著者

Seitaro OHTSU Masamitsu YAMAGUCHI Hisashi NISHIWAKI Eiichiro FUKUSAKI Shuichi SHIMMA

出版者

The Japan Society for Analytical Chemistry

雑誌

Analytical Sciences (ISSN:09106340)

巻号頁・発行日

vol.34, no.9, pp.991-996, 2018-09-10 (Released:2018-09-10)

参考文献数

19

被引用文献数

12

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Imidacloprid is widely used for exterminating harmful insects; however, information regarding its distribution in insects is limited. Herein, we developed a visualization method for imidacloprid in Drosophila melanogaster, by using matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS). IMS requires sample cryosections; however, certain challenges prevail in retaining fly morphology in sections owing to their small size and heterogeneous components. Therefore, the section preparation method was optimized first, followed by imidacloprid distribution visualized using MALDI-IMS. Using 10% gelatin as an embedding material and 70% ethanol for pretreatment, the gaps between embedding material and D. melanogaster body surface were reduced. The tight adhesion between embedding media and D. melanogaster retains fly morphology in sections. Furthermore, the imidacloprid standard was analyzed separately via MALDI and electrospray ionization (ESI), and imidacloprid was converted to guanidine-imidacloprid via laser irradiation. Consequently, the imidacloprid distribution in D. melanogaster was successfully visualized using guanidine-imidacloprid as the target peak.

著者

Seitaro OHTSU Masamitsu YAMAGUCHI Hisashi NISHIWAKI Eiichiro FUKUSAKI Shuichi SHIMMA

出版者

The Japan Society for Analytical Chemistry

雑誌

Analytical Sciences (ISSN:09106340)

巻号頁・発行日

pp.18SCP04, (Released:2018-06-29)

被引用文献数

12