著者

Hajime ASADA Masaya TSUBOI James K. CHAMBERS Kazuyuki UCHIDA Hirotaka TOMIYASU Yuko GOTO-KOSHINO Koichi OHNO Hajime TSUJIMOTO

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.17-0197, (Released:2017-09-01)

被引用文献数

12

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Canine histiocytic sarcoma (HS) is a malignancy originating from the histiocytic cell lineage and characterized by poor response to chemotherapy and short survival time. Mutation of the TP53 gene and its association with poor prognosis has been reported in several canine tumors. However, the mutation of this gene has not been investigated in canine HS. The aim of this study was to examine a TP53 gene mutation in dogs with HS. Aberrations of the TP53 gene were examined by polymerase chain reaction-single strand conformational polymorphism analysis and DNA sequence analysis, revealing mutations of the TP53 gene in 12 (46%) of 26 dogs affected by HS. The incidence of the TP53 gene mutation was relatively high in canine HS compared with other canine tumors. Among these mutations, 10 of 12 dogs (83%) with a TP53 gene mutation harbored the same mutation: a 2-base (AT) insertion in exon 5, resulting in the introduction of a stop codon (c.446_447insAT, p.Tyr150SerfsX8). Further studies are needed to examine the functional change due to the mutation and its association with the pathogenesis of canine HS.

著者

Daiki KATO Mariko OISHI Koichi OHNO Ko NAKASHIMA Atsuhito WADA Shin-ichiro FUKUMOTO Tatsushi MORITA Soichi IMAI Masaya TSUBOI James K. CHAMBERS Kazuyuki UCHIDA Hajime TSUJIMOTO

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.79, no.8, pp.e1, 2017 (Released:2017-08-04)

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NOTE Internal Medicine The first report of the ante-mortem diagnosis of Ollulanus tricuspis infection in two dogs Daiki KATO1), Mariko OISHI1), Koichi OHNO1)*, Ko NAKASHIMA1), Atsuhito WADA1), Tatsushi MORITA2), Soichi IMAI2), Masaya TSUBOI3), James K. CHAMBERS3), Kazuyuki UCHIDA3) and Hajime TSUJIMOTO1) 1)Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1–1–1 Yayoi, Bunkyo-ku, Tokyo 113–8657, Japan 2)Division of Veterinary Infectious Disease, Department of Veterinary Pathobiology, Nippon Veterinary and Life Science University, 1–7–1 Kyonanchyo Musashino, Tokyo 180–8602, Japan 3)Department of Veterinary Pathology, Graduate School of Agricultural Should have been NOTE Internal Medicine The first report of the ante-mortem diagnosis of Ollulanus tricuspis infection in two dogs Daiki KATO1), Mariko OISHI1), Koichi OHNO1)*, Ko NAKASHIMA1), Atsuhito WADA1), Shin-ichiro FUKUMOTO2), Tatsushi MORITA3), Soichi IMAI3), Masaya TSUBOI4), James K. CHAMBERS4), Kazuyuki UCHIDA4) and Hajime TSUJIMOTO1) 1),Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1–1–1 Yayoi, Bunkyo-ku, Tokyo 113–8657, Japan 2)Laboratory of Veterinary Parasitology, Department of Pathobiology, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, Japan 3)Division of Veterinary Infectious Disease, Department of Veterinary Pathobiology, Nippon Veterinary and Life Science University, 1–7–1 Kyonanchyo Musashino, Tokyo 180–8602, Japan 4)Department of Veterinary Pathology, Graduate School of Agricultural

著者

Mitsuteru OKAWAUCHI Masaya TSUBOI Kazumi NIBE Eiji NAGAMINE Hideaki IWANE Kazuyuki UCHIDA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.15-0721, (Released:2016-01-29)

被引用文献数

3

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An intraocular mass lesion was found in the left eyeball in a spayed female Pembroke Welsh Corgi dog. The surgically resected left eyeball was pathologically examined. Histologically, the mass lesion consisted of proliferation of the atypical cuboidal or columnar epithelial cells, arranging in papillary, tubular or solid form. In addition, some neoplastic cells showed oncocytic change characterized as large oval cells with numerous eosinophilic intracytoplasmic granules. Cytoplasm of the oncocytic cells showed dark blue granules by phosphotungstic acid-hematoxylin stain. Immunohistochemically, the oncocytic cells were intensely positive for cytochrome C. Based on these findings, the ocular mass was diagnosed as iridociliary adenocarcinoma with oncocytic change. The findings indicate that the oncocytic changes of the neoplastic epithelial cells might be caused by mitochondrial accumulation.

著者

Daiki KATO Mariko OISHI Koichi OHNO Ko NAKASHIMA Atsuhito WADA Tatsushi MORITA Soichi IMAI Masaya TSUBOI James Kenn CHAMBERS Kazuyuki UCHIDA Hajime TSUJIMOTO

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.15-0158, (Released:2015-06-05)

被引用文献数

1 6

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Ollulanus tricuspis is a small nematode parasite of the stomach, and its infection has been reported worldwide in cats but only one report in dogs as post-mortem diagnosis. Two dogs, kept in the Tokyo area, were presented for chronic vomiting. Chronic gastritis was diagnosed histologically, and many nematodes were detected in endoscopically-biopsied gastric samples and in the mucus of vomitus in both dogs. The parasites were small (<1 mm), and their morphological characteristics were consistent with those previously reported for O. tricuspis. The symptoms in one dog completely disappeared after anthelmintic therapy. To our knowledge, this is the first report describing ante-mortem diagnosis of spontaneous gastric O. tricuspis infection in dogs in which infectivity and pathogenicity of the nematode are suggested.