著者

OHMOMO Sadahiro AOSHIMA Ikuko TOZAWA Yukiko SAKURADA Noriko UEDA Kiyomoto

出版者

Japan Society for Bioscience, Biotechnology, and Agrochemistry

雑誌

Agricultural and Biological Chemistry (ISSN:00021369)

巻号頁・発行日

vol.49, no.7, pp.2047-2053, 1985

被引用文献数

11 19

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Melanoidin decolorizing enzymes (MDE) were extracted from mycelia of <i>Coriolus versicolor</i> Ps4a and purified by DEAE-Sephadex, DEAE-Sephacel and Sephadex G-200 column chromatographies. MDE of this strain consisted of a main fraction, P-fraction, and a minor fraction, E-fraction, and the P-fraction was composed of at least five enzymes. P-III and P-IV in the P-fraction were picked as typical enzymes of this strain, and their enzymatic properties were investigated. P-III had a molecular weight of 48, 400-50, 000, an optimum pH of 5.5 and an optimum temperature of 30-35°C. P-III required glucose and O₂ for the appearance of the activity, and was inhibited by <i>p</i>-CMB, <i>N</i>-BSI, Ag⁺ and <i>o</i>-phenanthroline.<br> On the other hand, P-IV had a molecular weight of 43, 800-45, 000, an optimum pH of 4.0-4.5 and an optimum temperature of 30-35°C. P-IV could decolorize melanoidin in the absence of glucose and O₂, and was inhibited weakly by Ag⁺, <i>p</i>-CME and <i>N</i>-BSI. P-IV is the enzyme that attacks the melanoidin directly in comparison with P-III which attacks melanoidin indirectly as in the sub-reaction of sugar oxidase.<br> Incidentally, a multiplicative effect between P-III and P-IV for decolorization was observed.