著者
Daisuke Nakajima Yusuke Kawashima Osamu Ohara
出版者
Japanese Proteomics Society
雑誌
Journal of Proteome Data and Methods (ISSN:24346454)
巻号頁・発行日
vol.5, pp.8, 2023 (Released:2023-04-29)
参考文献数
4

To analyse deep protein profiling of dried blood spot (DBS). We developed a simple method using sodium carbonate precipitation (SCP). SCP enriches hydrophobic proteins from DBS, allowing substantial removal of soluble proteins. In combination with SCP, we used quantitative LC-MS/MS proteome analysis in a data-independent acquisition mode (DIA) to enhance the sensitivity and quantification limits of proteome analysis.