This work demonstrates the fabrication of a nanoporous iron carbide-iron oxide/reduced graphene oxide (IC-IO/rGO) hybrid via a controlled one-step thermal treatment of Prussian blue (PB)/GO hybrid at 450 °C under N2 flow. The PB/GO hybrid is initially prepared through the in-situ deposition of PB nanoparticles on the GO sheets through electrostatic interactions. The morphological analysis of the hybrid reveals the uniform coverage of the rGO sheets by IC-IO nanoparticles and the even distribution of carbon (C), oxygen (O), and iron (Fe) on the rGO nanosheets. As a result of the hybrid composition and controlled morphology, the surface area of the obtained IC-IO/rGO hybrid (~40 m2/g) is significantly enhanced compared to those of the calcined GO sheets and PB nanoparticles (without GO).
A full-length cDNA clone encoding the flavoprotein (R)-(+)-mandelonitrile lyase was isolated from a black cherry (Prunus serotina) cDNA expression library and sequenced. A putative FAD-binding site was identified near the N-terminus of this enzyme by comparing its deduced amino acid sequence with those of other FAD- and NAD-binding proteins.