著者

HIROAKI SATO KYOHEI KAWAMURA KOJI HAYASHI HIROYUKI INAI TARO YAMAGUCHI

出版者

The Anthropological Society of Nippon

雑誌

Anthropological Science (ISSN:09187960)

巻号頁・発行日

pp.1201250125, (Released:2012-01-27)

被引用文献数

4 19

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We designed observational surveys of controlled foraging trips of Baka hunter-gatherers in Cameroon to verify the ‘wild yam question’—i.e. is it possible for human beings to live without agricultural products in a tropical rainforest?—and to examine their foraging lifestyle. We observed two 20-day trips during which no agricultural or commercial food except salt and pepper could be used. The first trip was conducted by six married couples in August, the short dry season, of the year 2003, and the second one by eight married couples in October, the rainy season, 2005. The Baka cooperators obtained 22 species and 43 vernacular names of food in all during both survey periods. No cooperators lost weight from any food shortage in both seasons. Energy intake per consumption-day was estimated at 2528–2865 kilocalories in the dry season, and at 2479–2777 kilocalories in the rainy season. Providing more than 60% of estimated energy intake in both seasons, wild yam tubers proved to be an essential food to enable a foraging life in tropical rainforests. From this survey we could find no evidence that it is impossible to live independently of agriculture in a tropical rainforest although it seemed that the cooperators paid a high energy cost to secure food, especially wild yam tubers. This study implies that a Paleolithic foraging lifestyle in the African tropical rainforest was very likely, although not easy, and that Paleolithic foragers may have been the ancestors of the present ‘pygmy’ hunter-gatherers.

著者

Hidekazu Yamazaki Masafumi Morimoto Chiyoe Hikita Mitsuhiro Iwasaki Masahiro Maeda Yasufumi Inaka Shinya Fukuta Hiroaki Sato

出版者

The Japanese Society for Neuroendovascular Therapy

雑誌

Journal of Neuroendovascular Therapy (ISSN:18824072)

巻号頁・発行日

pp.oa.2022-0035, (Released:2022-10-29)

参考文献数

14

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Objective: Mechanical thrombectomy (MT) for middle cerebral artery M2 occlusion (M2O) is challenging because the procedure is performed in a narrow and tortuous artery. In this study, we compared MT using an aspiration catheter (AC) versus a stent retriever (SR) used alone, and retrospectively evaluated the efficacy and safety of MT using an AC for M2O.Methods: Seventy-four consecutive patients who underwent MT for M2O at our institution between April 2016 and April 2020 were evaluated. The subjects were classified into those treated by AC (AC group) or SR alone (SR group). The AC group included patients treated by both contact aspiration and a combination technique of AC and SR. Background factors and outcomes, including modified treatment in cerebral infarction (mTICI) 2c-3 recanalization, were compared between the groups.Results: AC and SR groups consisted of 47 and 27 patients respectively. Among them, the rate of mTICI 2b-3 was 93.6% vs 92.6%, and that of mTICI 2c-3 was 72.3% vs 48.2% (P = 0.004). The perioperative symptomatic subarachnoid hemorrhage (SAH) rate was 0% vs 7.4%, and modified Rankin scale scores of 0–2 were 78.6% vs 50% (P = 0.03). In the AC group, the mTICI 2c-3 rate was higher in patients in whom the AC was adequately advanced to the thrombus compared to those with inadequate AC advancement (83.3% vs 36.3%, P = 0.002).Conclusion: The rate of mTICI 2c-3 was higher in the AC than SR group, with no cases of symptomatic SAH. MT using AC for M2O might achieve safe and effective thrombectomy.

著者

Tokuma Fukuoka Sayaka Nakamura Tomotake Morita Takayuki Ohmura Masahiro Kotani Yasuhide Naito Hiroaki Sato

出版者

Japan Oil Chemists' Society

雑誌

Journal of Oleo Science (ISSN:13458957)

巻号頁・発行日

pp.ess21084, (Released:2021-07-09)

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Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a promising tool for the screening of glycolipid-type biosurfactants (BSs) from a crude extract of microbial products. However, it is unsuitable for the detection of lower molecular weight products because the observed ions are overlapped with matrix-derived ions at lower mass range. In this study, we applied a “matrix-free” surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) analysis using a through-hole alumina membrane as an ionization-assisting substrate. Using this method, we could detect a variety of lower molecular weight products in an extract of a glycolipid BS producer with good sensitivity. In addition, the culture solution could be analyzed directly by this method.

