- 著者
- Kumakura Keiko Osanai Yuta Osa Yuichi Fujii Kei Asakawa Mitsuhiko Ushiyama Kii Onuma Manabu Igarashi Hirotaka Endoh Daiji
- 雑誌
- Japanese Journal of Veterinary Research (ISSN:00471917)
- 巻号頁・発行日
- vol.66, no.4, pp.261-272, 2018-11
There are a variety of purposes for identifying the different species of wild animals and birds. It needs a method that can save cost and time for the investigation of many birds. One of which is DNA barcoding that has been used for species identification from sampled muscle, feather or feces. In this study, we developed a novel nuclear exon-primed intron-crossing (EPIC) markers to distinct local birds in Hokkaido area. In birds, the size of introns is smaller than that of mammals. Many introns are within the size range that is suitable for EPIC. To design the EPIC primers, we collated the genome sequences of chicken, turkey, zebra finch and flycatcher on which genes and exons were annotated. We tested the primers for 16 introns which could amplify the introns in 12 different species birds. On the analysis of amplified introns, the size of polymorphisms has been shown in some species of birds. While an intron in TBC1D22B gene showed an interspecies difference in the sizes of amplified introns, some of the size difference was too small for reproducible observation. Since a species could not be identified with one gene, attempts were made to identify species by combining multiple genes. Resultantly, the amplified products of 3 introns have distinguished 10 species of birds. It has been noted that the EPIC method is capable of distinguishing 10 species of local birds in Hokkaido and is therefore suggested to be utilized as a convenient means for multiple species identification.
- 著者
- MAHMUT Halik GANZORIG Sumiya ONUMA Manabu MASUDA Ryuichi SUZUKI Masatsugu OHTAISHI Noriyuki
- 出版者
- The Graduate School of Veterinary Medicine
- 雑誌
- Japanese Journal of Veterinary Research (ISSN:00471917)
- 巻号頁・発行日
- vol.49, no.3, pp.231-237, 2001-11-30
To evaluate the genetic diversity of the Xinjiang Tarim red deer (Cervus elaphus yarkandensis) population, we analyzed the frequencies of microsatellite alleles. Samples were collected from 3 isolated populations in Xaya, Lopnur and Qarqan of Xinjiang. Although 10 microsatellite loci were examined, alleles of 133 to 190 base-pairs were detected for only 3 loci : BM5004, BM4208 and BM888. The average observed multilocus heterozygosity was 0.08±0.04 for the Xaya, 0 for the Lopnur, and 0.17±0.08 for the Qarqan population. The average heterozygosity of all populations was 0.08±0.02. The observed heterozygosities were significantly lower than the expected values. The present results suggest that the bottleneck effect has occurred in the populations of the Xinjiang Tarim red deer.