shuhei
Mariage-Samson Regine
Mashima Jun
Matsuda Hideo
Mewes Hans-Werner
Minoshima Shinsei
Nagai Keiichi
Nagasaki Hideki
Nagata Naoki
Nigam Rajni
Ogasawara Osamu
Ohara Osamu
Ohtsubo Masafumi
Okada Norihiro
Okido Toshihisa
Oota Satoshi
Ota Motonori
Ota Toshio
Otsuki Tetsuji
Piatier-Tonneau Dominique
Poustka Annemarie
Ren Shuang-Xi
Saitou Naruya
Sakai Katsunaga
Sakamoto Shigetaka
Sakate Ryuichi
Schupp Ingo
Servant Florence
Sherry Stephen
Shiba Rie
Shimizu Nobuyoshi
Shimoyama Mary
Simpson Andrew J.
Soares Bento
Steward Charles
Suwa Makiko
Suzuki Mami
Takahashi Aiko
Tamiya Gen
Tanaka Hiroshi
Taylor Todd
Terwilliger Joseph D.
Unneberg Per
Veeramachaneni Vamsi
Watanabe Shinya
Wilming Laurens
Yasuda Norikazu
Yoo Hyang-Sook
Stodolsky Marvin
Makalowski Wojciech
Go Mitiko
Nakai Kenta
Takagi Toshihisa
Kanehisa Minoru
Sakaki Yoshiyuki
Quackenbush John
Okazaki Yasushi
Hayashizaki Yoshihide
Hide Winston
Chakraborty Ranajit
Nishikawa Ken
Sugawara Hideaki
Tateno Yoshio
Chen Zhu
Oishi Michio
Tonellato Peter
Apweiler Rolf
Okubo Kousaku
Wagner Lukas
Wiemann Stefan
Strausberg Robert L.
Isogai Takao
Auffray Charles
Nomura Nobuo
Gojobori Takashi
Sugano Sumio
- 出版者
- Public Library of Science
- 雑誌
- PLoS Biology (ISSN:15449173)
- 巻号頁・発行日
- vol.2, no.6, pp.856-875, 2004-06
- 被引用文献数
-
3
226
The human genome sequence defines our inherent biological potential; the realization of the biology encoded therein requires knowledge of the function of each gene. Currently, our knowledge in this area is still limited. Several lines of investigation have been used to elucidate the structure and function of the genes in the human genome. Even so, geneprediction remains a difficult task, as the varieties of transcripts of a gene may vary to a great extent. We thus performed an exhaustive integrative characterization of 41,118 full-length cDNAs that capture the gene transcripts as complete functional cassettes, providing an unequivocal report of structural and functional diversity at the gene level.Our international collaboration has validated 21,037 human gene candidates by analysis of high-quality full-length cDNA clones through curation using unified criteria. This led to the identification of 5,155 new gene candidates. It also manifested the most reliable way to control the quality of the cDNA clones. We have developed a human gene database, called the H-Invitational Database (H-InvDB; http://www.h-invitational.jp/). It provides the following:integrative annotation of human genes, description of gene structures, details of novel alternative splicing isoforms, non-protein-coding RNAs, functional domains, subcellular localizations, metabolic pathways, predictions of protein three-dimensional structure, mapping of known single nucleotide polymorphisms (SNPs), identification of polymorphic microsatellite repeats within human genes, and comparative results with mouse full-length cDNAs. The H-InvDB analysis has shown that up to 4% of the human genome sequence (National Center for Biotechnology Information build 34 assembly) may contain misassembled or missing regions. We found that 6.5% of the human gene candidates(1,377 loci) did not have a good protein-coding open reading frame, of which 296 loci are strong candidates for nonprotein-coding RNA genes. In addition, among 72,027 uniquely mapped SNPs and insertions/deletions localized within human genes, 13,215 nonsynonymous SNPs, 315 nonsense SNPs, and 452 indels occurred in coding regions. Together with 25 polymorphic microsatellite repeats present in coding regions, they may alter protein structure, causingphenotypic effects or resulting in disease. The H-InvDB platform represents a substantial contribution to resources needed for the exploration of human biology and pathology.