著者

Yoshiro Hadano Hajime Yoshii Michio Hayashi Hiroshi Oono Reiko Tanaka

出版者

The Japanese Society of Internal Medicine

雑誌

Internal Medicine (ISSN:09182918)

巻号頁・発行日

vol.54, no.9, pp.1141-1143, 2015 (Released:2015-05-01)

参考文献数

6

被引用文献数

1

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Cryptococcus arboriformis (C. arboriformis) is a novel Cryptococcus species belonging to the genus Trichosporonales. This novel species was identified definitively in 2007 using D1/D2 26S ribosomal DNA gene sequencing. In this article, we present a rare case of central line-associated bloodstream infection caused by C. arboriformis with successful treatment of this infection.

著者

田中 れい子 Reiko Tanaka 吉備国際大学社会福祉学部子ども福祉学科 Department of Child Welfare School of Social Welfare KIBI International University

出版者

高梁学園吉備国際大学

雑誌

吉備国際大学研究紀要 社会福祉学部 (ISSN:18836224)

巻号頁・発行日

no.20, pp.87-97, 2010

著者

Sayaka Nakamura Hiroaki Sato Reiko Tanaka Takashi Yaguchi

出版者

日本質量分析学会

雑誌

Mass Spectrometry (ISSN:2187137X)

巻号頁・発行日

vol.5, no.1, pp.A0049-A0049, 2016-11-10 (Released:2016-11-08)

参考文献数

28

被引用文献数

11

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We have previously proposed a rapid identification method for bacterial strains based on the profiles of their ribosomal subunit proteins (RSPs), observed using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). This method can perform phylogenetic characterization based on the mass of housekeeping RSP biomarkers, ideally calculated from amino acid sequence information registered in public protein databases. With the aim of extending its field of application to medical mycology, this study investigates the actual state of information of RSPs of eukaryotic fungi registered in public protein databases through the characterization of ribosomal protein fractions extracted from genome-sequenced Aspergillus fumigatus strains Af293 and A1163 as a model. In this process, we have found that the public protein databases harbor problems. The RSP names are in confusion, so we have provisionally unified them using the yeast naming system. The most serious problem is that many incorrect sequences are registered in the public protein databases. Surprisingly, more than half of the sequences are incorrect, due chiefly to mis-annotation of exon/intron structures. These errors could be corrected by a combination of in silico inspection by sequence homology analysis and MALDI-TOF MS measurements. We were also able to confirm conserved post-translational modifications in eleven RSPs. After these verifications, the masses of 31 expressed RSPs under 20,000 Da could be accurately confirmed. These RSPs have a potential to be useful biomarkers for identifying clinical isolates of A. fumigatus.