著者

Sayaka Nakamura Hiroaki Sato Thierry N. J. Fouquet

出版者

The Mass Spectrometry Society of Japan

雑誌

Mass Spectrometry (ISSN:2187137X)

巻号頁・発行日

vol.9, no.1, pp.A0079, 2020-02-06 (Released:2020-02-06)

参考文献数

18

被引用文献数

2

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The Kendrick analysis is used for the processing and visualization of mass spectra obtained from polymers containing C, H, O and/or Si with simple isotopic patterns (monoisotope=lightest isotope=most intense isotope for short chains). In the case of heteroatoms with complex isotopic patterns, the impact of the chosen isotope on point alignments in Kendrick plots has not been examined extensively. Rich isotopic patterns also make the evaluation of the mass and nature of the repeating unit and end-groups more difficult from the mass spectrum in the case of unknown samples due to the number of peaks and the absence of a monoisotopic peak. Using a polybrominated polycarbonate as running example, we report that horizontal point alignments can be obtained in a Kendrick plot using the mass of the most abundant isotope instead of the monoisotopic mass as is usually done. Rotating the plot (“reverse Kendrick analysis”) helps to accurately evaluate the mass of the most abundant isotope of the repeating unit, as well as the nature of the brominated neutral expelled upon gentle heating (debromination or dehydrobromination). The whole procedure is then applied to the characterization of an unknown polybrominated flame retardant in an industrial formulation before and after heating.

著者

Sayaka Nakamura Hiroaki Sato Reiko Tanaka Takashi Yaguchi

出版者

日本質量分析学会

雑誌

Mass Spectrometry (ISSN:2187137X)

巻号頁・発行日

vol.5, no.1, pp.A0049-A0049, 2016-11-10 (Released:2016-11-08)

参考文献数

28

被引用文献数

11

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We have previously proposed a rapid identification method for bacterial strains based on the profiles of their ribosomal subunit proteins (RSPs), observed using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). This method can perform phylogenetic characterization based on the mass of housekeeping RSP biomarkers, ideally calculated from amino acid sequence information registered in public protein databases. With the aim of extending its field of application to medical mycology, this study investigates the actual state of information of RSPs of eukaryotic fungi registered in public protein databases through the characterization of ribosomal protein fractions extracted from genome-sequenced Aspergillus fumigatus strains Af293 and A1163 as a model. In this process, we have found that the public protein databases harbor problems. The RSP names are in confusion, so we have provisionally unified them using the yeast naming system. The most serious problem is that many incorrect sequences are registered in the public protein databases. Surprisingly, more than half of the sequences are incorrect, due chiefly to mis-annotation of exon/intron structures. These errors could be corrected by a combination of in silico inspection by sequence homology analysis and MALDI-TOF MS measurements. We were also able to confirm conserved post-translational modifications in eleven RSPs. After these verifications, the masses of 31 expressed RSPs under 20,000 Da could be accurately confirmed. These RSPs have a potential to be useful biomarkers for identifying clinical isolates of A. fumigatus.

著者

Go SEHATA Hiroaki SATO Toshihiro ITO Yoshitaka IMAIZUMI Taichi NORO Eiji OISHI

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.14-0066, (Released:2015-03-01)

被引用文献数

6

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We used real-time RT-PCR and virus titration to examine canine distemper virus (CDV) kinetics in peripheral blood and rectal and nasal secretions from 12 experimentally infected dogs. Real-time RT-PCR proved extremely sensitive, and the correlation between the two methods for rectal and nasal (r=0.78, 0.80) samples on the peak day of viral RNA was good. Although the dogs showed diverse symptoms, viral RNA kinetics were similar; the peak of viral RNA in the symptomatic dogs was consistent with the onset of symptoms. These results indicate that real-time RT-PCR is sufficiently sensitive to monitor CDV replication in experimentally infected dogs regardless of the degree of clinical manifestation and suggest that the peak of viral RNA reflects active CDV replication.

著者

Hiroaki Sato Yoko Ishii Hikaru Momose Takafumi Sato Kanae Teramoto

出版者

The Mass Spectrometry Society of Japan

雑誌

Mass Spectrometry (ISSN:2187137X)

巻号頁・発行日

vol.2, no.1, pp.A0014, 2013-02-01 (Released:2013-01-31)

参考文献数

22

被引用文献数

10 13

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The structural characterization of copolymers by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) remains a challenging task, since their random comonomer distribution creates very complicated mass spectra. In this study, a high-resolution TOF mass spectrometer with a spiral ion trajectory was applied to the structural and compositional characterization of free radical copolymerized poly(methyl methacrylate-co-tert-butyl methacrylate), poly(MMA-co-tBMA)s in ethyl lactate acting as a chain transfer agent. Virtually complete peak assignments of the isobaric components within the poly(MMA-co-tBMA)s served to identify the end-group combinations and copolymer compositions of individual copolymer components, allowing the distributions of comonomer compositions and six types of end-group combinations to be evaluated.